A5081890263- Microbial Natural Products and Biosynthesis
- Marine Sponges and Natural Products
- Fungal Biology and Applications
- Peptidase Inhibition and Analysis
- Plant biochemistry and biosynthesis
- Renin-Angiotensin System Studies
- Chemokine receptors and signaling
- Microbial Metabolites in Food Biotechnology
- Atherosclerosis and Cardiovascular Diseases
- Genomics and Phylogenetic Studies
- Medicinal plant effects and applications
- Neonatal Respiratory Health Research
- Immune Response and Inflammation
- Ultrasound and Hyperthermia Applications
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Acute Kidney Injury Research
- Plant-Microbe Interactions and Immunity
- Pericarditis and Cardiac Tamponade
- Biomarkers in Disease Mechanisms
- Smart Agriculture and AI
- Facial Trauma and Fracture Management
- Signaling Pathways in Disease
- Chromatography in Natural Products
- Phytochemical Studies and Bioactivities
- Neuropeptides and Animal Physiology
Anhui Medical University
2022-2024
Henan University
2021-2024
Sun Yat-sen University
2014-2024
Nagasaki University
2024
Guangxi Medical University
2024
Guiyang Medical University
2022
Wuhan Polytechnic University
2021
Cleveland Clinic
2021
And Technology Research (United Kingdom)
2021
Guangdong Provincial People's Hospital
2018
Three new 12- or 13-membered-ring macrocyclic alkaloids, named ascomylactams A–C (1–3), along with the analogues phomapyrrolidone C (4) and A (5) were isolated from mangrove endophytic fungus Didymella sp. CYSK-4. Their structures elucidated by analysis of extensive spectroscopic data mass spectrometric data. The absolute configurations 1 2 determined single-crystal X-ray diffraction experiments, which represents first crystal described for a (6/5/6/5) tetracyclic skeleton fused moiety....
Molecular networking guidance lead to the isolation of a new cyclopenta[ b ]fluorene-type alkaloid didymorenloid A (1), containing an unprecedented 6/5/6/5/13(6)/5/5/5-fused ring system, from mangrove endophytic fungus Didymella sp. CYSK-4.
Pleosmaranes A–R (1–18), 18 new isopimarane-type diterpenoids, together with four known analogs (19–22), were isolated from the mangrove endophytic fungus Pleosporales sp. HNQQJ-1. Their structures and absolute configurations established by analysis of their spectroscopic data electronic circular dichroism (ECD) calculations. Compounds 1–9 possess an unusual aromatic B ring a 20-nor-isopimarane skeleton. 15–17 contain unique 2-oxabicyclo[2.2.2]octane moiety. Compound features unexpected...
Hyperuricemia (HUA) is a metabolic disease, closely related to oxidative stress and inflammatory responses, caused by reduced excretion or increased production of uric acid. However, the existing therapeutic drugs have many side effects. It imperative find drug an alternative medicine effectively control HUA. was reported that Gardenia jasminoides Poria cocos could reduce level acid in hyperuricemic rats through inhibition xanthine oxidase (XOD) activity. But there were few studies on its...
Synthetic biology is an effective way to activate silent biosynthetic gene clusters. Five new indole diterpenoids (1, 2, 5, 9, and 10), together with 10 known derivatives (3, 4, 6–8, 11–15) were activated from Aspergillus oryzae transformants by exogenous P450 Ast B obtained under the guidance of molecular networking. Their planar structures determined NMR HR-ESI-MS. The absolute configuration compound 1 was single crystal X-ray diffraction, those compounds 5 , confirmed comparing observed...
Background: Apoptosis has been demonstrated to be an important upstream event in the pathogenesis of chronic obstructive pulmonary disease (COPD). Cyclooxygenase-2 (COX-2) seems biologically relevant COPD. However, role COX-2 apoptosis vascular endothelial cells induced by cigarette smoke extract (CSE) remains elucidated. Our recent study found that prostacyclin, one COX products microvascular endothelium, inhibited emphysematous lungs rats CSE. In order clarify CSE, we performed present...
The synthetic biology approach enables efficient and directional mining of target compounds during drug discovery. Ten new asperterpenoids (6–15) five known analogues (1–5), possessing a rare 5/7/3/6/5 skeleton, were obtained from two Aspergillus oryzae transformants with heterologous expression terpene cyclase gene AstC one or P450 genes AstB/A under the guidance molecular networking. Their planar structures determined by 1D 2D NMR HR-ESI-MS. absolute configurations 6 9–13 single crystal...
Ascomylactam C (AsC) is a new 13-membered-ring macrocyclic alkaloid, which was first isolated and identified in 2019 from the secondary metabolites of mangrove endophytic fungus Didymella sp. CYSK-4 South China Sea. AsC has been found to have broad-spectrum cytotoxic activity. However, antitumor effects vivo mechanisms remain unclear. The aim this study describe on lung cancer melanoma cells explore molecular mechanism AsC. In vitro, we used plate colony formation experiments demonstrated...
Smoking causes lung endothelial cell apoptosis and emphysema. Derived from bone marrow, circulating progenitor cells (EPCs) maintain vascular integrity by replacing repairing damaged cells. influences the number of EPCs. Recruitment EPCs marrow to peripheral blood depends on interaction c-Kit/soluble c-Kit ligand (sKitL). We hypothesized that smoking might influence c-Kit(+) EPCs/sKitL in development smoking-related In this study, we used a cigarette smoke extract (CSE)-induced emphysema...
Six previously undescribed cytosporone derivatives (phomotones A-E (1-5) and phomotone F (13)), two new spiro-alkanol phombistenes A-B (14-15), seven known analogs (6-12) were isolated from the mangrove endophytic fungus Phomopsis sp. QYM-13. The structures of these compounds elucidated using spectroscopic data analysis, electronic circular dichroism (ECD), 13C NMR calculations. Compound 14 features an unprecedented 1,6-dioxaspiro[4.5]decane ring system. All isolates evaluated for their...
In the poultry slaughtering, accurate viscera positioning is essential to reduce damage of internal organs. The introduction machine vision technique can help locate and provide new direction for evisceration. After a midline abdominal incision poultry, organs are taken out from that placed on conveyor line by parallel robot. Based vision, recognition accuracy opened directly affects level visceral residue caused gripping manipulator. However, often influenced different noise in cavity, such...
Calcitonin gene-related peptide (CGRP) has a potent protective action on the cardiovascular system; however, little is known about role of CGRP in angiotensin II- (Ang II-) induced inflammation vascular smooth muscle cells (VSMCs). This study aimed at determining anti-inflammatory effect Ang II-treated VSMCs and whether disintegrin metalloproteinase 17 (ADAM17) modulates this action. Small interference RNA (siRNA) inhibitors CGRP, epidermal growth factor receptor (EGFR), extracellular...
A new kavalactone, 4'-hydroxyl dihydro-5, 6-dehydrokavain (1) was isolated from the petroleum ether partition of leaves Alpinia zerumbet (Pers.) Burtt. et Smith, together with four known kavalactone dimers, rel-1,trans-3-bis-(4-methoxy-2-oxopyran-6-yl)-cis-2,trans-4-diphenyl cyclobutene (2), aniba dimer (3), C (4), 6,6'-(3,4-diphenylcyclobutane-1,2-diyl)bis(4-methoxy-2H-pyran-2-one (5). The structure compound 1 characterized by its MS, 1D-NMR, and 2D-NMR data, structures compounds were...
Obstructive uropathy is a common kidney disease caused by elevated hydrostatic pressure (HP), but relevant molecular and cellular mechanisms have not yet been well understood. In this study, we ex vivo investigated the effects of HP on human renal epithelial cells (HREpCs). Primary HREpCs were subjected to 100 cmH2O for 8 or 48 h. Then, cultured without stimulation another 24 h 72 Cell morphology showed almost no change after 8h treatment, exhibited reversible elongation 48h treatment....
Abstract: A systemic and local inflammatory immune imbalance is thought to be the cause of traumatic tracheal stenosis (TS). However, with CD4+ T lymphocytes being predominant cells in TS, mechanism action recruitment has not been described. In our research, using flow cytometry, ELISA, immunofluorescence, Transwell chamber assays, expression, distribution, potential chemotactic function TS patients were examined before after treatment. The results showed that untreated group had...