A5010288441- Dermatology and Skin Diseases
- Liver physiology and pathology
- Immune Cell Function and Interaction
- Drug-Induced Hepatotoxicity and Protection
- Lymphoma Diagnosis and Treatment
- Pharmacogenetics and Drug Metabolism
- Vasculitis and related conditions
- Toxin Mechanisms and Immunotoxins
- Liver Disease Diagnosis and Treatment
- Cutaneous lymphoproliferative disorders research
- Skin and Cellular Biology Research
- Urinary Tract Infections Management
- Immune Response and Inflammation
- Antimicrobial Peptides and Activities
- Fungal Biology and Applications
- CAR-T cell therapy research
- Systemic Lupus Erythematosus Research
- Ion Channels and Receptors
- Gastrointestinal motility and disorders
- Barrier Structure and Function Studies
- Connexins and lens biology
- Cell Adhesion Molecules Research
- IL-33, ST2, and ILC Pathways
- Gut microbiota and health
- Reproductive System and Pregnancy
University Hospital Leipzig
2022-2025
Leipzig University
2016-2025
Karolinska Institutet
2021-2023
Karolinska University Hospital
2023
Azienda Ospedaliera Universitaria Integrata Verona
2022
University of Verona
2022
Saxon Institute for Applied Biotechnology
2022
Abstract Diabetes is known to increase susceptibility infections, partly due impaired granulocyte function and changes in the innate immunity. Here, we investigate effect of diabetes, high glucose on expression antimicrobial peptide, psoriasin putative consequences for E. coli urinary tract infection. Blood, urine, urine exfoliated cells from patients are studied. The influence insulin examined during hyperglycemic clamps individuals with prediabetes euglycemic hyperinsulinemic clamped type...
Background: A detailed understanding of the dynamic fate changes hepatocytes, hepatic stellate cells (HSC), Kupffer (KC), and liver sinusoidal endothelial (LSEC) is critical for studying (patho)physiology during disease progression. Current isolation methods often focus on single cell types, limiting utility in comprehensive research. Aim: To develop a novel, semi-automated protocol simultaneous hepatocytes non-parenchymal (NPCs), including HSC, KC, LSEC, from mouse human, with high yield,...
Cutaneous T-cell lymphomas (CTCL) are characterized by focal infiltration of malignant T cell clones in solitary skin lesions. Many CTCL patients experience an indolent disease, but some progress to advanced disease with high fatality. We hypothesized that natural killer (NK) cells participate local control tumor growth skin. Immunohistochemistry and flow cytometry analysis the density, localization, phenotype function NK twenty-nine fresh or formalin-fixed biopsies from twenty-four...
Introduction Hepatotoxicity induced by immunotherapeutics is an appearing cause for immune-mediated drug-induced liver injury. Such immuno-toxic mechanisms are difficult to assess using current preclinical models and the incidence too low detect in clinical trials. As hepatotoxicity a frequent reason post-authorisation drug withdrawal, there urgent need immuno-inflammatory vitro hepatotoxic potential of immuno-modulatory candidates. We developed several test systems based on recombinant...
Abstract Systemic lupus erythematosus (SLE) is a multi-organ inflammatory disease with kidney inflammation, nephritis (LN), being one of the most severe manifestations. Immune complex deposits, particularly in glomeruli, and T cells, B myeloid mainly extraglomerular localization, contribute to process. Natural killer (NK) cells have been suggested play role autoimmune diseases, but not investigated detail renal before. In this exploratory study, we performed first characterization NK cell...
Abstract Urinary tract infection frequently caused by E. coli is one of the most common bacterial infections. Increasing antibiotic resistance jeopardizes successful treatment and alternative strategies are therefore mandatory. Metformin, an oral antidiabetic drug, has been shown to activate macrophages in protection against certain infecting microorganisms. Since epithelial cells often form first line defense, we here investigated effect on uroepithelial during infection. Metformin...
Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) is an autoimmune disease involving autoreactivity to proteinase 3 (PR3) as demonstrated by presence of ANCAs. While autoantibodies are screened for diagnosis, autoreactive T cells and their features less well-studied. Here, we investigated PR3-specific CD4+T cell responses in patients with PR3-AAV, using a cohort 72 either active or inactive disease. Autoreactive producing interferon γ response protein stimulation were...
Abstract Infections are common in patients with diabetes. Moreover, increasing incidence of antibiotic resistance impedes the complete bacterial clearance and calls for alternative treatment strategies. Along antibacterial resistance, compromised host conditions create a favorable condition disease progression. In particular, cell junction proteins major importance as they contribute to tight barrier, protecting against invading pathogens. However, impact high glucose on has received little...
Abstract The development of in vitro hepatocyte cell culture systems is crucial for investigating drug-induced liver injury (DILI). One prerequisite monitoring DILI related immunologic reactions the extension primary human (PHH) cultures towards inclusion macrophages. Therefore, we developed and characterized an autologous co-culture system PHH hepatic macrophages (hepM) (CoC1). We compared CoC1 with a same batch + M0 derived from THP1 cells (CoC2) order to represent donor independent...
Abstract Background To avoid the overuse of antibiotics, non-steroidal anti-inflammatory drugs (NSAIDs), acting via cyclooxygenase (COX) inhibition, have been used to reduce pain and as an alternative treatment for uncomplicated urinary tract infections (UTIs). However, clinical studies evaluating NSAIDs versus antibiotics reported increased risk acute pyelonephritis. Therefore, we hypothesized that COX inhibition could compromise innate immune response contribute complications in patients...
Non-alcoholic fatty liver disease (NAFLD), characterized by lipid accumulation in the liver, is most common cause of diseases Western countries. NAFLD a major risk factor for developing hepatocellular carcinoma (HCC); however, vitro evaluation hepatic cancerogenesis fails due to lack models displaying proliferation hepatocytes. Originally designed overcome primary human hepatocyte (PHH) shortages, upcyte hepatocytes were engineered obtain continuous and, therefore, could be suitable tool HCC...