A5017029498- Mast cells and histamine
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Immune cells in cancer
- Asthma and respiratory diseases
- Cytokine Signaling Pathways and Interactions
- Immune Response and Inflammation
- Reproductive System and Pregnancy
- Click Chemistry and Applications
- Cancer Research and Treatments
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer Immunotherapy and Biomarkers
- Inflammatory Biomarkers in Disease Prognosis
- Chemokine receptors and signaling
- Prostate Cancer Treatment and Research
- Immunotherapy and Immune Responses
- IL-33, ST2, and ILC Pathways
- Urticaria and Related Conditions
- Cytomegalovirus and herpesvirus research
- Antimicrobial Peptides and Activities
- Pregnancy and Medication Impact
- interferon and immune responses
- Food Allergy and Anaphylaxis Research
- Viral-associated cancers and disorders
- Glioma Diagnosis and Treatment
University of Bologna
2023-2024
University of Udine
2009-2018
Veneto Institute of Molecular Medicine
2003-2005
University of Padua
2004-2005
Nephrologisches Zentrum Goettingen
2004
Fondazione IRCCS Istituto Nazionale dei Tumori
2000-2003
The Wistar Institute
1996-2000
Children's Hospital of Philadelphia
1999
Philadelphia Fight
1999
University of Modena and Reggio Emilia
1996
Interleukin (IL) 12 is a proinflammatory cytokine produced by phagocytic cells, B and other antigen-presenting cells that modulates adaptive immune responses favoring the generation of T helper type 1 cells. IL-12 mediates some its physiological activities acting as potent inducer interferon (IFN) gamma production natural killer IFN-gamma enhances ability to produce cytokines. Thus, IL-12-induced acts in positive feedback loop represents an important amplifying mechanism inflammatory...
Immunotherapy may provide valid alternative therapy for patients with hormone-refractory metastatic prostate cancer. However, if the tumor environment exerts a suppressive action on antigen-specific tumor-infiltrating lymphocytes (TIL), immunotherapy will achieve little, any, success. In this study, we analyzed modulation of TIL responses by using collagen gel matrix–supported organ cultures human carcinomas. Our results indicate that prostatic adenocarcinomas are infiltrated terminally...
Expression of a dominant negative mutant IFNgammaR1 in murine SCK and K1735 tumor cells rendered them relatively unresponsive to IFNgamma vitro more tumorigenic less responsive IL-12 therapy vivo. induced histologic evidence ischemic damage only IFNgamma-responsive tumors, vivo Matrigel vascularization assays revealed that while -unresponsive angiogenesis equally well, its downstream mediator inhibited by the cells. inhibitory activity cells, which may be attributable production chemokine...
Abstract Human NK cells cultured in the presence of IL-12 or IL-4 differentiate into cell populations with distinct patterns cytokine secretion similar to Th1 and Th2 cells. grown (NK1) produce IL-10 IFN-γ, whereas (NK2) IL-5 IL-13. Although these subsets do not differ cytotoxic activity, NK1 express higher levels surface CD95 (Fas) Ag than NK2 are more sensitive Ab chemically induced apoptosis. Like cells, accumulate much IL-12Rβ2-chain mRNA significantly responsive at level activation...
A major contributing factor to glioma development and progression is its ability evade the immune system. Nano-meter sized vesicles, exosomes, secreted by glioma-stem cells (GSC) can act as mediators of intercellular communication promote tumor escape. Here, we investigated immunomodulatory properties GCS-derived exosomes on different peripheral cell populations. Healthy donor blood mononuclear (PBMCs) stimulated with anti-CD3, anti-CD28 IL-2, were treated GSC-derived exosomes. Phenotypic...
Mast cells (MCs) are currently recognized as effector in many settings of the immune response, including host defense, regulation, allergy, chronic inflammation, and autoimmune diseases. MC pleiotropic functions reflect their ability to secrete a wide spectrum preformed or newly synthesized biologically active products with pro-inflammatory, anti-inflammatory and/or immunosuppressive properties, response multiple signals. Moreover, modulation phenotypes relies on interaction variety membrane...
Interleukin-12 (IL-12) is a proinflammatory cytokine produced by antigen-presenting cells in response to many microbial infections. IL-12 plays an important role the generation of T helper type-1 cells, which favor cell-mediated immune response. composed two different subunits, p40 and p35, whose expression can be regulated concomitantly or differentially. Monocytic major producers IL-12, primed interferon-γ (IFN-γ) produce optimal amounts LPS stimulation as consequence bacterial infection....
Translational medicine aims at transferring advances in basic science research into new approaches for diagnosis and treatment of diseases. Low-grade gliomas (LGG) have a heterogeneous clinical behavior that can be only partially predicted employing current state-of-the-art markers, hindering the decision-making process. To deepen our comprehension on tumor heterogeneity, we dissected mechanism interaction between cells relevant components neoplastic environment, isolating, from LGG...
Abstract Inflammation plays crucial roles at different stages of tumor development and may lead to the failure immune surveillance immunotherapy. Myeloid-derived suppressor cells (MDSC) are one major components immune-suppressive network that favors growth, their interaction with mast is emerging as critical for outcome tumor-associated response. Herein, we showed occurrence cell-to-cell interactions between MDSCs in mucosa patients colon carcinoma spleen tumor-bearing mice. Furthermore,...
Abstract IL-12 activates murine and human B cells, but little information is available as to the expression function of IL-12R on lymphocytes. Here we show that latter freshly isolated from tonsils, expressed transcripts both β1 β2 chains chain mRNA was selectively increased (4- 5-fold) by incubation with Staphylococcus aureus Cowan I bacteria or IL-12. cell stimulation induced de novo two IL-18R, i.e., IL-1 receptor-related protein accessory protein-like. Functional studies showed IL-18...
Abstract In T lymphocytes, the Src family kinase Lck associates lipid rafts and accumulates at immunological synapse (IS) during cell stimulation by APCs. Using CD4- or CD28-deficient murine cells, it was suggested that recruitment of to IS depends on CD4, whereas CD28 sustains activation. However, in human resting is responsible for promoting an unknown mechanism. Thus, we performed a series experiments determine 1) whether recruited through rafts; 2) cells CD4 engagement. We found CD28,...
Abstract The aryl hydrocarbon receptor (AhR) is a ligand-dependent transcription factor whose activity modulated by xenobiotics as well physiological ligands. These compounds may modulate inflammatory responses and contribute to the rising prevalence of allergic diseases observed in industrialized countries. Mast cells (MCs), located within tissues at boundary external environment, represent potential target AhR In this study, we report that murine human MCs constitutively express AhR, its...
Abstract Efficient T cell priming by GM-CSF and CD40 ligand double-transduced C26 murine colon carcinoma is not sufficient to cure metastases in a therapeutic setting. To determine whether cellular vaccine that interacts directly with both APC cells vivo might be superior, we generated transduced the costimulatory molecule OX40 (OX40L) either alone (C26/OX40L) or together (C26/GM/OX40L), which known activate APC. Mice injected C26/OX40L displayed only delay tumor growth, while C26/GM/OX40L...