A5076015973- Asthma and respiratory diseases
- Allergic Rhinitis and Sensitization
- IL-33, ST2, and ILC Pathways
- Pediatric health and respiratory diseases
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Mast cells and histamine
- Respiratory and Cough-Related Research
- Dermatology and Skin Diseases
- DNA Repair Mechanisms
- Circadian rhythm and melatonin
- Antimicrobial Peptides and Activities
- Food Allergy and Anaphylaxis Research
- Contact Dermatitis and Allergies
- Neonatal Respiratory Health Research
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Eosinophilic Esophagitis
- Occupational exposure and asthma
- Eosinophilic Disorders and Syndromes
- Immunotherapy and Immune Responses
- Melanoma and MAPK Pathways
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cytokine Signaling Pathways and Interactions
- Ocular Surface and Contact Lens
- Bioactive Compounds and Antitumor Agents
Baylor College of Medicine
2020-2024
The University of Texas Medical Branch at Galveston
2011-2021
Galveston College
2004-2016
Hospital Universitario Infanta Leonor
2015
Hallym University
2015
Hospital Regional Universitario de Málaga
2015
Instituto de Investigación Biomédica de Málaga
2015
Philipps University of Marburg
2015
Universidad Complutense de Madrid
2015
Cells Therapy (Poland)
2015
To determine the histologic differences in airways of patients who died from sudden-onset asthma and more common slow-onset asthma, we studied seven cases fatal asthma. The numbers eosinophils neutrophils, as well extracellular deposition their respective granule contents airway mucosa submucosa, were determined statistically analyzed. Four had attacks which time interval between onset death was than 2.5 h. In contrast, three attack less 1 four with (34.1 +/- 6.3 slow-onset; 9.7 3.5...
Asthma is an inflammatory disease of the airways that induced by Th2 cytokines and inhibited Th1 cytokines. Despite a steady increase in incidence, morbidity, mortality from asthma, no current treatment can reduce or prevent asthma for prolonged period. We examined ability unmethylated CpG oligodeoxynucleotides (ODN), which are potent inducers cytokines, to physiological manifestations mice sensitized ragweed allergen. Administration ODN 48 h before allergen challenge increased ratio...
Pollen exposure induces allergic airway inflammation in sensitized subjects. The role of antigenic pollen proteins the induction is well characterized, but contribution other constituents grains to this process unknown. Here we show that and their extracts contain intrinsic NADPH oxidases. oxidases rapidly increased levels ROS lung epithelium as amount oxidized glutathione (GSSG) 4-hydroxynonenal (4-HNE) airway-lining fluid. These oxidases, products oxidative stress (such GSSG 4-HNE)...
Abstract The prevalence of allergies and asthma among the world’s population has been steadily increasing due to environmental factors. It described that exposure ozone, diesel exhaust particles, or tobacco smoke exacerbates allergic inflammation in lungs. These oxidants increase levels cellular reactive oxygen species (ROS) induce mitochondrial dysfunction airway epithelium. In this study, we investigated involvement preexisting exacerbation inflammation. After oxidative insult induced by...
We investigated the effects of IFN-gamma-inducing factor (IL-18) in a ragweed (RW) mouse model allergic asthma. Administration IL-18 conjunction with sensitization and challenge wild-type, but not IFN-gamma -/- mice, inhibited bronchoalveolar lavage (BAL) eosinophilia induced by RW challenge, increased serum levels RW-specific IgG2a production from splenocytes cultured RW, indicating critical role for mediating these effects. Paradoxically, same treatment schedule WT mice IgE IgG1, IL-4 IL-5...
The mechanism of airway eosinophilia during antigen-induced inflammation was investigated by measurement eosinophil-active cytokines utilizing an eosinophil survival assay. In the first study, 4 patients with allergic rhinitis underwent segmental bronchoprovocation (SBP) low, medium, and high doses ragweed extract instilled into different bronchial subsegments; bronchoalveolar lavage (BAL) fluids were collected from each segment 12 min 48 h after challenge. Eosinophil granule proteins...
8-Oxo-7,8-dihydroguanine (8-oxoG), arguably the most abundant base lesion induced in mammalian genomes by reactive oxygen species, is repaired via excision repair pathway that initiated with of 8-oxoG OGG1. Here we show OGG1 binds high affinity and complex then interacts canonical Ras family GTPases to catalyze replacement GDP GTP, thus serving as a guanine nuclear exchange factor. OGG1-mediated activation leads phosphorylation mitogen-activated kinases MEK1,2/ERK1,2 increasing downstream...
We sought to identify cells and cytokines in bronchoalveolar lavage (BAL) fluids that distinguish asthma from healthy control subjects those controlled uncontrolled asthma. Following informed consent, 36 human were recruited for this study. These included 11 subjects, 15 with FEV1≥80% predicted 10 FEV1 <80% predicted. BAL fluid was obtained all subjects. The numbers of different cell types the levels 48 measured these fluids. Compared patients had significantly more percentages eosinophils...
We investigated the effects of IL-12 on a murine model allergic lung inflammation. Administration was timed to interfere with either sensitization (early dosage) or hypersensitivity inflammatory response in (late dosage), both and late dosages). Comparisons IL-12- PBS-treated animals within each treatment group revealed several noticeable IL-12. Early dosage, combination early dosages, strikingly decreased ragweed-specific serum IgE, tracheal ring reactivity acetylcholine, BAL eosinophilia...
We investigated the effects of interleukin (IL)-10 administration on allergen-induced Th2 cytokine production, eosinophilic inflammation, and airway reactivity. Mice were sensitized by intraperitoneal injection ragweed (RW) adsorbed to Alum challenged intratracheal instillation allergen. Sensitization challenge with RW increased concentrations IL-10 in bronchoalveolar lavage (BAL) fluid from undetectable levels 60 pg/ml over 72 h. Intratracheal 25 ng recombinant murine at time further...
Abstract Eosinophil-derived neurotoxin (EDN) and eosinophil cationic protein (ECP) are generally regarded as eosinophil-specific proteins. We tested whether EDN ECP present in mature neutrophils. By indirect immunofluorescence, both eosinophils neutrophils stained with antibodies to ECP. Lysates of purified (&lt;0.1% contamination) contained EDN, 112 ± 4 ng/106 cells, ECP, 163 2 whereas major basic (MBP) was not detectable. Electron microscopic examination immunogold-labeled buffy coat...
Pollen grains contain reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidases and in contact with mucosal surfaces generate superoxide anion (O2*-). In the presence of iron, O2*- may be converted to more reactive oxygen radicals, such as H2O2 and/or *OH, which augment antigen-induced airway inflammation. The aim study was examine impact lactoferrin (LF), an iron-binding protein, on ragweed (Ambrosia artemisiifolia) pollen extract (RWE)-induced cellular oxidative stress levels...
Why mammalian cells possess multiple DNA glycosylases (DGs) with overlapping substrate ranges for repairing oxidatively damaged bases via the base excision repair (BER) pathway is a long-standing question. To determine biological role of these DGs, null animal models have been generated. Here, we report generation and characterization mice lacking Neil2 (Nei-like 2). As in deficient each other four oxidized base-specific DGs (OGG1, NTH1, NEIL1, NEIL3), Neil2-null show no overt phenotype....
Abstract 8-Oxoguanine-DNA glycosylase-1 (OGG1) is the primary enzyme for repairing 7,8-dihydro-8-oxoguanine (8-oxoG) via DNA base excision repair pathway (OGG1-BER). Accumulation of 8-oxoG in genomic leads to genetic instability and carcinogenesis thought contribute worsening various inflammatory disease processes. However, mechanism unknown. In this study, we proposed that mechanistic link between OGG1-BER proinflammatory gene expression OGG1’s guanine nucleotide exchange factor activity,...
Oxidative stress from ozone (O(3)) exposure augments airway neutrophil recruitment and chemokine production. We others have shown that severe sudden asthma is associated with neutrophilia, O(3) oxidative likely to augment neutrophilic inflammation in asthma. However, very little known about chemokines orchestrate stress-induced vivo. To identify these chemokines, three groups of BALB/c mice were exposed sham air, 0.2 ppm O(3), or 0.8 for 6 h. Compared but not induced pronounced peaked at 18...