A5031307664- Biosensors and Analytical Detection
- Mycobacterium research and diagnosis
- Advanced biosensing and bioanalysis techniques
- Respiratory viral infections research
- Tuberculosis Research and Epidemiology
- Influenza Virus Research Studies
- Adipokines, Inflammation, and Metabolic Diseases
- SARS-CoV-2 detection and testing
- Animal Disease Management and Epidemiology
- Diabetes Management and Research
- Pancreatic function and diabetes
- Mass Spectrometry Techniques and Applications
- Odor and Emission Control Technologies
- Vitamin D Research Studies
- Single-cell and spatial transcriptomics
- SARS-CoV-2 and COVID-19 Research
- Immunodeficiency and Autoimmune Disorders
- Biomarkers in Disease Mechanisms
- Antimicrobial Peptides and Activities
- DNA and Nucleic Acid Chemistry
- Advanced Chemical Sensor Technologies
- Metabolism, Diabetes, and Cancer
- Animal Virus Infections Studies
- Pneumonia and Respiratory Infections
- Diagnosis and treatment of tuberculosis
Waseda University
2019-2020
Polymerase chain reaction (PCR)-based antigen tests are technically difficult, time-consuming, and expensive, may produce false negative results requiring follow-up confirmation with computed tomography. The global coronavirus disease 2019 (COVID-19) pandemic has increased the demand for accurate, easy-to-use, rapid, cost-effective clinical application. We propose a de novo test diagnosing COVID-19 using combination of sandwich enzyme-linked immunosorbent assay thio-nicotinamide adenine...
An ultrasensitive method for the determination of proteins is described that combines an enzyme-linked immunosorbent assay (ELISA) and a thionicotinamide-adenine dinucleotide (thio-NAD) cycling method. A sandwich using primary secondary antibody antigens employed in ELISA. androsterone derivative, 3α-hydroxysteroid, produced by hydrolysis 3α-hydroxysteroid 3-phosphate with alkaline phosphatase linked to antibody. This oxidized 3-ketosteroid dehydrogenase (3α-HSD) cofactor thio-NAD. By...
Nucleic acid amplification tests (NAATs) are widely used to diagnose tuberculosis (TB), but cannot discriminate live bacilli from dead bacilli. Live can be isolated by culture methods, this is time-consuming. We developed a de novo TB diagnostic method that detects only with high sensitivity within hours.
Objective The chronic kidney disease (CKD) is widely diagnosed on the basis of albuminuria and glomerular filtration rate. A more precise diagnosis CKD, however, requires assessment other factors. Urinary adiponectin recently attracted attention for CKD assessment, but evaluation difficult due to very low concentration urinary in normal subjects. Research design methods We developed an ultrasensitive ELISA coupled with thionicotinamide-adenine dinucleotide cycling detect trace amounts...
ObjectivesThis study examined Mycobacterium tuberculosis (MTB)-secreted MPT64 as a surrogate of bacterial viability for the diagnosis active pulmonary TB (PTB) and follow-up treatment.MethodsIn this proof-of-concept prospective study, 50 PTB patients in Tokyo metropolitan region, between 2017 2018, were consecutively included 30 healthy individuals also included. Each patient submitted sputum on days 0, 14 28 follow-up, each individual one sample. The following performed: smear microscopy,...
To reduce the window period between HIV-1 infection and ability to diagnose it, a fourth-generation immunoassay including detection of p24 antigen has been developed. However, because commercially available systems for this assay use special, high-cost instruments measure, example, chemiluminescence, it is performed only by diagnostics companies hub hospitals. overcome limitation, we applied an ultrasensitive ELISA coupled with thio-NAD cycling, which based on usual enzyme without special...
To minimize patient suffering, the smallest possible volume of blood should be collected for diagnosis and disease monitoring. When estimating insulin secretion capacity resistance to in diabetes mellitus (DM), increasing assay immunosensitivity would reduce sample required testing. Here we present an ultrasensitive ELISA coupled with thio-NAD cycling measure immunoreactive serum. Only 5 μL serum was testing, a limit detection (LOD) 10(-16) moles/assay. Additional recovery tests confirmed...
For the diagnosis of disease, ability to quantitatively detect trace amounts causal proteins from bacteria/viruses as biomarkers in patient specimens is highly desirable. Here we introduce a simple, rapid, and colorimetric assay de novo, ultrasensitive detection method. This consists sandwich enzyme-linked immunosorbent (ELISA) thionicotinamide-adenine dinucleotide (thio-NAD) cycling, forming an ELISA, which signal substrate (i.e., thio-NADH) accumulates triangular manner, accumulated...
Adiponectin is a hormone secreted from adipocytes, and it demonstrates antidiabetic, anti-atherosclerotic, antiobesity anti-inflammatory effects. However, the patterns of change in urinary adiponectin levels various diseases remain unknown, because only trace amounts are present urine. In study, we applied an ultrasensitive ELISA coupled with thio-NAD cycling to measure levels. Spikeand-recovery tests using urine confirmed reliability our ELISA. The limit detection for was 2.3×10(-19)...
Highly pathogenic avian influenza viruses (HPAIVs) of H5 subtype have persistently caused outbreaks in domestic poultry and wild birds worldwide sporadically infected humans. Rapid accurate diagnosis is one the key strategies for control HPAIVs. However, sensitivity HPAIVs has gradually reduced due to extensive antigenic variation during their evolution. Particularly, previously developed immunochromatographic kit viruses, Linjudge Flu A/H5, exhibits detection isolated recent years. In...
Many cases of human infection with the H7N9 virus have been detected in China since 2013. viruses are maintained chickens and transmitted to humans at live bird markets. During circulation birds, accumulated amino acid substitutions their hemagglutinin (HA), which resulted an antigenically change recent viruses. Here, we characterized 46 mouse monoclonal antibodies against HA prototype strain. 16 H7-HA-specific (mAbs) possessed hemagglutination inhibition (HI) neutralization activities by...
Since the spring of 2013, human infections with H7N9 viruses have been detected in China. Some these become highly pathogenic. Highly and low pathogenic avian influenza are currently co-circulating seasonal A H3N2 H1N1pdm09. Prompt identification isolation patients is one measure to prevent spread virus help a pandemic. The majority commercially available point-of-care rapid diagnostic kits can differentiate between B viruses, but cannot distinguish viruses. Accordingly, we developed kit...
We propose a new detection method for tuberculosis-specific protein, MPB64, obtained from active bacillus Calmette-Guérin (BCG) by heating. When BCG was included in solution at concentration >2.75 × 104 CFU/ml, our collecting MPB64 through heating combined with an immunochromatographic assay detected bacilli within 2.5 h. By contrast, culture test, which is the gold standard tuberculosis diagnosis, does not provide results between 1 week and 2 months. The rapid tests based on PCR have some...
We developed a new measurement method for formaldehyde emission and examined its accuracy procedure. In this paper, results of the experimental examinations are described. Through experiments, we have found that will be applicable as new, simple quick measurement. Major obtained from experiments follows; 1. Environmental factors, example, temperature, humidity other VOC levels affect very little. 2. The necessary time required is rather short, within 45 minutes, therefore considered easier...