A5029659313- Antimicrobial Peptides and Activities
- Urinary Tract Infections Management
- Nanoparticles: synthesis and applications
- Tuberculosis Research and Epidemiology
- Antimicrobial agents and applications
- Gut microbiota and health
- Immune Response and Inflammation
- Pediatric health and respiratory diseases
- Biochemical and Structural Characterization
- Urinary Bladder and Prostate Research
- Mycobacterium research and diagnosis
- Probiotics and Fermented Foods
- Coronary Interventions and Diagnostics
- Hydrogels: synthesis, properties, applications
- Acute Myocardial Infarction Research
- Graphene and Nanomaterials Applications
- Cardiovascular Syncope and Autonomic Disorders
- Reproductive tract infections research
- Cardiac Arrhythmias and Treatments
- Antibiotic Resistance in Bacteria
- Escherichia coli research studies
- Wound Healing and Treatments
- Advancements in Transdermal Drug Delivery
- Agricultural Economics and Practices
- Ion Channels and Receptors
Karolinska University Hospital
2017-2024
Karolinska Institutet
2017-2024
National Institute of Biomedical Genomics
2024
Czech Academy of Sciences, Institute of Microbiology
2024
Uppsala University
2023
INHS Asvini
2020-2021
Post Graduate Institute of Medical Education and Research
2021
KIIT University
2011-2015
College of New Jersey
2011
Background: Pathogenic bacteria are able to develop various strategies counteract the bactericidal action of antibiotics. Silver nanoparticles (AgNPs) have emerged as a potential alternative conventional antibiotics because their potent antimicrobial properties. The purpose this study was synthesize chitosan-stabilized AgNPs (CS-AgNPs) and test for cytotoxic, genotoxic, macrophage cell uptake, antibacterial, antibiofilm activities. Methods: were synthesized using chitosan both stabilizing...
ABSTRACT With the emergence of multidrug-resistant mycobacterial strains, better therapeutic strategies are required for successful treatment infection. Although antimicrobial peptides (AMPs) and silver nanoparticles (AgNPs) becoming one popular antibacterial agents, their antimycobacterial potential is not fully evaluated. In this study, we synthesized biogenic-silver using bacterial, fungal, plant biomasses analyzed activities in combination with AMPs against mycobacteria. Mycobacterium...
Abstract Biocompatible and degradable dual‐delivery gel systems based on hyperbranched dendritic−linear−dendritic copolymers (HBDLDs) is herein conceptualized accomplished via thiol‐ene click chemistry. The elasticity of the hydrogels tunable by varying lengths PEG (2, 6, 10 kDa) or dry weight percentages (20, 30, 40 wt%), are found to range from 2–14.7 kPa, comparable human skin. co‐delivery antibiotics achieved, where hydrophilic drug novobiocin sodium salt (NB) entrapped within hydrogel,...
Abstract Diabetes is known to increase susceptibility infections, partly due impaired granulocyte function and changes in the innate immunity. Here, we investigate effect of diabetes, high glucose on expression antimicrobial peptide, psoriasin putative consequences for E. coli urinary tract infection. Blood, urine, urine exfoliated cells from patients are studied. The influence insulin examined during hyperglycemic clamps individuals with prediabetes euglycemic hyperinsulinemic clamped type...
Pathogenic mycobacteria reside in, and are in turn controlled by, macrophages. However, emerging data suggest that neutrophils also play a critical role innate immunity to tuberculosis, presumably by their different antibacterial granule proteins. In this study, we purified neutrophil azurophil specific granules systematically analyzed the antimycobacterial activity of some proteins against M. smegmatis, bovis-BCG tuberculosis H37Rv. Using gel overlay colony forming unit assays showed...
Artemisia nilagirica (Asteraceae) and Murraya koenigii (Rutaceae) are widely distributed in eastern region of India. Leaves plant used to treat cold cough by the local tribal population east is an edible previously reported have antibacterial activity. Pathogenic strains mycobacteria resistant most conventional antibiotics. Therefore, it imperative identify novel antimycobacterial molecules mycobacterial infection.In this study, ethanol, petroleum ether water extracts were tested for...
Abstract The increasing antibiotic resistance among uropathogenic bacteria warrants alternative therapeutic strategies. We demonstrate the potential of synthetic peptide CD4-PP, designed by dimerization and backbone cyclization shortest antimicrobial region human cathelicidin, LL-37. CD4-PP is active against clinical type strains common uropathogens Escherichia coli , Klebsiella pneumoniae Pseudomonas aeruginosa at concentrations substantially below cellular cytotoxic levels induced membrane...
Antibacterial activity of silver nanoparticles is often associated with toxicity to the host. We here report that noncytotoxic doses coated zinc oxide, Ag@ZnO, can stimulate proliferation and migration human keratinocytes, HaCaT, increased expression Ki67 vinculin at leading edge wounds. Interestingly, Ag@ZnO stimulates keratinocytes produce antimicrobial peptides hBD2 RNase7, promoting antibacterial against both extracellular intracellular Staphylococcus aureus isolated from Overall, these...
Abstract Tight junction proteins are pivotal to prevent bacterial invasion of the epithelial barrier. We here report that supplementation with vitamin D can strengthen urinary bladder lining. Vitamin deficient and sufficient mice were infected Escherichia coli (E. coli) transurethrally cause tract infection. In addition, biopsies obtained from postmenopausal women before after a 3-month period 25-hydroxyvitamin 3 (25D ) ex vivo E. . biopsies, infection, had no impact on tight proteins....
Infections caused by antibiotic-resistant bacteria are globally a major threat, leading to high mortality rates and increased economic burden. Novel treatment strategies therefore urgently needed healthcare providers protect people. Biomaterials that have inherent antibacterial properties do not require the use of antibiotics present an attractive feasible avenue achieve this goal. Herein, we demonstrate effect new class cationic hydrogels based on amino-functional hyperbranched...
BackgroundMedical devices face the challenge of microbial biofilm attached to surface. Ultimately, this may jeopardize function device and increase patient's risk infection. However, reliable methods prevent are lacking.AimTo investigate effect silicone oil-coated polypropylene plastic, used in a new automatic urinometer, on formation; furthermore, explore impact oil viscosity compare with polystyrene, another common medical plastic.MethodsCommon pathogens, including extended-spectrum beta...
Cardiovascular disease is the leading cause of death in India. Our aim to study clinical, epidemiological profile and in-hospital outcomes patients presenting with acute coronary syndrome.We did a prospective single center observational 1203 ACS tertiary referral North India over period one year (July 2018-June 2019).The mean age population was 58.4 ± 12.5 years. STEMI NSTE-ACS accounted for 69.9% 31.1% respectively. 62.1% our were from rural background. The median time hospital admission...
Objectives Recurrent vulvovaginal candidiasis (RVVC) causes significant morbidity. Candida albicans is the main pathogen associated with both sporadic and recurrent candidiasis. Due to unsatisfactory treatment effect, impact of chlorhexidine digluconate fluconazole alone or in combination on C. biofilm was investigated. Methods Vaginal isolates from 18 patients commensals 19 asymptomatic women were isolated by culture. Crystal violet, XTT colony forming unit assay used analyze effect growth...
In article number 2006453, Michael Malkoch and co-workers report a series of sophisticated nanogel encapsulated hydrogels for the controlled co-delivery both hydrophobic hydrophilic antibiotics. The hybrid match strength flexibility human skin cause enhanced proliferation cells in vitro. antibacterial hydrogel band aids, fabricated facile procedure, far outperform commercial aids.
MV140 is an inactivated whole-cell bacterial mucosal vaccine with proven clinical efficacy against recurrent urinary tract infections (UTIs). These are primarily caused by uropathogenic E. coli (UPEC) strains, which unique in their virulence factors and remarkably diverse. contains a non-UPEC strain, suggesting that it may induce immune response different UPEC-induced UTIs patients. To verify this, we experimentally evaluated the cellular humoral responses to UTI89, prototypical UPEC mice...
Abstract Infections are common in patients with diabetes, but increasing antibiotic resistance hampers successful bacterial clearance and calls for alternative treatment strategies. Hypoxia-inducible factor 1 (HIF-1) is known to influence the innate immune defense could therefore serve as a possible target. However, impact of high glucose on HIF-1 has received little attention merits closer investigation. Here, we show that higher levels proinflammatory cytokines CAMP , encoding...
Abstract Urinary tract infection frequently caused by E. coli is one of the most common bacterial infections. Increasing antibiotic resistance jeopardizes successful treatment and alternative strategies are therefore mandatory. Metformin, an oral antidiabetic drug, has been shown to activate macrophages in protection against certain infecting microorganisms. Since epithelial cells often form first line defense, we here investigated effect on uroepithelial during infection. Metformin...