A5043206163- Microbial Natural Products and Biosynthesis
- Microbial Metabolic Engineering and Bioproduction
- Genomics and Phylogenetic Studies
- Microbial Metabolism and Applications
- Fungal Biology and Applications
- Plant biochemistry and biosynthesis
- Microbial metabolism and enzyme function
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Chemical Synthesis and Analysis
- Synthetic Organic Chemistry Methods
- Photosynthetic Processes and Mechanisms
- RNA and protein synthesis mechanisms
- Carbohydrate Chemistry and Synthesis
- Marine Sponges and Natural Products
- Phytochemistry and Bioactivity Studies
- Synthesis and biological activity
- Phytochemical compounds biological activities
- Chemotherapy-induced cardiotoxicity and mitigation
- Legume Nitrogen Fixing Symbiosis
- Protein Hydrolysis and Bioactive Peptides
- Click Chemistry and Applications
- Pharmacogenetics and Drug Metabolism
- Natural product bioactivities and synthesis
- Catalysis for Biomass Conversion
Qingdao Agricultural University
2015-2025
TU Dresden
2021-2024
Qingdao Center of Resource Chemistry and New Materials
2019
South China Sea Institute Of Oceanology
2011-2015
Institute of Oceanology
2011-2015
Chinese Academy of Sciences
2011-2015
University of Chinese Academy of Sciences
2011-2012
Natural products containing the tetramic acid core scaffold have been isolated from an assortment of terrestrial and marine species often display wide ranging potent biological activities including antibacterial, antiviral antitumoral activities.
Bioactivity screening revealed that the antifungal activities of EtOAc extracts from coculture broths Trametes versicolor SY630 with either Vanderbylia robiniophila SY341 or Ganoderma gibbosum SY1001 were significantly improved compared to monocultures. Activity-guided isolation led discovery five aromatic compounds (1–5) broth T. and V. two sphingolipids (6 7) G. SY1001. Tramevandins A–C (1–3) 17-ene-1-deoxyPS (6) are new compounds, while 1-deoxyPS (7) is a natural product. Notably,...
Three new pyridine derivatives, irpelactedines A–C (1–3), and a furan derivative, irpelactedine D (5), along with two structurally related known compounds, irpexidine A (4) 5‐carboxy‐2‐furanpropanoic acid (6), were isolated from the medicinal fungus Irpex lacteus SY1002. Their structures elucidated through NMR mass spectral analyses, combined density functional theory calculations of ECD data. Evaluation angiotensin‐converting enzyme (ACE) inhibitory activity revealed that compounds 1 3...
Bioinformatic analyses indicate that TrdC, SlgL, LipX2, KirHI, and FacHI belong to a group of highly homologous proteins involved in biosynthesis actinomycete-derived tirandamycin B, streptolydigin, α-lipomycin, kirromycin, factumycin, respectively. However, assignment their biosynthetic roles has remained elusive. Gene inactivation complementation, vitro biochemical assays with synthetic analogues, point mutations, phylogenetic tree reveal these represent new family Dieckmann cyclases drive...
Abstract The proficiency of phyllosphere microbiomes in efficiently utilizing plant-provided nutrients is pivotal for their successful colonization plants. methylotrophic capabilities Methylobacterium/Methylorubrum play a crucial role this process. However, the precise mechanisms facilitating efficient remain elusive. In present study, we investigate significance methanol assimilation shaping success mutualistic relationships between methylotrophs and A set strains originating from...
Butadiene is a platform chemical used as an industrial feedstock for the manufacture of automobile tires, synthetic resins, latex and engineering plastics. Currently, butadiene predominantly synthesized byproduct ethylene production from non-renewable petroleum resources. Although idea biological synthesis sugars has been discussed in literature, success that goal so far not reported. As model system methanol assimilation, Methylobacterium extorquens AM1 can produce several unique metabolic...
Candida albicans and Cryptococcus neoformans, human-pathogenic fungi found worldwide, are receiving increasing attention due to high morbidity mortality in immunocompromised patients. In the present work, 110 fungus pairs were constructed by coculturing 16 wood-decaying basidiomycetes, among which coculture of Trametes robiniophila Murr Pleurotus ostreatus was strongly inhibit pathogenic through bioactivity-guided assays. A combination metabolomics molecular network analysis revealed that 44...
TrdL, encoding a flavin-dependent oxidoreductase in the tirandamycin gene cluster, was inactivated to afford ΔtrdL mutant, fermentation of which yielded new intermediate, E (5), and an additional early F (6), if XAD-16 resin introduced. TrdL overexpressed E. coli, protein shown efficiently catalyze transformations from 5 A (1) 6 D (4), demonstrating its function as 10-hydroxy dehydrogenase.
The tirandamycins (TAMs) are a small group of Streptomyces-derived natural products that target bacterial RNA polymerase. Within the TAM biosynthetic cluster, trdE encodes glycoside hydrolase whose role in biosynthesis has been undefined until now. We report vivo inactivation leads to accumulation pre-tirandamycin, earliest intermediate released from its mixed polyketide/nonribosomal peptide assembly line. In vitro and site-directed mutagenesis studies showed TrdE, putative hydrolase,...
In our previous work, postredienes A-C, three unusual linear sesterterpenes with high antifungal activities, were isolated from Pleurotus ostreatus SY10 when cocultured Trametes robiniophila SY636. However, their titers low, and exploration of newly biosynthesized trace analogues is required. Herein, genome mining analysis predicted that 17 gene clusters are involved in terpenoid biosynthesis P. ostreatus. Thus, coculture conditions for strains SY636 optimized using a single-factor test...
Nocamycins I and II, produced by the rare actinomycete Saccharothrix syringae, belong to tetramic acid family natural products. show potent antimicrobial activity they hold great potential for antibacterial agent design. However, up now, little is known about exact biosynthetic mechanism of nocamycin.
The C7 (C9 or C10)-O-l-rhodosamine-bearing anthracycline antibiotic cytorhodins and their biosynthetic intermediates were recently isolated from Streptomyces sp. SCSIO 1666. Cosmid p17C4 the lydicus genomic library, which harbors both genes for l-rhodinose (or 2-deoxy-l-fucose) its glycosyltransferase (encoded by slgG), was introduced into 1666 to yield recombinant strain 1666/17C4. Chemical investigations of this strain's secondary metabolic potential revealed production different...
Methylobacterium species, the representative bacteria distributed in phyllosphere region of plants, often synthesize carotenoids to resist harmful UV radiations. extorquens is known produce a carotenoid pigment and recent research revealed that this has C30 backbone. However, its exact structure remains unknown. In present study, produced by M. AM1 was isolated determined as 4-[2-O-11Z-octadecenoyl-β-glucopyranosyl]-4,4'-diapolycopenedioc acid (1), glycosylated carotenoid. Furthermore, genes...
A Gram-stain-negative, aerobic, yellow-pigmented, non-flagellated, non-gliding, oxidase- and catalase-positive bacterium, designated CY01T, was isolated from seawater of the Yellow Sea. CY01T grew at 15-37 °C (optimum, 30 °C), pH 5-8 6.5-7.5) with 0.5-12 % (w/v) NaCl 0.5-3.5 %). It could not produce flexirubin-type pigment or reduce nitrate to nitrite. showed highest 16S rRNA gene sequence similarity type strain Euzebyella saccharophila (97.0 %) clustered tightly species genus in...
Abstract Background Methylorubrum extorquens AM1 can be engineered to convert methanol value‐added chemicals. Most of these chemicals derive from acetyl‐CoA involved in the serine cycle. However, recent studies on methylotrophic metabolism have suggested that C3 pyruvate is a good potential precursor for broadening types synthesized products. Methods and Results In present study, we found isobutanol was model chemical could generated through 2‐keto acid pathway. Initially, M. only produce...
Anthracycline antitumor cytorhodins X and Y feature a rare 9α-glycoside 7-dexoy-aglycone. Characterization of the cytorhodin gene cluster from Streptomyces sp. SCSIO 1666 through inactivations metabolite analyses reveals three glycosyltransferases (GTs) involved in sugar tailoring steps. The duo CytG1 CytL effects C-7 glycosylation with l-rhodosamine whereas iterative GT CytG3 CytW similarly modifies both C-9 C-10 positions. CytG2 also acts iteratively by incorporating second third moiety...
Abstract Antibiotic-producing microorganism can develop strategies to deal with self-toxicity. Cytorhodins X and Y, cosmomycins A B, iremycin, are produced as final products from a marine-derived Streptomyces sp. SCSIO 1666. These C-7 reduced metabolites show antimicrobial comparable cytotoxic activities relative their glycosylated counterparts. However, the biosynthetic mechanisms relevant enzymes that drive reduction in cytorhodin biosynthesis have not yet been characterized. Here we...
Nocamycins I and II, featured with a tetramic acid scaffold, were isolated from the broth of Saccharothrix syringae NRRL B-16468. The biosynthesis nocamycin require an intermediate bearing hydroxyl group at C-10 position. A short chain dehydrogenase/reductase NcmD was proposed to catalyze conversion ketone By using λ-RED recombination technology, we generated deletion mutant strain S. MoS-1005, which produced new F We then overexpressed in Escherichia coli BL21 (DE3), purified His 6 -tagged...