Abstract Background MicroRNA 942-5p (miR-942-5p) has been reported to promote migration and invasion in non-small cell lung cancer (NSCLC), but the underlying mechanism is not completely understood. The interplay between long non-coding RNAs (lncRNAs) miRNAs plays a crucial role tumor progression. Methods In present study, we performed bioinformatic biochemical analyses identify miR-942-5p-interacting lncRNAs. function clinical significance of candidate lncRNA(s) NSCLC were determined....

Radiation resistance poses a major clinical challenge in breast cancer (BC) treatment, but little is known about how long noncoding RNA (lncRNA) may regulate this phenomenon. Here, we reported that DUXAP8 was highly expressed radioresistant BC tissues, and high expression of associated with poor prognosis. We found the overexpression promoted radioresistance, while knockdown increased radiosensitivity. Further studies revealed enhanced radioresistance cells by activating PI3K/AKT/mTOR...

Glypican-3 (GPC3) is a membrane-associated heparan sulfate proteoglycan involved in regulation of cell proliferation, survival, migration and differentiation process. MicroRNAs (miRNAs) are single-stranded, non-coding functional RNAs that important many biological processes. GPC3 miRNAs have been found to play essential roles the development progression hepatocellular carcinoma (HCC). However, little information about relationship between available nowadays. Therefore, this study aims...

Glypican-3 (GPC3), a membrane-associated heparan sulfate proteoglycan, is frequently upregulated in hepatocellular carcinoma (HCC). Yes-associated protein (YAP) also found over-expressed HCC and has been identified as key effector molecule Hippo pathway, which could control the organ size animals through regulation of cell proliferation apoptosis plays an important role development malignant tumors. Studies have reported that GPC3 YAP might collaborate to regulate HCC. To elucidate its...

LncRNA-FGD5-AS1, as an oncogene, participates in the development and progress of various cancers. However, exact role molecular mechanisms by which FGD5-AS1 regulates radiosensitivity breast cancer (BC) remains largely unknown.We used X-Ray weekly-dose-increase method to establish radiation-resistance cell lines. Bioinformatics tools analyze expression tissue evaluated relationship between clinic-pathological features. CCK-8 colony formation were proliferation. Western blotting qPCR applied...

Colorectal cancer is one of the most common malignancies. Previous studies have reported that cortactin (CTTN) often overexpressed in tumors and associated with metastasis poor prognosis patients. The abnormal expression microRNAs (miRNAs or miRs) closely related to development progression various types cancer, including colorectal cancer. However, little known about miRNAs targeting cortactin. In present study, prediction using biological software revealed has binding sites for miR-542-3p....

MicroRNAs and Yes‐associated protein (YAP) play an important role in the occurrence development of hepatic carcinomas. However, interaction between microRNAs YAP was seldom elucidated. In this study, we showed that miR‐132 could target gene by using dual‐luciferase reporter system. Further quantitative polymerase chain reaction analysis western blotting significantly reduce expression at mRNA levels. Results annexin V‐fluorescein isothiocyanate, 5‐ethynyl‐2′‐deoxyuridine staining transwell...

Glypican‑3 (GPC3) is a membrane heparan sulfate proteoglycan involved in cell proliferation, differentiation, adhesion, migration and the development of majority mesodermal tissues organs. GPC3 has been found to be important for occurrence hepatocellular carcinoma (HCC). Therefore, it may suitable use as novel molecular marker diagnosis primary liver cancer. In present study, role HCC was determined. recombinant vector transfected into two lines, Huh7 SK‑HEP‑1, upregulate expression examine...

Colon cancer is one of the most common malignancies worldwide. Because side effects and defects in tolerance chemotherapy, it necessary to discover new drugs for colon treatment. Columbamine has been identified as an effective anti-osteosarcoma compound with only minor effects. In this study, we analyzed anticancer effect columbamine on cancer.Human cell lines were treatment columbamine. MTT assay, colony formation apoptosis detection tumorigenicity assay performed detect protective...

To investigate the effects of co-transfection miR-520c-3p and miR-132 on proliferation apoptosis hepatocellular carcinoma Huh7.Hepatocellular Huh7 was cultured in vitro lipidosome used to transfect miR-132, respectively or together. The transfection were detected by CCK8 Annexin V staining flow cytometry, expression level targeted gene over-expressed determined Western blot realtime PCR.Compared with control group, ability single transfected co-transfected decreased significantly, ratio...

Abstract Background Long non-coding RNA is considered to be essential modulate the development and progression of human malignant cancers. And long can act as crucial modulators by sponging corresponding microRNA in tumorigenesis. We aimed elucidate function ACTA2-AS1 its molecular mechanism colon adenocarcinoma. Materials methods The expression ACTA2-AS1, miR-4428 BCL2L11 adenocarcinoma tissues were detected via qRT-PCR. SW480 HT29 cells transfected with shRNA OE miRNA mimics miR-4428,...

To study the change of peripheral blood immune pattern and its correlation with prognosis in patients liver cancer after treated by sorafenib.Patients advanced admitted our hospital were enrolled sorafenib. After two months treatment, their was collected. The cell subset cytokines level determined flow cytometry luminex technology. According to reaction expressed towards sorafenib, divided into response group no group. changes groups compared.Before treatment there significant difference...

Acute lung injury (ALI) is a significant health condition with notable rates of morbidity and mortality globally. Long non-coding ribose nucleic acids (lncRNAs) play vital roles in mitigating various inflammation-related diseases, including ALI. The study aimed to investigate the functional role molecular mechanisms lncRNA SNHG1 on ALI lipopolysaccharide (LPS)-treated A549 cells LPS-induced mice. expression was initially examined LPS-treated cells. We further demonstrated critical function...

Diabetic nephropathy (DN) is a common and severe complication of diabetes, impacting millions people worldwide. High concentrations serum glucose-associated injury renal glomerular endothelial cells (rGECs) are involved in the DN pathogenesis. We found that exposure to high glucose increased expression angiotensin II type 1 receptor (AT1R) human rGECs (hrGECs). To block AT1R level, we used newly developed antagonist Telmisartan. This study investigated whether Telmisartan possessed...

Curative effect and adverse reactions of oxaliplatin combined with endostar in the interventional treatment primary hepatic carcinoma (PHC) were investigated. A total 101 PHC patients from October 2012 to December 2014 The First Affiliated Hospital Xi'an Jiaotong University retrospectively collected. Fifty therapy group treated endostar, while remaining 51 alone. lasted for a 4 cycles (20 days as 1 cycle). ratios cluster differentiation 3 (CD3)+, CD4+ CD8+ detected via enzyme-linked...

To build GPC3 gene short hairpin interference RNA (shRNA) slow virus vector, observe expression of Huh-7 in human liver cell line proliferation apoptosis and the effect influencing on cancer growth, provide theoretical basis for therapy cancer.Hepatocellular carcinoma was transfected by a technique. variety lines detected fluorescence quantitative PCR. Targeted sequences small interfering (siRNA) PGC-shRNA-GPC3 were restructured. Stable siRNA screened established with help liposomes...

To study the effect of lipoteichoic acid (LTA) and 5-FU on expression caspase-3, EGFR, TGF-α proteins tumor tissue H22 cancer bearing mice its anti-tumor mechanism. A total 40 SPF grade Kunming were selected to establish liver model, then divided into 4 groups at random with ten in each group. Group was given saline lavage treatment, B treated by intraperitoneal injection, C LTA lump body injection; D injection injection. Two weeks after group executed stripping weighted, growth inhibition...

Hepatocellular carcinoma (HCC) is one of the most common types malignant tumor, and second highest cause cancer‑associated mortality, behind lung carcinoma. It urgent to identify novel genes that can be used confirm diagnosis prognosis patients with HCC. The present study aimed investigate expression pattern phosphatidylinositol glycan anchor biosynthesis class C (PIGC) in HCC assess its clinical prognostic significance. Bioinformatics analyses were PIGC mRNA levels adjacent non‑cancerous...