A5100745115- Advances in Cucurbitaceae Research
- Cancer-related molecular mechanisms research
- RNA modifications and cancer
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Genomics and Phylogenetic Studies
- Plant Virus Research Studies
- RNA Research and Splicing
- Molecular Biology Techniques and Applications
- Cocoa and Sweet Potato Agronomy
- Photosynthetic Processes and Mechanisms
- Cancer-related gene regulation
- Nematode management and characterization studies
- Legume Nitrogen Fixing Symbiosis
- Natural product bioactivities and synthesis
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Plant biochemistry and biosynthesis
- Retinoids in leukemia and cellular processes
- Agronomic Practices and Intercropping Systems
- Protein Tyrosine Phosphatases
- Cell death mechanisms and regulation
- Plant Gene Expression Analysis
- Inflammasome and immune disorders
- Inflammatory Bowel Disease
Nanjing Agricultural University
2015-2024
Central South University
2008-2024
Xiangya Hospital Central South University
2024
Peking Union Medical College Hospital
2013-2024
Chinese Academy of Medical Sciences & Peking Union Medical College
2013-2024
China Medical University
2020-2024
First Hospital of China Medical University
2024
Henan Agricultural University
2022-2023
Shandong Tumor Hospital
2020-2022
Renmin Hospital of Wuhan University
2020-2022
Exposure of macrophages to LPS elicits the production proinflammatory cytokines, such as TNF-alpha, through complex signaling mechanisms. Mitogen-activated protein (MAP) kinases play a critical role in this process. In present study, we have addressed MAP kinase phosphatase-1 (MKP-1) regulating cytokine using RAW264.7 macrophages. Analysis activity revealed transient activation c-Jun N-terminal (JNK) and p38 after stimulation. Interestingly, MKP-1 was induced concurrently with inactivation...
Alternative splicing of mRNA precursors represents a key gene expression regulatory step and permits the generation distinct protein products with diverse functions. In genome-scale screen for inducers epithelial-to-mesenchymal transition (EMT), we found striking enrichment RNA-binding proteins. We validated that QKI RBFOX1 were necessary sufficient to induce an intermediate mesenchymal cell state increased tumorigenicity. Using RNA-seq eCLIP analysis, coordinately regulated function...
Mitogen-activated protein (MAP) kinase phosphatase 1 (MKP-1) has been shown to play a critical role in mediating the feedback control of MAP cascades variety cellular processes, including proliferation and stress responsiveness. Although MKP-1 expression is induced by broad array extracellular stimuli, mechanisms its induction remain poorly understood. Here we show that mRNA was potently arsenite ultraviolet light modestly increased heat shock hydrogen peroxide. Interestingly, also...
The cucumber (Cucumis sativus L.) exhibits extensive variations in fruit size and shape. Fruit length is an important agronomic domesticated trait controlled by quantitative loci (QTLs). Nonetheless, the underlying molecular genetic mechanisms that determine remain unclear. QTL-seq efficient strategy for QTL identification takes advantage of bulked-segregant analysis (BSA) next-generation sequencing (NGS). In present study, we conducted mapping length. identified 8 QTLs immature mature A...
Isoacteoside (is a phenylethanoid isolated from Monochasma savatieri Franch. ex Maxim., which is an anti-inflammatory herb widely used in traditional Chinese medicine. However, the exact mechanism of activity isoacteoside not completely understood. In this study, its was elucidated mouse macrophages.The expression NF-κB pathway, MAPK iNOS, TNF-α, IL-6 and IL-1β evaluated using Western blotting, quantitative real-time PCR or ELISA. TLR4 dimerization determined by transfecting HEK293T cells...
N6-methyladenosine (m6A), the most prevalent posttranscriptional RNA modification, involved in various diseases and cellular processes.However, underlying mechanisms of m6A regulation skin aging are still not fully understood.In this study, proteomics analysis revealed a significant correlation between Wilms' tumor 1-associating protein (WTAP) expression senescence.Next, upregulated WTAP was detected tissues senescent human dermal fibroblasts (HDFs).Functionally, overexpressed induced...
Metallothioneins are a group of low molecular mass and cysteine-rich metal-binding proteins, ubiquitously found in most living organisms. They play an important role maintaining intracellular metal homeostasis, eliminating toxification protecting against oxidative damages. Analysis complete rice genome sequences revealed eleven genes encoding putative metallothionein (OsMT), indicating that OsMTs constitute small gene family rice. Expression profiling each member the OsMT differs not only...
Arsenic trioxide (As<sub>2</sub>O<sub>3</sub>) is highly effective for the treatment of acute promyelocytic leukemia, even in patients who are unresponsive to all-<i>trans</i>-retinoic acid therapy. As<sub>2</sub>O<sub>3</sub> believed function primarily by promoting apoptosis, but underlying molecular mechanisms remain largely unknown. In this report, using cDNA arrays, we have examined changes gene expression profiles triggered clinically achievable doses leukemia NB4 cells....
Although epidemiological studies have long established that inorganic arsenic is a potent human carcinogen, the underlying mechanisms are still poorly understood. Recent suggest may act as tumor promoter by perturbing key signaling transduction pathways. We shown previously arsenite can potently activate mitogen-activated protein kinase cascades and induce expression of proliferation-associated genes, including proto-oncogenes c-jun c-fos. In order to elucidate further molecular its...
Summary Chromosome painting is a powerful technique for chromosome and genome studies. We developed flexible based on multiplex PCR of synthetic oligonucleotide (oligo) library in cucumber ( Cucumis sativus L., 2 n = 14). Each oligo the was associated with universal as well nested specific primers amplification, which allow generation different probes from same library. were also able to generate double‐stranded labelled oligos, produced much stronger signals than single‐stranded by...
Polyamine depletion and cytokine transforming growth factor-β (TGF-β) inhibit cell proliferation. The current study tests the hypothesis that polyamine results in inhibition by altering expression of TGF-β gene intestinal epithelial cells. Studies were conducted IEC-6 line derived from rat small crypt Cells grown DMEM presence or absence α-difluoromethylornithine (DFMO), a specific inhibitor biosynthesis, for 6 12 days. Administration DFMO not only depleted intracellular polyamines but also...
The present studies describe a new function for calmodulin-dependent protein kinase II (CaM-KII) in signal transduction leading to apoptosis. Both tumor necrosis factor alpha (TNF) and UV light rapidly stimulated Ca2+-independent activity of CaM-KII the monocytic leukemia, U937. Two mechanistically different inhibitors blocked activation prevented DNA fragmentation death. Activation during apoptosis inhibition by two were reproduced several other lines including KGla, HL-60, YAC-1. However,...
Parthenocarpy is an important trait for yield and quality in many plants. But due to its complex interactions with genetic physiological factors, it has not been adequately understood applied breeding production. Finding novel effective quantitative loci (QTLs) a critical step towards understanding mechanism. Cucumber (Cucumis sativus L.) typical parthenocarpic plant but the QTLs controlling parthenocarpy cucumber were mapped on chromosomes, linked markers neither user-friendly nor confirmed...
Leaf color mutants in higher plants are ideal materials for investigating the structure and function of photosynthetic system. In this study, we identified a cucumber vyl (virescent-yellow leaf) mutant library, which exhibited reduced pigment contents delayed chloroplast development process. F2 BC1 populations were constructed from cross between inbred line 'Hazerd' to identify that trait is controlled by simply recessive gene designated as CsVYL. The CsVYL was mapped 3.8 cM interval on...
Differentiation and copy number of repetitive sequences affect directly chromosome structure which contributes to reproductive isolation speciation. Comparative cytogenetic mapping has been verified an efficient tool elucidate the differentiation distribution in genome. In present study, distinct chromosomal structures five Cucumis species were revealed through genomic situ hybridization (GISH) technique comparative major satellite repeats. Chromosome investigated using GISH specific...
Summary Chromosome painting based on fluorescence in situ hybridization ( FISH ) has played an important role chromosome identification and research into rearrangements, diagnosis of abnormalities evolution human animal species. However, it not been applied widely plants due to the large amounts dispersed repetitive sequences chromosomes. In present work, a method for single‐copy gene pools C ucumis sativus was successfully developed. Gene probes with sizes above 2 kb were detected...
Axillary lymph node metastasis (ALNM) is commonly the earliest detectable clinical manifestation of breast cancer when distant emerges. This study aimed to explore influencing factors ALNM and develop models that can predict its occurrence preoperatively.Cases sonographically visible stage T1-2N0M0 cancers treated with axillary surgery at West China Hospital were retrospectively reviewed. Univariate multivariate logistic regression analyses performed evaluate associations between variables....
Sirtuin 5 (SIRT5) is a NAD+ -dependent class III protein deacetylase, and its role in prostate cancer has not yet been reported. Therefore, to explore the diagnosis treatment of cancer, we investigated effect SIRT5 on cancer. was assessed by immunohistochemistry 57 normal cancerous tissues. We found that tissue expression levels patients with Gleason scores ≥7 were significantly different from those <7 (P < .05, R > 0). Further, mass spectrometry pathway screening experiments showed...
The importance of allopolyploidy in plant evolution has been widely recognized. genetic changes triggered by allopolyploidy, however, are not yet fully understood due to inconsistent phenomena reported across diverse species. construction synthetic polyploids offers a controlled approach systematically reveal genomic that occur during the process polyploidy. This study reports first sequenced allopolyploid constructed from cross between Cucumis sativus and C. hystrix, with high-quality...
Heat stress (HS) seriously restricts the growth and development of plants. When plants are exposed to extreme high temperature, heat response (HSR) is activated enable survive. Sessile have evolved multiple strategies sense cope with HS. Previous studies established that PHYTOCHROME INTERACTING FACTOR 4 (PIF4) acts as a key component in thermomorphogenesis; however, whether PIF4 regulates plant thermotolerance molecular mechanism linking this light transcriptional factor HSR remain unclear....
Abstract Background and objectives The study investigated the pathogenesis of Yao syndrome (YAOS), a rare systemic autoinflammatory disease associated with nucleotide-binding oligomerization domain containing 2 ( NOD2 ) gene variants. Methods RNA sequencing analyses were used to detect transcriptomic profile changes. Immunoblot immunohistochemistry examine NOD2-mediated inflammatory signaling pathways ELISA was cytokines. Results Transcriptome analysis YAOS revealed NOD-like receptor pathway...
Abstract Direct exposure of human hepatoma cell line SMMC‐7721 to hydrogen peroxide (H 2 O ) can induce apoptosis. Apoptosis induced by H was inhibited cycloheximide, actinomycin D, 3‐aminobenzamide, EGTA or Zn 2+ . increase the level intracellular Ca , downregulate GSH levels, slightly lipid peroxidation, and lead change in ratio reduced ion components oxidized cells. Analysis flow cytometry indicates that decreases Bcl‐2. The data indicate ‐induced apoptosis requires new mRNA protein...
LncRNA-FGD5-AS1, as an oncogene, participates in the development and progress of various cancers. However, exact role molecular mechanisms by which FGD5-AS1 regulates radiosensitivity breast cancer (BC) remains largely unknown.We used X-Ray weekly-dose-increase method to establish radiation-resistance cell lines. Bioinformatics tools analyze expression tissue evaluated relationship between clinic-pathological features. CCK-8 colony formation were proliferation. Western blotting qPCR applied...