A5043188465- Ion Channels and Receptors
- Cardiac, Anesthesia and Surgical Outcomes
- Hemodynamic Monitoring and Therapy
- Anesthesia and Sedative Agents
- Neurobiology and Insect Physiology Research
- Ion channel regulation and function
- Respiratory Support and Mechanisms
- Postharvest Quality and Shelf Life Management
- Pain Mechanisms and Treatments
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Neuroscience and Neuropharmacology Research
- Pituitary Gland Disorders and Treatments
- Ultrasound in Clinical Applications
- Sphingolipid Metabolism and Signaling
- Metabolism and Genetic Disorders
- Foreign Body Medical Cases
- Lipid Membrane Structure and Behavior
- Pharmaceutical Practices and Patient Outcomes
- Hyperglycemia and glycemic control in critically ill and hospitalized patients
- Anesthesia and Pain Management
- Biochemical Analysis and Sensing Techniques
- Protein Kinase Regulation and GTPase Signaling
- Cardiac Ischemia and Reperfusion
- Nitric Oxide and Endothelin Effects
- Cancer, Hypoxia, and Metabolism
Kyushu University Hospital
2011-2023
Kyushu University
2010-2020
KU Leuven
2007-2010
TRPA1 functions as an excitatory ionotropic receptor in sensory neurons. It was originally described a noxious cold-activated channel, but its cold sensitivity has been disputed later studies, and the contribution of to thermosensing is currently matter strong debate. Here, we provide several lines evidence establish that acts sensor vitro vivo. First, demonstrate heterologously expressed activated by Ca 2+ -independent store-independent manner; temperature-dependent gating mechanistically...
TRPA1 is a calcium-permeable nonselective cation transient receptor potential (TRP) channel that functions as an excitatory ionotropic in nociceptive neurons. robustly activated by pungent substances mustard oil, cinnamon, and garlic mediates the inflammatory actions of environmental irritants proalgesic agents. Here, we demonstrate bimodal sensitivity to menthol, widely used cooling agent known activator related cold TRPM8. In whole-cell single-channel recordings heterologously expressed...
Clotrimazole (CLT) is a widely used drug for the topical treatment of yeast infections skin, vagina, and mouth. Common side effects CLT application include irritation burning pain skin mucous membranes. Here, we provide evidence that transient receptor potential (TRP) channels in primary sensory neurons underlie these unwanted CLT. We found clinically relevant concentrations activate heterologously expressed TRPV1 TRPA1, two TRP act as receptors irritant chemical and/or thermal stimuli...
We have investigated the cellular mechanisms of mechanical stress-induced immediate responses in human umbilical vein endothelial cells (HUVECs). Hypotonic stress (HTS) induced ATP release, which evoked a Ca(2+) transient, followed by actin reorganization within few minutes, HUVECs. Disruption cytoskeleton did not suppress HTS-induced and inhibition ATP-mediated response affect reorganization, thereby indicating that these two are interrelated. release were also lysophosphatidic acid (LPA)....
Lysophosphatidic acid (LPA) is a bioactive phospholipid that released from activated platelets and affects contractile properties of airway smooth muscle cells. However, possible roles LPA on cell migration, one the initial events remodeling, are not clarified. This study aimed to examine effects migration actin fiber formation in bovine tracheal cells (BTSMCs). Random oriented migrations were examined with wound assay Boyden chamber assay, respectively. Cytosolic fibers stained...
We examined the effects of superoxide anion (O) generated by xanthine plus oxidase (X/XO) on intracellular Ca(2+) concentration ([Ca(2+)](i)) and muscle contractility in cultured bovine aortic smooth cells (BASMC). Cells were grown collagen-coated dish for measurement [Ca(2+)](i). Pretreatment with X/XO inhibited ATP-induced transient release-activated entry (CRAC) after thapsigargin-induced store depletion, both which reversed dismutase (SOD). In contrast, transients induced high-K(+)...
We herein present a case of intramuscular hematoma that developed after transversus abdominis plane block in patient undergoing cesarean delivery. The had HELLP syndrome (hemolysis, elevated liver enzymes, and low platelet count) preoperatively. Ultrasonography-guided was performed at the end surgery. Postoperatively, count antithrombin III level decreased, computed tomography revealed hematomas possibly were related to vascular injury potential disseminated intravascular coagulation. should...
The G protein-gated inwardly rectifying K+ (GIRK) channels play important signaling roles in the central and peripheral nervous systems. However, role of GIRK channel activation pain remains unknown mainly due to lack potent selective activators until recently. present study was designed determine effects mechanisms ML297, a GIRK1/2 activator, on nociception spinal cord by using behavioral studies whole-cell patch-clamp recordings from substantia gelatinosa (SG) neurons. Rats were prepared...
Postreperfusion syndrome (PRS) after portal vein reperfusion during liver transplantation (LT) has been reported to cause rapid hemodynamic changes and is associated with a prolonged postoperative hospital stay, renal failure, increased mortality. Although there are some reports on risk factors for PRS in brain-dead donor LT, few those living LT. Therefore, we retrospectively reviewed the contribute anesthetic management so as reduce LT.After approval by ethics committee of our institution,...
We have previously reported that both hypotonic stress (HTS) and lysophosphatidic acid (LPA) induce ATP release a transient reorganization of actin through sequential activation RhoA/Rho-kinase focal adhesion kinase F-actin (FAK)/paxillin in human umbilical cord vein endothelial cells (HUVECs). LPA is known to the RhoA via its specific receptors, but mechanisms by which HTS initiates these intracellular signals are not known. The present study aimed identify molecule(s) unique sensing and/or...
Surgical operations often induce acute hyperglycemia, which is known to affect endothelial functions. In this study, we examined the effects of propofol, a commonly used general anaesthetic, on bovine aortic cell (BAEC) dysfunction induced by glucose overload. 2 D-glucose overload (23 mM) an accumulation superoxide anion (O2-), assessed MCLA chemiluminescence, similar extent as that generated 233 microU ml(-1) xanthine oxidase (XO) and 100 micro M xanthine. Propofol inhibited with IC50 0.21...
Bleeding and carbon dioxide (CO2) gas embolism have been reported as serious complications associated with laparoscopic surgery. We present a case of cerebral infarction presumably caused by CO2 during hepatectomy.During liver resection, the end-tidal suddenly dropped from 40 to 21 mmHg. Simultaneously, ST elevation in lead II depression V5 electrocardiogram were observed. After improvement these electrocardiographic changes, surgery was continued. Postoperatively, incomplete paralysis right...
Background Ultiva® is commonly administered intravenously for analgesia during general anaesthesia and its main constituent remifentanil an ultra-short-acting μ-opioid receptor agonist. not approved epidural or intrathecal use in clinical practice. Previous studies have reported that provokes opioid-induced hyperalgesia by interacting with spinal dorsal horn neurons. contains glycine, inhibitory neurotransmitter but also N-methyl-D-aspartate co-activator. The presence of glycine the...