A5101547371- Antibiotic Resistance in Bacteria
- Mycobacterium research and diagnosis
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Streptococcal Infections and Treatments
- Infectious Diseases and Tuberculosis
- Tuberculosis Research and Epidemiology
- Plant Pathogenic Bacteria Studies
- Clostridium difficile and Clostridium perfringens research
- Bacteriophages and microbial interactions
- Bacillus and Francisella bacterial research
- Diphtheria, Corynebacterium, and Tetanus
- Otolaryngology and Infectious Diseases
- Infections and bacterial resistance
- Vibrio bacteria research studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Microscopic Colitis
- Bacterial biofilms and quorum sensing
- Enterobacteriaceae and Cronobacter Research
- Antibiotics Pharmacokinetics and Efficacy
- Sinusitis and nasal conditions
- Mycorrhizal Fungi and Plant Interactions
- Plant-Microbe Interactions and Immunity
- Gut microbiota and health
- Neonatal and Maternal Infections
The Verigene Gram-positive blood culture test (BC-GP) and the Gram-negative (BC-GN) identify representative bacteria, bacteria their antimicrobial resistance by detecting genes within 3 h. Significant benefits are anticipated due to rapidity accuracy, however, clinical utility is unproven in studies. We performed a trial between July 2014 December for hospitalized bacteremia patients. During intervention period (N = 88), BC-GP BC-GN was used along with conventional microbiological diagnostic...
This study was performed to investigate the carriage rates of CTX-M-type extended-spectrum β-lactamase (ESBL)-producing Escherichia coli among ill companion animals in Japan. Among 178 nonrepetitive E. isolates, including 131 from dogs and 47 cats, collected between September November 2015, 42 (23.6%) isolates 29 13 cats were identified as ESBL producers. The antimicrobial susceptibility, O serotype, phylogenetic group, genotype, plasmid replicon type, sequence type (ST) each isolate...
ABSTRACT Metallo-β-lactamases (MBLs) are transmissible carbapenemases of increasing prevalence in Gram-negative bacteria among health care facilities worldwide. Control the further spread these carbapenem-resistant relies on clinical microbiological laboratories correctly identifying and classifying MBLs. In this study, we evaluated a simple rapid method for detecting IMP, most prevalent MBL Japan. We used an immunochromatography (IC) assay 181 carbapenem-nonsusceptible (CNS) (nonsusceptible...
The rapid identification of acid-fast bacilli recovered from patient specimens as Mycobacterium tuberculosis complex (MTC) is critically important for accurate diagnosis and treatment. A thin-layer immunochromatographic (TLC) assay using anti-MPB64 or anti-MPT64 monoclonal antibodies was developed to discriminate between MTC non-tuberculosis mycobacteria (NTM). Capilia TB-Neo, which the improved version TB, recently needs be evaluated. TB-Neo evaluated by reference strains including 96...
We characterized the profiles of virulence genes and antimicrobial susceptibility Bacillus cereus isolates from blood cultures as well risk factors for stream infections (BSIs). The diversity gene patterns was found to be wide among 15 B. BSIs also 11 contaminated cultures. MicroScan broth microdilution method yielded results corresponding with those agar dilution (reference) levofloxacin, linezolid, vancomycin, while Etest were consistent reference clindamycin, gentamicin, imipenem,...
A 62-year-old Japanese woman was hospitalized at the Department of Senology for positive signals on two sets blood cultures obtained in Emergency Department. The initial physical examination with enhanced computed tomography chest and abdomen did not identify infectious source. Dialister pneumosintes identified 16S rRNA sequencing, dental caries sinusitis were subsequently diagnosed based a magnetic resonance imaging. History taking respect to hygiene oral examinations should be performed...
To characterise the genotypic profiles of methicillin-resistant Staphylococcus aureus (MRSA) clinical isolates from companion animals and to investigate their association with those humans in Japan. Non-duplicated MRSA recovered between July 2016 January 2018 were analysed. The typed by polymerase chain reaction (PCR)-based open reading frame (ORF) typing (POT) scores, SCCmec types, multilocus sequence typing, virulence gene profiles. Phylogenetic comparison previously described human was...
Abstract We investigated the efficiency of Verigene Enteric Pathogens Nucleic Acid Test (Verigene EP test), which is an automated microarray-based assay system that enables rapid and simultaneous genetic detection gastrointestinal pathogens toxins, including those in Campylobacter Group, Salmonella species, Shigella Vibrio Yersinia enterocolitica , Shiga toxin 1 2, norovirus GI/GII, rotavirus A. Three clinical laboratories evaluated test, using 268 stool samples for bacterial genes 167 viral...
Central nervous system infections caused by Bacillus cereus have rarely been reported in infants. In this paper, the case of a 2-month-old low-birth-weight female who developed meningitis 45 days after resolution bloodstream infection (BSI) is described. The pulsed-field gel electrophoresis results revealed that patterns both B. isolates responsible for acute and prior bacteraemic episode were closely related. Although source from within patient was not clear, it suggested BSI neonate...
A 56-year-old previously healthy man was hospitalized due to a 10-day history of neck pain and an elevated C-reactive protein level. Gram-negative spiral bacilli were isolated from his blood, Helicobacter cinaedi confirmed using 16S rRNA sequencing. The infectious focus not identified by initial cervical magnetic resonance imaging (MRI); however, repeated MRI demonstrated prominent high signal intensity in the entire region C6-C7 vertebrae C6/C7 disc space. Furthermore,...
Recently, a new rapid assay for the detection of tcdB gene Clostridioides difficile was developed using GENECUBE. The can directly detect from stool samples without purification in approximately 35 minutes with few preparation process. We performed prospective comparative study performance at eight institutions Japan. Fresh residual (Bristol scale ≥5) were used and comparisons BD MAX Cdiff toxigenic cultures. For evaluation 383 compared assay, sensitivity, specificity two assays 99.0%...
The Enterobacter cloacae complex (ECC) is one of the most common causes bacteremia and leads to poor clinical outcomes. aim this study was clarify antimicrobial susceptibility profiles genetic backgrounds non-carbapenemase-producing reduced-carbapenem-susceptible (RCS) ECC blood isolates in Japan using agar dilution testing, whole-genome sequencing, quantitative polymerase chain reaction for ampC, ompC, ompF transcripts. Forty-two were categorized into RCS carbapenem-susceptible groups based...
ObjectivesA standard treatment regimen against Mycobacteroides abscessus complex (MABC) infections has not yet been established, making MABC difficult to treat successfully. In this study, we sought develop an active ingredient for the clinical of infections.MethodsWe screened 102 strains isolated from specimens using DNA sequence analysis with housekeeping genes hsp65 and rpoB. Drug susceptibility testing was performed two subspecies—Mycobacteroides subsp. (M. abscessus) massiliense...
A 43-year-old man was referred to our hospital for an acute-onset fever and left flank pain. He had been previously diagnosed with lymphangioma, abdominal computed tomography showed pararenal cysts fat stranding around the kidney, of which infection subsequently confirmed on magnetic resonance imaging. Gram-negative spiral bacilli were isolated from two sets blood cultures, Helicobacter cinaedi identified using 16S rRNA sequencing. The patient successfully treated ceftriaxone therapy without...
Introduction: Cavernous sinus thrombosis (CST) is a rare manifestation of cerebral venous thrombosis. We herein describe the first case CST associated with oral microbes (Dialister pneumosintes, Slackia exigua and Prevotella baroniae). Case presentation: An 82‐year‐old previously self‐supported Japanese woman presented acute onset impaired consciousness. Computed tomography revealed intracranial haemorrhage complicated CST. D. P. baroniae were cultivated from two sets blood cultures...