A5031438938- Asthma and respiratory diseases
- Blood Coagulation and Thrombosis Mechanisms
- Hearing, Cochlea, Tinnitus, Genetics
- Ear Surgery and Otitis Media
- Coagulation, Bradykinin, Polyphosphates, and Angioedema
- Ion Channels and Receptors
- Insect symbiosis and bacterial influences
- Invertebrate Immune Response Mechanisms
- Cancer Genomics and Diagnostics
- Allergic Rhinitis and Sensitization
- Bacteriophages and microbial interactions
- Protease and Inhibitor Mechanisms
- Platelet Disorders and Treatments
- Dermatology and Skin Diseases
- Hearing Loss and Rehabilitation
- Viral gastroenteritis research and epidemiology
- Plant Gene Expression Analysis
- Parvovirus B19 Infection Studies
- Salmonella and Campylobacter epidemiology
- Protein Kinase Regulation and GTPase Signaling
- Urticaria and Related Conditions
- Spatial Cognition and Navigation
- Augmented Reality Applications
- Animal Genetics and Reproduction
- Systemic Sclerosis and Related Diseases
Cornell University
2025
Weill Cornell Medicine
2025
Cincinnati Children's Hospital Medical Center
2023
Case Western Reserve University
2023
University of Alberta
1994-2017
Thermo Fisher Scientific (United States)
2015
Stanford University
2004-2012
Hammersmith Hospital
2003
Summary Background Proteinase‐activated receptor 2 ( PAR ) is a G protein‐coupled activated by trypsin‐like serine proteinases. activation has been associated with inflammation including allergic airway inflammation. We have also shown that in the airways leads to sensitization. The exact contribution of development eosinophilic and hyperresponsiveness AHR sensitized individuals not clear. Objective To investigate whether functional inhibition during allergen challenge mice would inhibit...
The goal of this study was to assess if mixed reality technology (MR) is a feasible training tool for educating new learners in endotracheal intubation. This feasibility trial establish the an MR airway education module compared traditional teaching. participants were twenty-one postgraduate year one (PGY1) physicians accepted emergency medicine residency program located large, urban setting. New York City, and has academic affiliations with two urban, departments (ED) that each treat over...
Summary In mammalian blood coagulation 5 proteases, factor VII (FVII), IX (FIX), X (FX), protein C (PC) and prothrombin act with two cofactors V VIII to control the generation of fibrin. Biochemical evidence molecular cloning data have previously indicated that involving tissue factor, fibrinogen is present in all vertebrates. Using degenerate RT-PCR we isolated characterized novel cDNAs sequence identity serine proteases from chicken puffer fish (Fugu rubripes). Sequence alignments,...
Fluorescent dye terminator Sanger sequencing (FTSS), with detection by automated capillary electrophoresis (CE), has long been regarded as the gold standard for variant detection. However, software analysis and base-calling algorithms used to detect mutations were largely optimized resequencing applications in which different alleles expected heterozygous mixtures of 50%. Increasingly, requirements are an analytic sensitivity minor <20%, particular, when assessing mutational status...
The duplication of genes and genomes is believed to be a major force in the evolution eukaryotic organisms. However, different models have been presented about how duplicated are preserved from elimination by purifying selection. Preservation one gene copies due rare mutational events that result new function (neofunctionalization) necessitates other copy retain its ancestral function. Alternatively, preservation both rapid divergence coding noncoding regions such neither retains complete...
Drosophila is a powerful model for molecular studies of hematopoiesis and innate immunity. However, its use functional cellular remains hampered by the lack single-cell assays hemocytes (blood cells). Here we introduce generic method combining fluorescence-activated cell sorting nonantibody probes that enables selective gating live from lymph glands (larval hematopoietic organ) or hemolymph equivalent). Gated are analyzed sorted at will based on precise quantitation fluorescence levels...
Pendred syndrome (PDS) and DFNB4 comprise a phenotypic spectrum of sensorineural hearing loss disorders that typically result from biallelic mutations the SLC26A4 gene. Although PDS are recessively inherited, sequencing coding regions splice sites in individuals suspected to be affected with these conditions often fails identify two mutations. We investigated potential contribution large deletions duplications (SNHL) by screening 107 probands one known mutation Multiplex Ligation-dependent...
Following a case of Campylobacter fetus sepsis and meningitis in 4-month-old female member Hutterite colony, an epidemiological investigation revealed at least 18 cases diarrhea other members the colony. C. was isolated from 7 15 fecal samples submitted affected persons. A control study suggested that persons who worked abattoir were 2.03 times more likely to have had diarrhea, but none risk factors studied significant. The epicurve outbreak inconclusive as mode spread fetus. All strains...
Summary Gene duplication is an important means of generating new genes. The major mechanisms by which duplicated genes are preserved in the face purifying selection thought to be neofunctionalization, subfunctionalization, and increased gene dosage. However, very few families vertebrate species have been analyzed functional tests vivo. We therefore examined three Myb (c-Myb, A-Myb, B-Myb) cytogenetic map analysis, sequence ectopic expression Drosophila. provide evidence that arose two rounds...
Abstract Background CRT h2 (chemoattractant‐receptor homologous molecule expressed on T cells) is by cells and other involved in allergic inflammation. Single nucleotide polymorphisms ( SNP s) CRTh2 (rs11571288, rs545659, rs634681) have been associated with various phenotypes of allergy ethnically distinct populations. Here, we assessed the association between rs533116 asthma, expression cytokine production. Methods was genotyped an diverse population n = 1282). The proportion expressing...
Abstract Background Proteinase‐Activated Receptor‐2 ( PAR 2 ) is a G protein‐coupled receptor activated by serine proteinases. We have shown that activation in the airways involved development of allergic inflammation and airway hyperresponsiveness AHR acute murine models. hypothesized functional inhibition prevents inflammation, remodeling chronic Material Methods developed used 12 week model cockroach extract CE )‐mediated , BALB /c mice. Results Mice sensitized challenged with for weeks...
Pendred syndrome (PDS) and DFNB4 comprise a phenotypic spectrum of sensorineural hearing loss disorders that typically result from biallelic mutations the SLC26A4 gene. Although PDS are recessively inherited, sequencing coding regions splice sites in individuals suspected to be affected with these conditions often fails identify two mutations. We investigated potential contribution large deletions duplications (SNHL) by screening 107 probands one known mutation Multiplex Ligation-dependent...
Abstract By increasing quality-associated parameters and lowering sensitivity thresholds, a significant improvement in low frequency allele detection was achieved. The approach described optimized using dilution series of synthetic spike-ins (the AcroMetrix® Oncology Hotspot Control), titrated range tumor cell lines normal background. variant calling then applied to the analysis somatic variants circulating (CTC) samples associated cell-free DNA (cfDNA) next-generation sequencing (NGS)....
Pendred syndrome (PDS) and DFNB4 comprise a phenotypic spectrum of sensorineural hearing loss disorders that typically result from biallelic mutations the SLC26A4 gene. Although PDS are recessively inherited, sequencing coding regions splice sites in individuals suspected to be affected with these conditions often fails identify two mutations. We investigated potential contribution large deletions duplications (SNHL) by screening 107 probands one known mutation Multiplex Ligation-dependent...