A5101907633- Microbial Metabolic Engineering and Bioproduction
- Algal biology and biofuel production
- Biofuel production and bioconversion
- Microbial metabolism and enzyme function
- Metabolomics and Mass Spectrometry Studies
- Gene Regulatory Network Analysis
- Analytical chemistry methods development
- Photosynthetic Processes and Mechanisms
- Mitochondrial Function and Pathology
- Enzyme Catalysis and Immobilization
- Marine and coastal ecosystems
- Viral Infectious Diseases and Gene Expression in Insects
- Mercury impact and mitigation studies
- Microbial Community Ecology and Physiology
- Enzyme Structure and Function
- Metal complexes synthesis and properties
- Remote-Sensing Image Classification
- Remote Sensing and Land Use
- Technology and Security Systems
- Wireless Sensor Networks and IoT
- Ideological and Political Education
- Water Quality Monitoring and Analysis
- Gas Sensing Nanomaterials and Sensors
- Food Quality and Safety Studies
- Methane Hydrates and Related Phenomena
China Southern Power Grid (China)
2024
University of Washington
2019-2024
Washington University in St. Louis
2012-2022
Wuhan University
1999-2020
Peking University
2010-2015
Fudan University
2010
Yangtze Normal University
2008
Institute of Scientific and Technical Information
2007
China Geological Survey
2004-2005
Kao Yuan University
2004
The rapid increase of the potent greenhouse gas methane in atmosphere creates great urgency to develop and deploy technologies for mitigation. One approach removing is use bacteria which their carbon energy source (methanotrophs). Such naturally convert CO 2 biomass, a value-added product cobenefit removal. Typically, methanotrophs grow best at around 5,000 10,000 ppm methane, but 1.9 ppm. Air above emission sites such as landfills, anaerobic digestor effluents, rice paddy oil wells contains...
We engineered a fatty acid overproducing Escherichia coli strain through overexpressing tesA (“pull”) and fadR (“push”) knocking out fadE (“block”). This “pull-push-block” strategy yielded 0.17 g of acids (C12–C18) per gram glucose (equivalent to 48% the maximum theoretical yield) in batch cultures during exponential growth phase under aerobic conditions. Metabolic fluxes were determined for E. its control using tracer ([1,2-13C]glucose) experiments 13C-metabolic flux analysis. Cofactor...
Synechococcus elongatus UTEX 2973 is the fastest growing cyanobacterium characterized to date. Its genome was found be 99.8% identical S. 7942 yet it grows twice as fast. Current genome-to-phenome mapping still poorly performed for non-model organisms. Even species with genomes, cell phenotypes can strikingly different. To understand 2973's fast-growth phenotype and its metabolic features advantageous photo-biorefineries, 13C isotopically nonstationary flux analysis (INST-MFA), biomass...
Sphingobium sp. SYK-6 is a soil bacterium boasting well-studied ligninolytic pathway and the potential for development into microbial chassis lignin valorization. An improved understanding of its metabolism will help researchers in engineering production value-added chemicals through We used 13C-fingerprinting, 13C metabolic flux analysis (13C-MFA), RNA-sequencing differential expression to uncover following traits: (i) prefers alkaline conditions, making it an efficient host consolidated...
Synthetic biology aspires to develop frameworks that enable the construction of complex and reliable gene networks with predictable functionalities. A key limitation is increasing network complexity increases demand for cellular resources, potentially causing resource-associated interference among noninteracting circuits. Although recent studies have shown effects resource competition on circuit behaviors, mechanisms decouple such remain unclear. Here, we constructed three systems in...
Synechocystis sp. strain PCC 6803 has been widely used as a photo-biorefinery chassis. Based on its genome annotation, this species contains complete TCA cycle, an Embden-Meyerhof-Parnas pathway (EMPP), oxidative pentose phosphate (OPPP), and Entner-Doudoroff (EDP). To evaluate how catabolizes glucose under heterotrophic conditions, we performed 13 C metabolic flux analysis, metabolite pool size gene knockouts, heterologous expressions. The results revealed cyclic mode of through the OPPP....
The central carbon metabolism of cyanobacteria is under debate. For over 50 years, the lack α-ketoglutarate dehydrogenase has led to belief that have an incomplete TCA cycle. Recent in vitro enzymatic experiments suggest this cycle may fact be closed. current study employed (13) C isotopomers delineate pathways cyanobacterium Synechocystis sp. PCC 6803. By tracing incorporation supplemented glutamate into downstream metabolites cycle, we observed a direct vivo transformation succinate....
ABSTRACT This study investigated metabolic responses in Synechocystis sp. strain PCC 6803 to photosynthetic impairment. We used 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU; a photosystem II inhibitor) block O 2 evolution and ATP/NADPH generation by linear electron flow. Based on 13 C-metabolic flux analysis ( C-MFA) RNA sequencing, we have found that employs unique photoheterotrophic metabolism. First, glucose catabolism forms cyclic route includes the oxidative pentose phosphate (OPP)...
In ancient Chinese philosophy, Yin-Yang describes two contrary forces that are interconnected and interdependent. This concept also holds true in microbial cell factories, where Yin represents energy metabolism the form of ATP, Yang carbon metabolism. Current biotechnology can effectively edit genome or introduce novel enzymes to redirect fluxes. On other hand, loses significant free as heat when converting sugar into ATP; while maintenance expenditures further aggravate ATP shortage. The...
Abstract Background Flux analyses, including flux balance analysis (FBA) and 13 C-metabolic ( C-MFA), offer direct insights into cell metabolism, have been widely used to characterize model non-model microbial species. Nonetheless, constructing the C-MFA performing calculation are demanding for new learners, because they require knowledge of metabolic networks, carbon transitions, computer programming. To facilitate standardize modeling work, we set out publish a user-friendly...
The microalga Chlorella sorokiniana has attracted much attention for lipid production and wastewater treatment. It can perform photosynthesis organic carbon utilization concurrently. To understand its phototrophic metabolism, a biomass compositional analysis, 13C metabolic flux metabolite pool size analyses were performed. Under dark condition, the oxidative pentose phosphate pathway (OPP) was major route glucose catabolism (88% flux) cyclic OPP–glycolytic formed. light fluxes in catabolism,...
This study explores the impact and implementation strategies of digital transformation in educational management, with a focus on higher education institutions under LLDIKTI Region 2, using Universitas PGRI Palembang as case study. Digital management encompasses administrative processes, learning systems, student services, leveraging technologies like Learning Management Systems (LMS), big data, artificial intelligence (AI). The research identifies key challenges such limited infrastructure,...
Methylomicrobium buryatense 5GB1 is an obligate methylotroph which grows on methane or methanol with similar growth rates. It has long been assumed that the core metabolic pathways must be two substrates, but recent studies of metabolism in this bacterium suggest might have significant differences from methane. In study, both a targeted metabolomics approach and 13C tracer were taken to understand carbon M. during determine whether such occur. Our results systematic shift active increased...
Methanotrophic bacteria are a group of prokaryotes capable using methane as their sole carbon and energy source. Although efforts have been made to simulate elucidate metabolism via computational approaches or 13C tracer analysis, major gaps still exist in our understanding methanotrophic at the systems level. Particularly, direct measurements system-wide fluxes required understand metabolic network function. Here, we quantified central type I methanotroph, "Methylotuvimicrobium buryatense"...
Methylotuvimicrobium buryatense 5GB1C, a fast-growing gammaproteobacterial methanotroph, is equipped with two glycolytic pathways, the Entner-Doudoroff (ED) pathway and Embden-Meyerhof-Parnas (EMP) pathway. Metabolic flux analysis 13C-labeling experiments have shown EMP principal route in M. while ED appears to be metabolically energetically insignificant. However, it has not been possible obtain null mutant edd-eda genes encoding unique enzymatic reactions pathway, suggesting may essential...