Chemoattractant receptor-homologous molecule expressed on Th2 cells (CRTh2) is a receptor for PGD2 and by T cells, eosinophils, basophils, ILC2 cells. CRTh2 expression CD4(+) identifies the subset, these have been characterized as allergen-specific central memory Recently, activation of -CRTh2 pathway in lungs was associated with severe asthma.To assess circulating levels related mediators asthma those who experience exacerbations.Peripheral blood expressing were flow cytometry qRT-PCR....

Introduction Antibiotics are among the most commonly prescribed drugs in pediatrics. Because of an overall rise health care costs, lack uniformity drug prescribing and emergence antibiotic resistance, monitoring control use growing concern strict policies should be warranted. Before such can implemented, detailed knowledge practice is important. Objective The main objective to study hospital in-patient. Methods A retrospective one half month’s duration was undertaken during NovemberDecember...

Purpose: Aspirin-intolerant asthma (AIA) is characterized by moderate to severe that aggravated aspirin or other non-steroidal antiinflammatory drugs.Affected patients frequently have chronic rhinosinusitis and nasal polyposis due persistent upper lower airway inflammation with marked eosinophilia.IL-13 plays a crucial role in the development of allergic inducing eosinophilia hyper-reactivity it has been correlated an increased eosinophil count.Methods: Two promoter polymorphisms IL-13 gene...

THREE DIISOCYANATES CAN CAUSE OCCUPATIONAL ASTHMA (OA): toluene diisocyanate (TDI), 4,4 diphenylmethane (MDI), and 1,6-hexamethylene (HDI). We analyzed potential biomarkers of isocyanate-induced OA, based on investigated immunologic, genetic, neurogenic, protein markers, because there is no serological testing method. The prevalence serum IgG to cytokeratin (CK)18 CK19 in TDI-OA was significantly higher than controls, although the these antibodies too low for them be used as biomarkers....

To cite this article: Palikhe NS, Kim S‐H, Cho B‐Y, Ye Y‐M, Choi G‐S, Park H‐S. Genetic variability in CRTH2 polymorphism increases eotaxin‐2 levels patients with aspirin exacerbated respiratory disease. Allergy 2010; 65 : 338–346. Abstract Introduction: is expressed on the surface of eosinophils and has been shown to mediate PGD2‐induced eosinophil migration vitro . Eosinophilic infiltration upper lower airways key feature asthma. Considering fact that prominent disease (AERD) compared...

Cite this as: N. S. Palikhe, S.‐H. Kim,H.‐Y. Lee, J.‐H. Kim, Y.‐M. Ye and H.‐S. Park, Clinical & Experimental Allergy, 2011 (41) 179–185. Summary Background The thromboxane A2 receptor ( TBXA2R ) is a potent broncho‐ vaso‐constrictor associated with leukotriene synthesis. Polymorphisms in the gene have been linked to atopy, asthma, atopic dermatitis. This study evaluated association between genetic variants development of acetyl salicylic acid (ASA)‐intolerant acute urticaria (AIAU)....

CRTh2 (encoded by PTGDR2) is a G-protein coupled receptor expressed Th2 cells as well eosinophils, basophils and innate lymphoid (ILC)2s. Activation of CRTh2, its ligand prostaglandin (PG)D2, mediates production type 2 cytokines (IL-4, IL-5 IL-13), chemotaxis inhibition apoptosis. As such, the PGD2-CRTh2 pathway considered important to development maintenance allergic inflammation. Expression mediated transcription factor GATA3 during cell differentiation within ILC2s. Other than this,...

Chronic obstructive pulmonary disease (COPD) affects up to 10% of Canadians. Patients with COPD may present secondary humoral immunodeficiency as a result chronic disease, poor nutrition or frequent courses oral corticosteroids; decreased immunity predispose these patients mucosal infections. We hypothesized that serum immunoglobulin (Ig) levels was associated the severity an acute exacerbations (AECOPD).A prospective study examine cardiovascular risks in hospitalized for AECOPD, recruited...

Introduction: Chronic urticaria (CU), defined as the recurring incidence of wheals with or without angioedema for more than 6 weeks, is a common disorder associated mast cell activation, degranulation, and histamine release. Considering association between CRTH2 gene cells, we investigated this polymorphism CU phenotype antihistamine drug requirement in patients CU. Materials & methods: Two groups consisting 384 231 normal controls (NCs) were enrolled from Department Allergy Rheumatology,...

origin, have been noted in AERD patients. 5In another study, exacerbation of with airway infection respiratory syncytial virus was reported. 6lthough there are a few published studies on the association between viral and AERD, no clear mechanism(s) behind virus-induced has described.One possible explanation is direct activation by components through TLRs.The TLR family comprises TLR1-TLR10, each which recognizes conserved microbial structure then initiates host defense response. 7,8The...

Background Protease-Activated Receptor-2 (PAR-2), a G protein coupled receptor activated by serine proteases, is widely expressed in humans and involved inflammation. PAR-2 activation the airways plays an important role development of allergic airway expression known to be upregulated epithelium asthmatic subjects, but its on immune inflammatory cells patients with asthma has not been studied. Methods We recruited 12 severe 24 mild/moderate asthmatics from University Alberta Hospital Asthma...

Abstract Background Allergic asthma is characterized by type 2 inflammation. We have shown the presence of increased inflammation in patients with severe and those frequent exacerbations. However, it not known whether drives This study aims to determine Th2 immune parameters presenting emergency department (ED) an acute exacerbation correlate these clinical physiological measures asthma. Methods Sixteen adults ED exacerbations were recruited after giving informed consent. Ten returned weeks...

Aspirin ingestion can induce a wide range of clinically recognized allergic reactions, including acetyl salicylic acid (ASA)exacerbated respiratory disease (AERD), ASA-intolerant urticaria (AIU), chronic rhinitis, and anaphylaxis.Patients with AIU have high rates atopy increased total IgE levels, 1 but the pathogenic mechanism remains unclear.ASA is metabolized by UDP-glucuronosyltransferase 1A6 (UGT1A6), cytochrome P4502C9 (CYP2C9), N-acetyl transferase 2 (NAT2).Inter-individual differences...

Abstract Background CRT h2 (chemoattractant‐receptor homologous molecule expressed on T cells) is by cells and other involved in allergic inflammation. Single nucleotide polymorphisms ( SNP s) CRTh2 (rs11571288, rs545659, rs634681) have been associated with various phenotypes of allergy ethnically distinct populations. Here, we assessed the association between rs533116 asthma, expression cytokine production. Methods was genotyped an diverse population n = 1282). The proportion expressing...

Abstract Human CRTh2 + Th2 cells express IL‐25 receptor (IL‐25R) and has been shown to potentiate production of cytokines. However, regulation IL‐25R whether it participates in differentiation human have not examined. We sought characterize expression on CD4 T determine plays a role differentiation. Naïve were activated the presence IL‐25, IL‐4 (Th2 conditions) or both cytokines assess their relative influence For experiments with differentiated cells, CRTh2‐expressing isolated from...

Abstract Background Glucocorticosteroids (GCs) are the main treatment for asthma as they reduce type 2 cytokine expression and induce apoptosis. Asthma severity is associated with inflammation, circulating Th2 cells higher GC requirements. Objective The aim of this study was to assess whether ex vivo production interleukin (IL‐2), a T‐cell survival factor, clinical features severity, proportion blood cell responses GC. Methods Peripheral from patients (n = 18) obtained determined by flow...

Abstract Background Type 2‐high asthma is characterized by elevated levels of circulating Th2 cells and eosinophils, that express chemoattractant‐homologous receptor expressed on (CRTh2). Severe more common in women than men; however, the underlying mechanism(s) remain elusive. Here we examined whether relationship between severe type 2 inflammation differs sex if estrogen influences cell response to glucocorticoid (GC). Methods proportion blood (CD4 + CRTh2 ) were assessed whole from...

Summary Background Allergic rhinitis (AR) is a very common disease and risk factor for allergic asthma. The discovery of new biomarkers the early detection AR would improve clinical outcomes reduce socio‐economic burden. We sought to identify novel serologic marker using proteomic approach. Methods To proteins involved in AR, comparative proteomics was applied nasal lavage fluids (NLFs) taken before after provocation test (NPT) with Dermatophagoides pteronyssinus ( Dpt ) subject sensitized ....