Molecular networking guidance lead to the isolation of a new cyclopenta[ b ]fluorene-type alkaloid didymorenloid A (1), containing an unprecedented 6/5/6/5/13(6)/5/5/5-fused ring system, from mangrove endophytic fungus Didymella sp. CYSK-4.

We have identified the biosynthetic gene cluster (hvm) for sterol O-acyltransferase inhibitor helvamide (1) from genome of Aspergillus rugulosus MST-FP2007. Heterologous expression hvm in A. nidulans produced a previously unreported analog B (5). An α-ketoglutarate-dependent oxygenase Hvm1 was shown to catalyze intramolecular cyclization 1 yield 5. The branch related hancockiamides and helvamides found be controlled by substrate selectivity monomodular nonribosomal peptide synthetases.

Two new octaketides, cytosporones W (1) and X (2), along with eight known cytosporone derivatives [(±)-3-9], were isolated from mangrove endophytic fungus Diaporthe sp. ZJHJYZ-1. Compounds 1 2 a pair of epimers, whose configuration C-1 could mutually convert, causing racemization the lactone ring. The planar structures compounds elucidated through detailed 1D, 2D NMR, HR-ESI-MS analysis. ECD spectra comparison modified Mosher ester method applied to determine absolute 2. In bioassays, (±)-3...

A new depsidone derivative (1), aspergillusidone G, was isolated from a marine fungus Aspergillus unguis, together with eight known depsidones (2‒9) and cyclic peptide (10): agonodepside (2), nornidulin (3), nidulin (4), F (5), unguinol (6), C (7), 2-chlorounguinol (8), (9), unguisin (10). Compounds 1‒4 7‒9 were obtained the plasma induced mutant of this fungus, while 5, 6, 10 original strain under chemical induction. Their structures identified using spectroscopic analysis, as well by...

One new diterpenoid, diaporpenoid A (1), two sesquiterpenoids, diaporpenoids B–C (2,3) and three α-pyrone derivatives, diaporpyrones A–C (4–6) were isolated from an MeOH extract obtained cultures of the mangrove endophytic fungus Diaporthe sp. QYM12. Their structures elucidated by extensive analysis spectroscopic data. The absolute configurations determined electronic circular dichroism (ECD) calculations a comparison specific rotation. Compound 1 had unusual 5/10/5-fused tricyclic ring...

Two new 3-decalinoyltetramic acid derivatives with peroxide bridge fusarisetins E (1) and F (2), one chromone fusarimone A (5), two benzofurans fusarifurans (9) B (10), three isocoumarins fusarimarins A-C (11-13), as well five known analogues 3, 4, 6-8 14 were isolated from mangrove endophytic fungus Fusarium sp. 2ST2. Their structures absolute configurations established by spectroscopic analysis, density functional theory-gauge invariant atomic orbital NMR calculation DP4+ statistical...

Synthetic biology is an effective way to activate silent biosynthetic gene clusters. Five new indole diterpenoids (1, 2, 5, 9, and 10), together with 10 known derivatives (3, 4, 6–8, 11–15) were activated from Aspergillus oryzae transformants by exogenous P450 Ast B obtained under the guidance of molecular networking. Their planar structures determined NMR HR-ESI-MS. The absolute configuration compound 1 was single crystal X-ray diffraction, those compounds 5 , confirmed comparing observed...

Five new polyketides, including two chromones (1-2), phenyl derivatives (4-5), and a tandyukusin derivative (6), along with five known polyketides (3 7-10) were isolated from mangrove endophytic fungus Trichoderma lentiforme ML-P8-2. The planar structures of compounds elucidated via detailed 1D, 2D NMR, HR-ESI-MS analysis. ECD spectra, optical rotation values calculation, alkali hydrolysis applied in the determination absolute configuration compounds. In bioassays, 6 9 exhibited promising...

Mangrove endophytic fungi represent significant and sustainable sources of novel metabolites with unique structures excellent biological activities, attracting extensive chemical investigations. In this research, two heterodimeric tetrahydroxanthones, aflaxanthones A (1) B (2), dimerized via an unprecedented 7,7′-linkage, a sp3-sp3 dimeric manner, were isolated from the mangrove fungus Aspergillus flavus QQYZ. Their elucidated through high resolution electrospray ionization mass spectroscopy...

Four new compounds including three andrastin-type meroterpenoids hemiacetalmeroterpenoids A-C (1-3), and a drimane sesquiterpenoid astellolide Q (15), together with eleven known (4-14) were isolated from the cultures of marine-derived fungus Penicillium sp. N-5, while compound 14 was first natural source. The structures determined by analysis detailed spectroscopic data, absolute configurations further decided comparison experimental calculated ECD spectra. Hemiacetalmeroterpenoid A (1)...

The synthetic biology approach enables efficient and directional mining of target compounds during drug discovery. Ten new asperterpenoids (6–15) five known analogues (1–5), possessing a rare 5/7/3/6/5 skeleton, were obtained from two Aspergillus oryzae transformants with heterologous expression terpene cyclase gene AstC one or P450 genes AstB/A under the guidance molecular networking. Their planar structures determined by 1D 2D NMR HR-ESI-MS. absolute configurations 6 9–13 single crystal...

Main observation and conclusion Co‐culturing the mangrove endophytic fungus Penicillium sclerotiorum THSH‐4 with ZJHJJ‐18 in PDB medium resulted production of nine new azaphilones derivatives, peniazaphilones A—I (1—9), six known derivatives (10—15). Their structures were elucidated by analysis detailed spectroscopic data, single‐crystal X‐ray diffraction, ECD spectra, 13 C NMR calculation DP4+ analysis. It is noteworthy that compound 1 an isoquinoline quinone moiety, was isolated from...

Abstract Cholinergic disorder, oxidative stress, and neuroinflammation play important roles in the pathology of Alzheimer's disease. To explore healthy potential edible seaweed Hizikia fusiforme on this aspect, a functional oil (HFFO) was extracted from alga investigated its constituents by gas chromatography‐mass spectrometry (GC/MS) study. Its anti‐Alzheimer's related bioactivities including acetylcholinesterase (AChE) inhibition, antioxidation, anti‐neuroinflammation were evaluated,...

Abstract Marine fungi are potentially important resources for bioactive lead compounds discovering new drugs diseases such as Alzheimer’s disease. In this paper, the combined bioassay model of acetylcholinesterase (AChE) inhibition, 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, and Artemia larval lethality was used to evaluate activity toxicity 35 marine fungal strains from seas around China. Their constituents were revealed by thin layer chromatography (TLC) autography,...

Seven new polyketides, including four indenone derivatives, cytoindenones A–C (1, 3–4), 3′-methoxycytoindenone A (2), a benzophenone derivative, cytorhizophin J (6), and pair of tetralone enantiomers, (±)-4,6-dihydroxy-5-methoxy-α-tetralone (7), together with known compound (5) were obtained from the endophytic fungus Cytospora heveae NSHSJ-2 isolated fresh stem mangrove plant Sonneratia caseolaris. Compound 3 represented first natural monomer substituted by two benzene moieties at C-2 C-3....

Abstract Two new benzopyran derivatives, (2 R ,4 S )‐5‐methoxy‐2‐methyl‐3,4‐dihydro‐2 H ‐1‐benzopyran‐4‐ol and ,2′ ,4′ )‐4,4′‐oxybis(5‐methoxy‐2‐methyl‐3,4‐dihydro‐2 ‐1‐benzopyran), a aliphatic compound, (3 E ,5 Z ,8 ,10 )‐8‐hydroxytrideca‐3,5,10,12‐tetraen‐2‐one, together with three known were obtained from mangrove endophytic fungus Penicillium citrinum QJF‐22 collected in Hainan island. Their structures determined by analysis of spectroscopic data the relative configuration was also...