The complete amino acid sequences and the disulfide arrangements of two chains human haptoglobin 1-1 were established. alpha 1 beta contain 83 245 residues, respectively. Comparison primary structure with that chymotrypsinogen family serine proteases revealed a significant degree chemical similarity. probability was less than 10(-5) similarity chain to due chance. sequence is 29--33% identical bovine trypsin, chymotrypsin, porcine elastase, thrombin, or plasmin. activation peptide regions...

Abstract The prevalence of allergies and asthma among the world’s population has been steadily increasing due to environmental factors. It described that exposure ozone, diesel exhaust particles, or tobacco smoke exacerbates allergic inflammation in lungs. These oxidants increase levels cellular reactive oxygen species (ROS) induce mitochondrial dysfunction airway epithelium. In this study, we investigated involvement preexisting exacerbation inflammation. After oxidative insult induced by...

The human endonuclease III (hNTH1), a homolog of the <i>Escherichia coli</i> enzyme (Nth), is DNA glycosylase with abasic (apurinic/apyrimidinic (AP)) lyase activity and specifically cleaves oxidatively damaged pyrimidines in DNA. Its cDNA was cloned, full-length (304 amino acid residues) expressed as glutathione <i>S</i>-transferase fusion polypeptide <i>E. coli</i>. Purified wild-type protein two additional residues truncated deletion 22 at NH₂ terminus were equally active had...

Respiratory syncytial virus (RSV) is a major cause of lower respiratory tract infections in children, for which no specific treatment or vaccine currently available. We have previously shown that RSV induces reactive oxygen species cultured cells and oxidative injury the lungs experimentally infected mice. The mechanism(s) RSV-induced stress vivo not known.To measure changes lung antioxidant enzymes expression/activity activation NF-E2-related factor 2 (Nrf2), transcription regulates...

We sought to identify cells and cytokines in bronchoalveolar lavage (BAL) fluids that distinguish asthma from healthy control subjects those controlled uncontrolled asthma. Following informed consent, 36 human were recruited for this study. These included 11 subjects, 15 with FEV1≥80% predicted 10 FEV1 <80% predicted. BAL fluid was obtained all subjects. The numbers of different cell types the levels 48 measured these fluids. Compared patients had significantly more percentages eosinophils...

The completed sequence of the beta-chain cholera toxin (103 amino acid residues) was compared to beta-chains chorionic gonadotropin, thyrotropin, luteinizing, and follicle stimulating hormones. overall chemical similarity hormones not statistically different from random; however, a comparison first 40 residues glycoprotein revealed segment which significantly chemically similar. probability less than .003 that due chance.

ABSTRACT Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract infections in infant and elderly populations worldwide. Currently, there no efficacious vaccine or therapy available for RSV infection. The molecular mechanisms underlying RSV-induced acute airway disease associated long-term consequences remain largely unknown; however, experimental evidence suggests that lung inflammatory response plays a fundamental role outcome High-mobility group box 1 (HMGB1)...

Abstract GMP-140 is an integral membrane glycoprotein of apparent Mr = 140,000 located in secretory storage granules platelets and vascular endothelial cells. When these cells are activated, redistributes from the to plasma membrane. To gain insight into potential function GMP-140, we examined aspects its structure biosynthesis. The amino acid composition platelet revealed elevated numbers cystinyl (6.1%), prolinyl (7.2%), tryptophanyl (2.1%) residues. contained 28.8% carbohydrate by weight,...

Anionic glutathione S-transferases were purified from human lung and placenta. Chemical immunochemical characterization, including polyacrylamide-gel electrophoresis, gave strong evidence that the anionic placental enzymes are chemically similar, if not identical, proteins. The electrophoretic mobilities of both proteins identical in conventional alkaline gels as well containing sodium dodecyl sulphate. Gel filtration intact active enzyme established an Mr value 45000; however, with...

The complete amino acid sequence of the beta chain cholera enterotoxin was determined: NH2-Thr-Pro-Gln-Asn-Ile-Thr-Asp-Leu-Cys-Ala-Glu-Tyr-His-Asn-Thr-Gln-Ile-His-Thr-Leu-Asn-Asn-Lys-Ile-Phe-Ser-Tyr-Thr-Glu-Ser-Leu-Ala-Gly-Lys-Arg-Glu-Met-Ala-Ile-Ile-Thr-Phe-Lys-Asn-Gly-Ala-Thr-Phe-Gln-Val-Glu-Val-Pro-Gly-Ser-Gln-His-Ile-Asp-Ser-Gln-Lys-Lys-Ala-Ile-Glu-Arg-Met-Lys-Asn-Thr-Leu-Arg-Ile-Ala-Tyr-Leu-Thr-Glu-Ala-Lys-Val-Glu-Lys-Leu-Cys-Val-Trp-Asn-Asn-Lys-Thr-Pro-His-Ala-Ile-Ala-Ala-Ile-Ser-Met-...

A significant amount of host cellular annexin II was found to be associated with human cytomegalovirus isolated from cultured fibroblasts (approximately 1,160 molecules per virion). This composition established by four different analytical approaches that included (i) Western blot (immunoblot) analysis gradient-purified virions a monoclonal antibody specific for II, (ii) peptide mapping and sequence virus-associated proteins dissociated virus following EDTA treatment, (iii) electron...

The pneumovirus respiratory syncytial virus (RSV) is a leading cause of epidemic tract infection. Upon entry, RSV replicates in the epithelial cytoplasm, initiating compensatory changes cellular gene expression. In this study, we have investigated RSV-induced nuclear proteome A549 alveolar type II-like cells by high-resolution two-dimensional gel electrophoresis (2DE). Replicate 2D gels from uninfected and RSV-infected nuclei were compared for protein We identified 24 different proteins...

Abstract Allergic diseases have been increasing in industrialized countries. The environment is thought to both direct and indirect modulatory effects on disease pathogenesis, including alterating the allergenicity of pollens. Certain plant proteins known as pathogenesis-related appear be up-regulated by certain environmental conditions, pollutants, some emerged important allergens. Thus, prospect environmentally regulated expression plant-derived allergens becomes yet another potential...

Secretory component (SC) is an integral membrane glycoprotein of secretory epithelial cells which responsible for the specific transport polymeric Ig (PIg) to external mucosal surfaces. The ectoplasmic segment binds comprised five Ig-type domains. Chemically and enzymatically modified forms portion SC (FSC) were produced tested their ability bind PIgA PIgM. Deglycosylated FSC bound specifically PIg, indicating that N-linked carbohydrate moieties on are not required binding. Denatured,...

ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTMolecular basis for the three major forms of human serum vitamin D binding protein (group-specific component)Jisnuson Svasti, Alexander Kurosky, Ann Bennett, and Barbara H. BowmanCite this: Biochemistry 1979, 18, 8, 1611–1617Publication Date (Print):April 17, 1979Publication History Published online1 May 2002Published inissue 17 April 1979https://pubs.acs.org/doi/10.1021/bi00575a036https://doi.org/10.1021/bi00575a036research-articleACS...

Abstract To understand the spectrum of proteins affected by diabetes and to characterize molecular functions biological processes they control, we analyzed renal cortical proteome db/db mice using 2‐DE combined with MALDI‐TOF, MALDI‐TOF/TOF, LC‐MS/MS. This approach yielded 278 high confidence identifications whose expression levels were significantly increased or decreased &gt;two‐fold diabetes, which 170 mapped gene identifiers representing 147 nonredundant proteins. Gene Ontology...

Neutrophil recruitment is a hallmark of rapid innate immune responses. Exposure airways naive mice to pollens rapidly induces neutrophil recruitment. The mechanisms that regulate pollen-induced and the contribution this neutrophilic response subsequent induction allergic sensitization inflammation need be elucidated. Here we show ragweed pollen extract (RWPE) challenge in C-X-C motif ligand (CXCL) chemokine synthesis, which stimulates (C-X-C motif) receptor 2 (CXCR2)-dependent neutrophils...

Human cytomegalovirus (HCMV) stimulates arrested cells to enter the cell cycle by activating cyclin-dependent kinases (Cdks), notably Cdk2. Several mechanisms are involved in activation of HCMV causes a substantial increase abundance cyclin E and translocation Cdk2 from cytoplasm nucleus. Further, Cdk inhibitors (CKIs) p21cip1/waf1 (p21cip1) p27kip1 is substantially reduced. The activity E/Cdk2 increases as levels CKIs, particularly p21cip1, fall. We have previously shown that these...

The atrial gland of the marine mollusk Aplysia californica is an exocrine organ that expresses at least three genes belonging to egg-laying hormone (ELH) family. In order study post-translational processing ELH-related gene products in and how it compares bag cells, peptides were isolated from chemically characterized. A- B-related precursors each cleaved vivo yield several major minor including A B peptide complexes caused egg laying. About 13% further enzymically processed by smaller...