A5100384057- RNA Research and Splicing
- Cancer-related molecular mechanisms research
- Breast Cancer Treatment Studies
- RNA modifications and cancer
- Carcinogens and Genotoxicity Assessment
- Circular RNAs in diseases
- HER2/EGFR in Cancer Research
- Estrogen and related hormone effects
- MicroRNA in disease regulation
- Breast Implant and Reconstruction
- Breast Lesions and Carcinomas
- Plant-based Medicinal Research
- Ferroptosis and cancer prognosis
- RNA Interference and Gene Delivery
- Plant Toxicity and Pharmacological Properties
- Immunotoxicology and immune responses
- Cancer, Hypoxia, and Metabolism
- Peroxisome Proliferator-Activated Receptors
- Phytase and its Applications
- Plant Reproductive Biology
- Neurogenesis and neuroplasticity mechanisms
- Genomic variations and chromosomal abnormalities
- Immunodeficiency and Autoimmune Disorders
- Bioactive natural compounds
- Sulfur Compounds in Biology
Kangwon National University
2024
Inje University Sanggye Paik Hospital
2005-2022
University of Copenhagen
2018
Korea Institute of Toxicology
2007-2018
National Institute on Aging
2013-2017
National Institutes of Health
2013-2017
Institute on Aging
2015-2017
University of Baltimore
2015
Incheon St. Mary's Hospital
2015
Catholic University of Korea
2015
HuR influences gene expression programs and hence cellular phenotypes by binding to hundreds of coding noncoding linear RNAs. However, whether binds circular RNAs (circRNAs) impacts on their function is unknown. Here, we have identified en masse circRNAs in human cervical carcinoma HeLa cells. One the most prominent target was hsa_circ_0031288, renamed CircPABPN1 as it arises from PABPN1 pre-mRNA. Further analysis revealed that did not influence abundance; interestingly, however, high levels...
Some mitochondrial long noncoding RNAs (lncRNAs) are encoded by nuclear DNA, but the mechanisms that mediate their transport to mitochondria poorly characterized. Using affinity RNA pull-down followed mass spectrometry analysis, we found two RNA-binding proteins (RBPs), HuR (human antigen R) and GRSF1 (G-rich sequence-binding factor 1), associated with DNA-encoded lncRNA RMRP mobilized it mitochondria. In cultured human cells, bound in nucleus mediated its CRM1 (chromosome region maintenance...
The function of the vast majority mammalian long noncoding (lnc) RNAs remains unknown. Here, analysis a highly abundant lncRNA, OIP5-AS1, known as cyrano in zebrafish, revealed that OIP5-AS1 reduces cell proliferation. In human cervical carcinoma HeLa cells, RNA-binding protein HuR, which enhances proliferation, associated with and stabilized it. Tagging MS2 hairpins to identify microRNAs miR-424 interacted competed HuR for binding OIP5-AS1. We further identified 'sponge' high levels...
Abstract Noncoding RNAs (ncRNAs) and RNA-binding proteins are potent post-transcriptional regulators of gene expression. The ncRNA 7SL is upregulated in cancer cells, but its impact upon the phenotype cells unknown. Here, we present evidence that forms a partial hybrid with 3′-untranslated region (UTR) TP53 mRNA, which encodes tumor suppressor p53. interaction mRNA reduced p53 translation, as determined by analyzing expression levels, nascent translation association polysomes. Silencing led...
The levels of microRNAs (miRNAs) are altered under different conditions such as cancer, senescence, and aging. Here, we have identified differentially expressed miRNAs in skeletal muscle from young old rhesus monkeys using RNA sequencing. In muscle, several were upregulated, including miR-451, miR-144, miR-18a miR-15a, while a few downregulated, miR-181a miR-181b. A number novel also identified, particularly muscle. We examined the impact caloric restriction (CR) on miRNA abundance by...
Aging | doi:10.18632/aging.100834. Kotb Abdelmohsen, Amaresh C. Panda, Supriyo De, Ioannis Grammatikakis, Jiyoung Kim, Jun Ding, Ji Heon Noh, Kyoung Mi Julie A. Mattison, Rafael de Cabo, Myriam Gorospe
Highlights•A splice variant of TRF2 with a short exon 7 (TRF2-S) arises during neurogenesis•HNRNPH1/H2 binds Trf2 and represses splicing into TRF2-S•During neurogenesis, HNRNPH1/H2 levels decline while TRF2-S increase•Silencing elevates promotes neurogenesisSummaryDuring neuronal differentiation, use an alternative site on the rat telomere repeat-binding factor 2 (TRF2) mRNA generates protein isoform capable derepressing genes. However, RNA-binding proteins (RBPs) controlling this event are...
Vascular endothelial growth factor (VEGF) A is a master regulator of neovascularization and angiogenesis. VEGFA potently induced by hypoxia pathological conditions including diabetic retinopathy tumorigenesis. Fine-tuning expression different stimuli important for maintaining tissue vascularization organ homeostasis. Here, we tested the effect mimetic cobalt chloride (CoCl2) on in human cervical carcinoma HeLa cells. We found that CoCl2 increased levels mRNA protein without affecting...
// Jiyoung Kim 1 , Ji Heon Noh Seung-Kyu Lee Rachel Munk Alexei Sharov Elin Lehrmann Yongqing Zhang Weidong Wang Kotb Abdelmohsen and Myriam Gorospe Laboratory of Genetics Genomics, National Institute on Aging-Intramural Research Program, NIH, Baltimore, MD 21224, USA Correspondence to: Kim, email: kim.jiyoung@elixirgen.com Gorospe, myriam-gorospe@nih.gov Keywords: lncRNA, proliferation, mitosis Received: January 22, 2017 Accepted: March Published: April 19, ABSTRACT Some long noncoding RNAs...
Guibi-Tang is a traditional herbal prescription made from 12 different herbs that used in the treatment of amnesia and poor memory. In present study, we evaluated acute oral toxicity genotoxic potential water extract (GBT) at doses up to 2000 μg/plate an using bacterial reverse mutation test (Ames test) with Salmonella typhimurium strains TA100, TA1535, TA98, TA1537, Escherichia coli strain WP2uvrA. Acute were measured presence absence exogenous source metabolic activation, vitro chromosome...
Background One of the two copies X chromosome is randomly inactivated in females as a means dosage compensation. Loss inactivation (XCI) observed breast and ovarian cancers, frequent basal-like subtype BRCA1 mutation-associated cancers. We investigated clinical implications loss XCI cancer association between dysfunction. Materials Methods used open source data generated by The Cancer Genome Atlas (TCGA) Data Analysis Centers. Ward's hierarchical clustering method was to classify methylation...
To investigate the efficacy and safety of accelerated partial breast irradiation (APBI) via high-dose-rate (HDR) multicatheter interstitial brachytherapy for early-stage cancer. Between 2002 2006, 48 prospectively selected patients with cancer received APBI using following breast-conserving surgery. Their median age was 52 years (range 36-78). A 34 Gy 30-34) in 10 fractions given twice daily within 5 days delivered to tumor bed plus a 1-2 cm margin. Most (92%) adjuvant systemic treatments....
The luminal subtype of breast cancer is sensitive to anti-estrogen therapy and shows a better prognosis than that human epidermal growth factor receptor2 (HER2)-enriched or triple-negative cancer. However, the type heterogeneous can have aggressive clinical features. We investigated implications single hormone receptor negativity in B HER2-negative group.We collected data were estrogen (ER) and/or progesterone (PR) positive, Ki 67 high (>14 %), HER2 negative divided them into ER- PR-positive...
Gyejibokryeong-hwan (Guizhi Fuling Wan in China), a mixture of five herbal plants, is well-known treatment for renal diseases including those associated with climacteric syndrome. However, the genotoxicity has not yet been well established.The present study investigated that an aqueous extract (GJBRHE): vitro chromosomal aberration test using Chinese hamster lung cells, bacterial reverse mutation assay (Ames test) Salmonella typhimurium and Escherichia coli strains, vivo micronucleus ICR...
Oryeong-san, a mixture of five herbal plants, is well-known therapy for renal-associated diseases such as those manifesting edema, dysuria, and oliguria. In the present study, we investigatee potential genotoxic effects water extract Oryeong-san (ORSE) in three mutagenicity assays (an vitro bacterial reverse mutation assay (Ames test) with Salmonella typhimurium Escherichia coli strains, an mammalian chromosomal aberration test using Chinese hamster lung cells, vivo micronucleus ICR mice...
The suppression subtractive hybridization (SSH) method was used to isolate differentially expressed genes during carnation flower maturation. Five cDNA clones, designated as maturation-induced (CFMI), were verified cDNAs. Sequence analysis of five CFMI (CFMI-5, CFMI-6, CFMI-7, CFMI-9, and CFMI-10) clones revealed that one the CFMI-5, showed high sequence similarity cysteine proteinase inhibitor gene, predicted be involved in full length clone CFMI-5 531 nucleotides (nts) long consisted an...
Mannasantin B, a dilignan structurally related to manssantin A, is an inhibitor of NF-κB transactivation. In the present study, we found that it inhibited PMA-induced expression IL-1β, IL-1β mRNA, and promoter activity in U937 cells with IC50 values about 50 nM. It also NF-IL6- NF-κB-induced activation 78 nM 1.6 μM, respectively, revealing potent inhibitory effect on NF-IL6. Electrophoretic mobility shift assays showed manassantin B had DNA binding by NF-IL6, but not NF-κB. Further analysis...