Cirrhotic patients with gastroesophageal varices and non-tumoral portal vein thrombosis have a higher risk of re-bleeding poor prognosis. This study aimed to analyze inflammatory biomarkers thromboelastography in cirrhotic thrombosis.A total 385 consecutive were prospectively enrolled between 1 December 2016, 31 August 2017. Of these, 231 eligible for analysis divided into (n = 103) non-portal 128) groups based on computerized tomography angiography findings.Patients generally had Child-Pugh...

Pulmonary endothelial barrier dysfunction is a hallmark of clinical pulmonary edema and contributes to the development acute lung injury (ALI). Here we reported that ruscogenin (RUS), an effective steroidal sapogenin Radix Ophiopogon japonicus, attenuated lipopolysaccharides (LPS)-induced disruption through mediating non-muscle myosin heavy chain IIA (NMMHC IIA)‒Toll-like receptor 4 (TLR4) interactions. By in vivo vitro experiments, observed RUS administration significantly ameliorated...

BACKGROUND: Acute myocardial infarction (MI) remains one of the major causes death worldwide, and innovative treatment strategies for MI represent a challenge in cardiovascular medicine. Caloric restriction (CR) is most potent nonpharmacological intervention known to prevent age-related disorders extend lifespan. CR reduces glycolysis elevates ketone body metabolism. However, whether how or prevents progression poorly defined. METHODS: Mice treated with β-hydroxybutyrate (β-OHB) underwent...

Summary Tomato stress‐associated proteins (SAPs) belong to A20/AN1 zinc finger protein family, some of which have been shown play important roles in plant stress responses. However, little is known about the functions and underlying molecular mechanisms SAPs immune In present study, we reported function tomato SlSAP3 immunity Pseudomonas syringae pv. ( Pst ) DC3000. Silencing attenuated while overexpression transgenic increased DC3000, accompanied with reduced DC3000‐induced expression SA...

Abstract Long noncoding RNAs (LncRNAs) have been gaining attention as potential therapeutic targets for lung cancer. In this study, we investigated the expression and biological behavior of lncRNA DARS‐AS1, its predicted interacting partner miR‐302a‐3p, ACAT1 in nonsmall cell cancer (NSCLC). The transcript level was analyzed using qRT‐PCR. Endogenous of—and changes in—AKT/ERK pathway‐related proteins were determined western blotting. MTS, Transwell, apoptosis experiments used to investigate...

Acute lung injury (ALI) and acute respiratory distress syndrome (ARDS) are the serious diseases that characterized by a severe inflammatory response of injuries damage to microvascular permeability, frequently resulting in death. YiQiFuMai (YQFM) lyophilized injection powder is redeveloped preparation based on well-known traditional Chinese medicine formula Sheng-Mai-San which widely used clinical practice China, mainly for treatment microcirculatory disturbance-related diseases. However,...

PD-L1 is a critical checkpoint that protects tissues from autoimmune injury. Nevertheless, the role of in nonalcoholic fatty liver disease- (NAFLD-) induced damage still unclear. In this study, we examined and mechanism expression on NAFLD-induced vitro vivo. livers patients with NAFLD, LO2 cells treated by FFA, was significantly increased. FFA triggers large amount ROS (generated NOX4 damaged mitochondria), promoting ZNF24 suppressing ZN24 sumoylation, both which enhance transcription...

Abstract The exostosin (EXT) protein family is involved in diverse human diseases. However, the expression and prognostic value of EXT genes lung squamous cell carcinoma (LUSC) not well understood. In this study, we analyzed association between EXT1 EXT2 survival patients with LUSC using bioinformatics resources such as Oncomine Cancer Genome Atlas (TCGA) databases, Gene Expression Profiling Interactive Analysis (GEPIA) server Kaplan–Meier plotter. Furthermore, regulatory microRNAs (miRNAs)...

Background : Esophagogastric variceal bleeding (EVB) is a common and ominous complication of cirrhosis represents the degree portal hypertension progression decompensation, desiderating investigation into sensitive specific markers for early detection prediction. The purpose this study to characterize unique metabolites in serum cirrhotic EVB patients identify potential noninvasive biomarkers detecting assessing risk through metabolomics-based approaches explore possible pathophysiological...

Osteocalcin (Ocn), also known as bone Gla protein, is synthesized by osteoblasts and thought to regulate energy metabolism, testosterone synthesis brain development. However, its function in not fully understood. Mice have three Ocn genes: Bglap, Bglap2 Bglap3. Due the long span of these genes mouse genome low expression Bglap3 bone, researchers commonly use Bglap knockout mice investigate Ocn. it unclear whether has any compensatory mechanisms when are knocked out. Considering controversy...

Abstract RUNX1, a member of the RUNX family metazoan transcription factors, participates in regulation differentiation, proliferation, and other processes involved growth development. It also functions occurrence development tumors. However, role mechanism action RUNX1 non‐small cell lung cancer (NSCLC) are not yet clear. We used bioinformatics approach as well vitro vivo assays to evaluate NSCLC molecular mechanisms underlying its effects. Using TCGA, GEO, GEPIA (Gene Expression Profiling...

Portal vein thrombosis (PVT) is a challenging and controversial complication of cirrhosis. Experimental models that reproduce cirrhotic PVT effective pharmacological therapies are limited. We aimed to investigate the nature course mechanisms in A novel model was developed via two-step total portal ligation healthy thioacetamide (TAA)-cirrhotic rats. Circulating liver-infiltrating neutrophils were isolated from individuals with cirrhosis examine neutrophil extracellular traps (NETs) explore...

Rationale: Both bulk RNA-sequencing and GEO database upon chemotherapy to non-small cell lung cancer (NSCLC) cells reveal that ZNF131 (Zinc Finger Protein 131) maybe a crucial transcriptional factor involved. However, it is recently discovered protein with largely unexplored expression patterns biological functions. Methods: Bioinformatics analyses immunohistochemistry staining were assessed detect both mRNA levels of in NSCLC specimens lines. Next, colony formation assay, MTT EdU transwell...

Portal hypertension is a common clinical symptom of digestive disorders. With an increase in portal pressure, the vein will continue to dilate. We aimed determine whether continuous stretch induced by may impair function endothelial cells (ECs) and aggravate progress explore its mechanism. ECs were cultured on elastic silicone membrane subjected uniaxial stretch. Apoptosis expression TGF- β under measured. found that sustained apoptosis length-dependent manner. Compared with control,...

Background: GDF15 is a potential biomarker for patients with esophageal cancer (EC). However, the mechanistic role of in invasion and metastasis EC remains poorly understood. Methods: We determined expression function cells (ESCCs) patient tissue samples using western blotting, migration, assays, immunohistochemistry, Co-IP quantitative real-time-PCR. In addition, pulmonary metastatic nude mouse model was used to determine GDF15. then supplemented our experimental results database analysis...

Major depressive disorder (MDD) has a high global incidence. While the pathogenesis of depression remains unknown, accumulating evidence implicated inflammatory changes.The aim study is to compare serum complement C1q levels in patients with MDD and healthy controls.The design was case-control study.Blood samples were collected from controls assess using an immunotransmission turbidimetric method.Differences between controls, as well sexes among assessed Mann-Whitney U-test. Spearman...

Studying the relationship between vegetation structure and diversity is important in an area having karst topography unique traditional customs. We selected a total of six villages Zunyi City, China, to collect data. Additionally, using one-way ANOVA Pearson correlation coefficient analytic method analyze, results showed that, overall, plant communities were mostly regularly distributed. The overall differentiation degree was low aggregation, intensity, extreme intensity mixed state....

Background The aim of this study was to identify the role factor VIII (FVIII) in portal vein thrombosis (PVT) occurrence cirrhotic patients with gastroesophageal variceal bleeding. Methods A total 453 varices were enrolled. Computed tomography performed at baseline and divided into PVT non-PVT groups (n = 131 vs. 322). Individuals without followed up for development PVT. Time-dependent receiver operating characteristic analysis FVIII performed. Kaplan–Meier methodology used analyze...

The novel weapon hypothesis suggests that allelopathy is an important mechanism for exotic plants to successfully invade native plant communities. Allelochemicals from affect both and arbuscular mycorrhizal fungi (AMF) in soil. To explore these effects, we conducted pot experiments using a community comprising of Chenopodium album L., Vitex negundo Rhus chinensis Mill., Acer truncatum Bunge. We incorporated AMF strains ( Funneliformis mosseae , Glomus versiforme Rhizophagus intraradices...

Abstract Background Oxaliplatin, used as first-choice treatment for colorectal cancer (CRC), induces sinusoidal endothelial injury and portal hypertension. This study investigated the characteristics of oxaliplatin-induced hypertension evaluated efficacy endoscopic management gastroesophageal variceal bleeding. Methods We performed a retrospective, multicenter, case-control between January 2010 December 2018. Patients who received oxaliplatin-based chemotherapy after CRC surgery presented...

Portal vein thrombosis (PVT) is a serious complication of cirrhosis accompanied by unclear pathogenesis. Transforming growth factor-beta (TGF-β) has been implicated in atherosclerosis and venous whereas study regarding its part PVT lacking. The aim this was to explore the role cytokine TGF-β1 potential mechanism.We included patients with cirrhotic gastroesophageal varices divided them into two groups according presence PVT. Serum levels were detected using Cytometric Bead Array kit compared...