A5072217209- Mycobacterium research and diagnosis
- Tuberculosis Research and Epidemiology
- Infectious Diseases and Mycology
- Infectious Diseases and Tuberculosis
- Bacteriophages and microbial interactions
- Pneumocystis jirovecii pneumonia detection and treatment
- Diagnosis and treatment of tuberculosis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Genomics and Phylogenetic Studies
- Antibiotic Resistance in Bacteria
- Bacterial Identification and Susceptibility Testing
- Actinomycetales infections and treatment
- Pneumonia and Respiratory Infections
- Cholangiocarcinoma and Gallbladder Cancer Studies
- Primate Behavior and Ecology
- Medical Research and Treatments
- Cell Image Analysis Techniques
- Enterobacteriaceae and Cronobacter Research
- Quinazolinone synthesis and applications
- Escherichia coli research studies
- Phenothiazines and Benzothiazines Synthesis and Activities
- Plant Pathogenic Bacteria Studies
- Bat Biology and Ecology Studies
- Microfluidic and Capillary Electrophoresis Applications
- Chronic Lymphocytic Leukemia Research
Japan Anti Tuberculosis Association
2015-2024
BACKGROUND. Inadequate tuberculosis (TB) diagnostics are a major hurdle in the reduction of disease burden, and accurate point-of-care tests (POCTs) urgently needed. We assessed diagnostic accuracy Fujifilm SILVAMP TB lipoarabinomannan (FujiLAM) POCT for diagnosis HIV-negative outpatients compared it with Alere Determine LAM Ag (AlereLAM) laboratory-based ultrasensitive electrochemiluminescence research assay (EclLAM).
There is an urgent need for rapid, non-sputum point-of-care diagnostics to detect tuberculosis. This prospective trial in seven high tuberculosis burden countries evaluated the diagnostic accuracy of urine-based lipoarabinomannan assay FUJIFILM SILVAMP TB LAM (FujiLAM) among inpatients and outpatients living with HIV. Diagnostic performance FujiLAM was assessed against a mycobacterial reference standard (sputum culture, blood Xpert Ultra from urine sputum at enrollment, additional culture ≤7...
Abstract Performance of interferon-γ (IFN-γ) release assays still needs to be improved. The data on the performance QuantiFERON-TB Gold Plus (QFT-Plus), a new-generation QFT assay are limited. This study evaluated diagnostic QFT-Plus, and compared that In-Tube (QFT-GIT). Blood samples were collected from 162 bacteriologically confirmed tuberculosis (TB) patients 212 Mycobacterium -uninfected volunteers; these then tested with QFT-GIT QFT-Plus. IFN-γ concentration QFT-Plus was lower than in...
Abstract Background The novel Fujifilm SILVAMP TB-LAM (FujiLAM) assay detects mycobacterial lipoarabinomannan in urine and has demonstrated superior sensitivity to the Alere Determine Ag (AlereLAM) for detection of tuberculosis among hospitalized people with human immunodeficiency virus (PWH). This is first study evaluate a broad population referred antiretroviral therapy including both outpatients (mainly) inpatients. Methods We assessed diagnostic accuracy FujiLAM AlereLAM assays biobanked...
We evaluated a new line probe assay (LiPA) kit to identify Mycobacterium species and detect mutations related drug resistance in tuberculosis. A total of 554 clinical isolates tuberculosis (n = 316), avium 71), intracellulare 51), kansasii 54), other 62) were tested with the LiPA six hospitals. The was also used directly test 163 sputum specimens. results identification almost identical those conventional methods. Compared standard susceptibility testing for isolates, showed sensitivity...
We previously reported the exquisite preservation of ultrastructures virulent Mycobacterium tuberculosis cells processed through cryofixation and rapid freeze substitution. Here, we report "structome" analysis (i.e., quantitative three-dimensional structural a whole cell at electron microscopic level) M. using serial ultrathin sections prepared after substitution analyzed by transmission microscopy. Five cells, which were contained in cross encompassing from one end to other, cut into 24,...
The clinical importance of Mycobacterium abscessus species (MABS) infections has been increasing. However, the standard treatment regimens recommended in current guidelines often result unfavorable outcomes. Therefore, we investigated vitro activity omadacycline (OMC), a novel tetracycline, against MABS to explore its potential as therapeutic option. drug susceptibilities 40 subsp. (Mab) strains obtained from sputum patients January 2005 May 2014 were investigated. MIC results for OMC,...
Abstract The emergence and spread of multidrug-resistant (MDR) extensively drug-resistant (XDR) tuberculosis (TB) has raised public health concern about global control TB. To estimate the transmission dynamics MDR XDR TB, we conducted a DNA fingerprinting analysis 55 MDR/XDR Mycobacterium strains isolated from TB patients throughout Japan in 2002. Twenty-one (38%) were classified into 9 clusters with geographic links, which suggests that community is ongoing. Furthermore, M. more likely than...
The rapid identification of acid-fast bacilli recovered from patient specimens as Mycobacterium tuberculosis complex (MTC) is critically important for accurate diagnosis and treatment. A thin-layer immunochromatographic (TLC) assay using anti-MPB64 or anti-MPT64 monoclonal antibodies was developed to discriminate between MTC non-tuberculosis mycobacteria (NTM). Capilia TB-Neo, which the improved version TB, recently needs be evaluated. TB-Neo evaluated by reference strains including 96...
ObjectivesThis study examined Mycobacterium tuberculosis (MTB)-secreted MPT64 as a surrogate of bacterial viability for the diagnosis active pulmonary TB (PTB) and follow-up treatment.MethodsIn this proof-of-concept prospective study, 50 PTB patients in Tokyo metropolitan region, between 2017 2018, were consecutively included 30 healthy individuals also included. Each patient submitted sputum on days 0, 14 28 follow-up, each individual one sample. The following performed: smear microscopy,...
Series of structome analyses, that is, quantitative and three-dimensional structural analysis a whole cell at the electron microscopic level, have been already achieved individually in Exophiala dermatitidis, Saccharomyces cerevisiae, Mycobacterium tuberculosis, Myojin spiral bacteria Escherichia coli. In these analysis, sample cells were processed through cryo-fixation rapid freeze-substitution, resulting exquisite preservation ultrastructures on serial ultra-thin sections examined by...
Abstract Rationale There is an urgent need for rapid, non-sputum point-of-care diagnostics to detect tuberculosis. Objectives This prospective trial in seven high tuberculosis burden countries set out evaluate the diagnostic accuracy of urine-based lipoarabinomannan assay FUJIFILM SILVAMP TB LAM (FujiLAM) among inpatient and outpatient people living with HIV. Methods Diagnostic performance FujiLAM at point care was assessed adult HIV against a mycobacterial reference standard (sputum...
ABSTRACT Non-tuberculosis mycobacteria (NTM), particularly Mycobacterium abscessus subsp. ( M. ), are increasingly being recognized as etiological agents of NTM pulmonary disease. However, treatment options for limited owing to their natural resistance most antibiotics, including β-lactams. produces a class A β-lactamase, whose activity is inhibited by cyclic boronic acid β-lactamase inhibitors. We aimed evaluate the in vitro effects xeruborbactam, inhibitor, against when combined with five...
We determined MICs for, confirmed the presence of pncA mutations in, and performed pyrazinamidase testing on colonies (subclones) obtained from seven isolates that exhibited differential pyrazinamide (PZA) susceptibility. Six strains were found to exhibit characteristics resulting mixture possessing different properties. In addition, our analysis revealed large pncA-spanning deletions (1,565 bp, 4,475 6,258 bp) in three showed high PZA resistance.
Mycobacterium abscessus is a rapidly growing mycobacterium found mainly in patients with respiratory or cutaneous infections, but it rarely causes disseminated infections. Little known about the clinical characteristics, treatment, and prognosis of M infection. A 75-year-old Japanese woman who had been treated for 17 years corticosteroid antisynthetase syndrome antithreonyl-tRNA synthetase antibody developed swelling her right elbow. X-ray elbow joint showed osteolysis, magnetic resonance...
Recent clinical studies have revealed that reappearance of the same nontuberculous mycobacterium (NTM) infection is common after successful standard treatment [1, 2]. Using pulsed-field gel electrophoresis analysis, Wallace et al . [1] found ∼75% Mycobacterium avium - intracellulare complex (MAC) isolates identified are result reinfection. According to a recent study conducted by Koh [2] using repetitive sequence-based PCR all re-identified M. abscessus subsp. had unique genotype. Therefore,...
A 68-year-old man on peritoneal dialysis (PD) was hospitalized with the clinical picture of peritonitis. The patient diagnosed peritonitis caused by nontuberculous mycobacteria (NTM) according to positive Ziehl-Neelsen staining and negative Mycobacterium tuberculosis polymerase chain reaction results. Oral levofloxacin clarithromycin, later intraperitoneal imipenem were started. According anti-NTM susceptibility test results, oral minocycline administered. treated for 6 months. He recovered...
Mycobacterium abscessus subsp. (M. abscessus) is a rapidly growing mycobacterium that resistant to most antibiotics. The number of patients with pulmonary disease caused by M. increasing in several regions, and therapy involves long-term antibiotic combination treatments, although no standard treatment regimen has been established.To examine candidate regimens for maintenance antimicrobial against measuring MIC using the three-drug chequerboard method.We evaluated drug susceptibility 70...
The PATHFAST TB LAM Ag assay is based on a chemiluminescent enzyme immunoassay to quantify lipoarabinomannan (LAM) in sputum within 1 h, and was developed as an alternative conventional culture methods for monitoring tuberculosis (TB) treatment. This study aimed evaluate the analytical performance initial clinical feasibility of using five