A5072781573- Cell death mechanisms and regulation
- RNA Interference and Gene Delivery
- ATP Synthase and ATPases Research
- IL-33, ST2, and ILC Pathways
- Eosinophilic Esophagitis
- Hepatitis B Virus Studies
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Cancer-related molecular mechanisms research
- Immune Cell Function and Interaction
- Monoclonal and Polyclonal Antibodies Research
- RNA modifications and cancer
- PI3K/AKT/mTOR signaling in cancer
- interferon and immune responses
- HER2/EGFR in Cancer Research
- Antimicrobial Resistance in Staphylococcus
- Animal Virus Infections Studies
- Hepatocellular Carcinoma Treatment and Prognosis
- Bacterial biofilms and quorum sensing
- Histone Deacetylase Inhibitors Research
- SARS-CoV-2 and COVID-19 Research
- Toxin Mechanisms and Immunotoxins
- Mitochondrial Function and Pathology
- Autophagy in Disease and Therapy
- Plant-Microbe Interactions and Immunity
University Medical Center HCMC
2018-2024
University of Toronto
2015-2024
Sunnybrook Research Institute
2019-2024
Sunnybrook Health Science Centre
2019-2024
Massachusetts General Hospital
2024
Harvard University
2024
Sunnybrook Hospital
2019-2023
Muscular Dystrophy Canada
2019
University of California, Irvine
2016
Justus-Liebig-Universität Gießen
2009
Abstract Induction of reactive oxygen species (ROS), an important process for the cytotoxicity various acute myeloid leukemia (AML) therapies including hypomethylating agents (HMAs), concurrently activates NF‐E2‐related factor 2 (Nrf2) antioxidant response pathway which in turn results induction enzymes that neutralize ROS. In this study, we demonstrated Nrf2 inhibition is additional mechanism responsible marked antileukemic activity AML seen with combination HMAs and venetoclax (ABT‐199)....
Pseudomonas syringae is a Gram-negative bacterium that infects multiple plant species by manipulating cellular processes via injection of type three secreted effectors (T3SEs) into host cells. Nucleotide-binding leucine-rich repeat (NLR) resistance (R) proteins recognize specific T3SEs and trigger robust immune response, called effector-triggered immunity (ETI), which limits pathogen proliferation often associated with localized programmed cell death, known as the hypersensitive response...
The Bcl-2 family BH3 protein Bim promotes apoptosis at mitochondria by activating the pore-forming proteins Bax and Bak inhibiting anti-apoptotic Bcl-XL, Mcl-1. binds to these via its domain mitochondrial membrane a carboxyl-terminal sequence (CTS). In cells killed Bim, expression of mutant in which CTS was deleted (BimL-dCTS) triggered that correlated with inhibition being sufficient permeabilize isolated from same cells. Detailed analysis molecular mechanism demonstrated BimL-dCTS...
The proapoptotic BCL-2 homology (BH3)-only endoplasmic reticulum (ER)-resident protein interacting killer (BIK) positively regulates mitochondrial outer membrane permeabilization, the point of no return in apoptosis. It is generally accepted that BIK functions at a distance from mitochondria by binding and sequestering antiapoptotic proteins ER, thereby promoting ER calcium release. Although predominantly localized to we detect fluorescence lifetime imaging microscopy-FRET microscopy, BH3...
Abstract The Bcl-2 family controls apoptosis by direct interactions of pro- and anti-apoptotic proteins. principle mechanism is binding the BH3 domain pro-apoptotic proteins to hydrophobic groove siblings, which therapeutically exploited approved BH3-mimetic anti-cancer drugs. Evidence suggests that also transmembrane (TMD) affects interactions. We developed a highly-specific split luciferase assay, enabling analysis TMD pore-forming effectors BAX, BAK, BOK with in living cells. confirm...
Abstract The ErbB3‐binding protein 1 (Ebp1) has been reported as either an oncogenic regulator or a tumor suppressor in variety of cancers. Here, we show that Ebp1 p48, predominant expression isoform, is highly expressed the majority human colon cells compared with normal adjacent tissues and its required for activities these cells. Depletion primary cancer inhibits cell proliferation, colony forming, invasion vitro well formation vivo enhances sensitivity to irradiation. We further...
Abstract Self-cleaving ribozymes fold into intricate structures, which orient active site groups catalytically competent conformations. Most ribozyme families have distinct catalytic cores stabilized by tertiary interactions between domains peripheral to those cores. We show that large hepatitis delta virus (HDV)-like are activated bring two helical segments, P1 and P2, proximity – a “pinch” results in rate acceleration almost three orders of magnitude. Kinetic analysis with systematically...
Linezolid is one of the last resort antibiotics effectively used in treatment infections caused by multidrug-resistant Gram-positive bacteria. Recent outbreaks resistance have been great concern worldwide, while many countries not experienced it. In this work, we aimed to evaluate existence linezolid and further clarify potential mechanism(s) staphylococcal isolates obtained from hospital Vietnam, a country which had previously detected. Seventy clinical including MRSA (n = 63)...
Anti-apoptotic proteins such as BCL-XL promote cell survival by sequestering pro-apoptotic BCL-2 family members, an activity that frequently contributes to tumorigenesis. Thus, the development of small-molecule inhibitors for anti-apoptotic proteins, termed BH3-mimetics, is revolutionizing how we treat cancer. BH3 mimetics kill cells displacing sequestered initiate tumor-cell death. Recent evidence has demonstrated in live BH3-only PUMA and BIM resist displacement while others like tBID do...
Abstract The Bcl-2 family BH3 protein Bim promotes apoptosis at mitochondria by activating the pore forming proteins Bax and Bak inhibiting anti-apoptotic Bcl-XL, Mcl-1. binds to these via its domain mitochondrial membrane a carboxyl-terminal sequence (CTS). In cells killed Bim, expression of mutant in which CTS was deleted (BimL-dCTS) triggered variable amounts that correlated with inhibition being sufficient permeabilize isolated from same cells. Detailed analysis molecular mechanism...
Abstract Objectives Interleukin (IL)-33 is a novel member of pro-inflammatory cytokine IL-1 family, which plays an important role in the immune response. IL-33 was proved to involve many inflammatory and allergic diseases, thus inhibition this may be promising treatment for these diseases. Arms study were generate mouse soluble receptor fused with human IgG1 Fc domain (msIL33R-Fc) expressed by Pichia pastoris yeast characterize inhibitory activity protein. Methods Clone P. expressing...
SARS-CoV-2 infects the host through interaction be-tween spike protein and ACE2 (Angiotensin convert-ing enzyme 2) receptor on cell surface. There-fore, is considered as a key target in drug devel-opment against COVID-19. In this study, we generated truncated recombinant human (trhACE2) expressed Escherichia coli evaluated its binding activity to of SARS-CoV-2. The sequence (18-119 aa) was cloned into pET28a(+) plasmid transformed E. DH5α strain finally transferred BL21 (DE3) cells for...
Abstract Transcription initiation factor 90 (TIF‐90), an alternatively spliced variant of TIF‐IA, differs by a base pair deletion exon 6. TIF‐90 has been shown to regulate ribosomal RNA (rRNA) synthesis interacting with polymerase I (Pol I) during the DNA (rDNA) transcription in nucleolus. Recently, we showed that TIF‐90‐mediated rRNA can play important role driving tumorigenesis human colon cancer cells. Here show binds GTP at threonine 310, and binding is required for TIF‐90‐enhanced...
Abstract Anti-apoptotic proteins such as BCL-X L promote cell survival by sequestering pro-apoptotic BCL-2 family members, an activity that frequently contributes to tumorigenesis. Thus, the development of small-molecule inhibitors for anti-apoptotic proteins, termed BH3-mimetics, is revolutionizing how we treat cancer. BH3 mimetics kill cells displacing sequestered initiate tumor-cell death. Recent evidence has demonstrated in live BH3-only PUMA and BIM resist displacement while others like...
CD20 is a potential target for immunotherapy treatments of haematological malignancies due to its specific expression and unique biological property. Over the years, many anti-CD20 monoclonal antibodies (mAbs) have been developed, such as Rituximab, Ibritumomab, Tositumomab, etc., applied in B cells related cancer treatments. Recently, new immunological therapy, namely Chimeric antigen receptor (CAR) T-cell which patient’s T are reprogrammed ex vivo with transgene encoding CAR order active...
Hepatocellular carcinoma (HCC) is amongst the most common cancers in world and Vietnam. Most HCC patients are diagnosed late, so effect of treatment limited. Hence, early diagnosis for high-risk populations using medical imaging serum markers very important to control HCC. Recently, α-fetoprotein (AFP) has been popularly used as a marker screening Unfortunately, AFP not trustworthy enough diagnosis, especially Therefore, this study was deployed estimate ability Des-γ carboxy prothrombin...
Positive control (or standard) is an indispensable ingredient in molecular biology assays widely used for the quantification of nucleic acid. The commonly standards are plasmid DNA, cDNA, or naked RNA, which unstable and easily degraded by nucleases surrounding environment; this might affect accuracy quantitative results. In study, authors designed created a positive hepatitis C virus (HCV) based on armored RNA technology. 5’UTR non-encoding sequence HCV was cloned into BH20 plasmid. Armored...