We investigated the ability of targeted immunomicelles to detect and assess macrophages in atherosclerotic plaque using MRI vivo . There is a large clinical need for noninvasive tool atherosclerosis from molecular cellular standpoint. Macrophages play central role are associated with plaques vulnerable rupture. Therefore, macrophage scavenger receptor (MSR) was chosen as target MRI. MSR-targeted immunomicelles, micelles, gadolinium–diethyltriaminepentaacetic acid (DTPA) were tested ApoE−/−...

A new contrast agent for MRI based on recombinant HDL-like nanoparticles has been prepared. It shows a great potential as atherosclerotic plaques in relative short time (24 h post-injection) it is selective the and an endogenous molecule. also can distinguish between different types of enhancement obtained different, depending plaque composition.

The need for more specific and selective contrast agents magnetic resonance imaging motivated us to prepare a new nanoparticle agent based on high-density lipoproteins (HDL). This second generation can be prepared in three different ways. HDL nanoparticles (rHDL) were fully characterized by FPLC gel electrophoresis. flexibility of the platform also allows incorporate optical probes into rHDL localization ex vivo confocal fluorescence microscopy. contrast-agent-containing injected mice that...

Virtually all mesenchymal stem cell (MSC) studies assume that therapeutic effects accrue from local myocardial of engrafted MSCs. Because few intravenously administered MSCs engraft in the myocardium, have mainly utilized direct delivery. We adopted a different paradigm.To test whether reduce left ventricular (LV) dysfunction both post-acute infarction and ischemic cardiomyopathy these are caused, at least partly, by systemic anti-inflammatory activities.Mice underwent 45 minutes anterior...

Abstract The ability to specifically image macrophages may enable improved detection and characterization of atherosclerosis. In this study we evaluated the in vitro uptake gadolinium (Gd)‐containing immunomicelles (micelles linked macrophage‐specific antibody), micelles, standard contrast agents by murine macrophages, sought determine whether micelles improve ex vivo imaging apolipoprotein E knockout (ApoE KO) Murine RAW 264.7 were incubated with Gd‐DTPA, immunomicelles. Cell pellets...

The synthesis and photophysical characterisation are reported of a series cationic, neutral anionic europium terbium complexes based on structurally related, nonadentate ligands the cyclen macrocycle. Each complex incorporates tetraazatriphenylene moiety overall absolute emission quantum yields in range 15–40% aerated aqueous media. Dynamic quenching lanthanide excited state occurs with electron-rich donors, e.g. iodide, ascorbate urate, mechanistic interpretation is put forward involving an...

Abstract Pegylated, fluorescent, and paramagnetic micelles were developed. The conjugated with macrophage scavenger receptor (MSR)‐specific antibodies. abdominal aortas of atherosclerotic apoE‐KO mice imaged T 1 ‐weighted high‐resolution MRI before 24 h after intravenous administration the contrast agent (CA). Pronounced signal enhancement (SE) (up to 200%) was observed for apolipoprotein E knockout (apoE‐KO) that injected MSR‐targeted micelles, while aortic vessel wall nontargeted showed...

DNA interaction with scorpiand azamacrocycles has been achieved through modulation of their binding affinities. Studies performed different experimental techniques provided evidence that pH or metal-driven molecular reorganizations these ligands regulate ability to interact calf thymus (ctDNA) an intercalative mode. Interestingly enough, serve increase decrease the biological activities compounds significantly.

The interaction of q = 0 Δ- and Λ-Tb Eu complexes with poly(dAdT), poly(dGdC) calf-thymus DNA has been examined by absorption, emission chiroptical spectroscopy is sensitive to complex helicity, base-pair type the nature lanthanide excited state.

Nonacoordinate delta- and lambda-Eu Tb complexes have been tested as imaging reactive probes in mouse fibroblast (NIH 3T3) cells. The uptake of these by the cells was assessed fluorescence microscopy. Complex-induced DNA damage studied gel electrophoresis shown to be a function complex chirality.

Contrast-enhanced MRI of atherosclerosis can provide valuable additional information on a patient's disease state. As result the interactions HDL with atherosclerotic plaque and flexibility its reconstitution, it is versatile candidate for delivery contrast-generating materials to this pathogenic lesion. We herein discuss reports modified gadolinium act as an contrast agent atherosclerosis. Furthermore, has been fluorophores nanocrystals, allowing fluorescent imaging techniques computed...

In cationic nine-coordinate chiral terbium and europium complexes incorporating exciton-coupled naphthyl groups a tetraazatriphenylene sensitising chromophore, efficient intramolecular energy transfer occurs leading to population of the triplet state. With complex, absolute quantum yield singlet oxygen formation is 51% (λexc 355 nm), for Eu complex intensity metal-based emission increases by up 350% on binding poly(dGdC) or calf-thymus DNA, was greater Δ-isomer.

The synthesis and steady-state fluorescence studies on the interaction with AMPH, METH, MDMA, DA of two diazatetraester pyrazole crowns containing appended N-(9H-fluoren-9-yl) N-(naphth-2-ylmethyl) functions, in a water/ethanol 70:30 mixture at physiological pH, are described.

We present a set of donor radii for the rare-earth cations obtained from analysis structural data available in Cambridge Structural Database (CSD). Theoretical calculations using density functional theory (DFT) and wave function approaches (NEVPT2) demonstrate that Ln-donor distances can be broken down into contributions cation atom, with minimum electron (ρ) defines position (3,–1) critical points corresponding well Shannon's crystal (CR). Subsequent linear fits experimental bond all rare...

The synthesis and interaction of N,N',N'',N'''-tetramethyl-2,6,9,13-tetraaza[14]paracyclophanes (B323Me(4)) with nucleotides inorganic phosphates is described. An easy methodology used to define thermodynamic selectivity. B323Me4 ATP has been monitored by (1)H, (31)P NMR molecular mechanics, ruling out the possibility participation pi-stacking interactions. Results show that N-methylation accompanied a strong increase in resulting macrocycle ATP.

The synthesis of a polyazamacrocycle constituted by two diethylenetriamine bridges functionalized at their central nitrogen with naphth-2-ylmethyl units and interconnected through 2,6-dimethylpyridine spacers (L1) is reported. protonation behaviour the new macrocycle in water water-ethanol 70/30 v/v mixed solvent has been examined means pH-metric, UV-Vis steady-state fluorescence techniques. emission slightly quenched following deprotonation tertiary amines more deeply upon secondary amino...

A synthetic procedure for the preparation of functional structured inorganic–organic hybrid materials consisting boehmite-silica core–shell nanoparticles and anthracene-containing amines covalently attached to surface is reported. The system functionalised with monoamine chain shows a very high sensing performance Hg2+ detection in pure water reaching limit 0.2 ppb. Two additional advantages these systems are their stability over wide pH window feasibility be recovered by simple procedure.

The synthesis of boehmite nanoparticles modified with lanthanides (Eu, Tb and Gd) is described. Their synthesis, characterization in vitro assays HeLa cells were performed. nuclear magnetic relaxation dispersion (NMRD) profiles the two chelating moieties studied. Imaging data from laser scanning confocal fluorescence microscopy flow cytometry revealed that nanoscaffolds taken up by cells, distributed throughout cytoplasm showed no toxicity. This platform could represent an alternative to...