Handling clustering problems are important in data statistics, pattern recognition and image processing. The mean-shift algorithm, a common unsupervised algorithms, is widely used to solve problems. However, the algorithm restricted by its huge computational resource cost. In previous research [1], we proposed novel GPU-accelerated Faster Mean-shift which greatly speed up cosine-embedding problem. this study, extend improve handle Euclidean distance metrics. Different from conventional...

The world faces the challenge to develop sustainable technologies replace thousands of products that have been generated from fossil fuels. Microbial cell factories serve as promising alternatives for production diverse commodity chemicals and biofuels renewable resources. For example, polylactic acid (PLA) with its biodegradable properties is a sustainable, environmentally friendly alternative polyethylene. At present, PLA microbial mainly dependent on food crops such corn sugarcane....

The effect of the electric field on conformational properties protein 1BBL was investigated by molecular dynamics simulations. Our simulation results clearly capture structural transitions sample from helix to turn or random coil conformation induced increasing strength field. During our analysis, we found that stability is weakened, and stretched as an unfolded structure when it exposed in a sufficiently high characteristic time jump occurs evolution curves root mean square deviation (RMSD)...

Synechocystis sp. strain PCC 6803 has been widely used as a photo-biorefinery chassis. Based on its genome annotation, this species contains complete TCA cycle, an Embden-Meyerhof-Parnas pathway (EMPP), oxidative pentose phosphate (OPPP), and Entner-Doudoroff (EDP). To evaluate how catabolizes glucose under heterotrophic conditions, we performed 13 C metabolic flux analysis, metabolite pool size gene knockouts, heterologous expressions. The results revealed cyclic mode of through the OPPP....

The central carbon metabolism of cyanobacteria is under debate. For over 50 years, the lack α-ketoglutarate dehydrogenase has led to belief that have an incomplete TCA cycle. Recent in vitro enzymatic experiments suggest this cycle may fact be closed. current study employed (13) C isotopomers delineate pathways cyanobacterium Synechocystis sp. PCC 6803. By tracing incorporation supplemented glutamate into downstream metabolites cycle, we observed a direct vivo transformation succinate....

Polyamide (PA) semipermeable membranes typically used for reverse osmosis water treatment processes are prone to fouling, which reduces the amount and quality of produced. By synergistically coupling photothermal bactericidal properties graphene oxide (GO) nanosheets, gold nanostars (AuNS), hydrophilic polyethylene glycol (PEG) on PA membrane surfaces, we have dramatically improved fouling resistance these membranes. Batch experiments from three classes presented: mineral scaling (CaCO3...

In this article, we devote to solve the consensus problem for nonlinear multiagent system under energy consumption constraint. A novel control protocol which combines impulse with event-triggered mechanism is proposed. By setting event decision time at pulse time, it can not only reduce triggered times but also avoid occurrence of "Zeno behavior." order conservatism protocol, input saturation constraint and state are both discussed, limited receiving channel agent sampling range sampler,...

The impulsive consensus problems of nonlinear time-delay multiagent system under communication constraints are analyzed and investigated in this article. In view the limited channel, global input saturation constraint is integrated into design impulse control protocol. Furthermore, order to reduce energy consumption cost, two kinds protocols designed that conclude general event-triggered protocols. For protocols, by setting event decision time at time, novel not only decrease frequency but...

ABSTRACT This study investigated metabolic responses in Synechocystis sp. strain PCC 6803 to photosynthetic impairment. We used 3-(3,4-dichlorophenyl)-1,1-dimethylurea (DCMU; a photosystem II inhibitor) block O 2 evolution and ATP/NADPH generation by linear electron flow. Based on 13 C-metabolic flux analysis ( C-MFA) RNA sequencing, we have found that employs unique photoheterotrophic metabolism. First, glucose catabolism forms cyclic route includes the oxidative pentose phosphate (OPP)...

The paper mainly investigates the synchronization problem of coupled stochastic reaction-diffusion neural networks with multiple weights and time delays via pinning impulsive control. Based on analysis theory, inequality technology, LMIs, principle comparison, sufficient conditions are obtained to ensure RDNNs using a suitable control protocol. Compared recent relevant theoretical research, model established in this is more universal, takes industrial constraints into consideration. In...

Microbes have complex metabolic pathways that can be investigated using biochemistry and functional genomics methods. One important technique to examine cell central metabolism discover new enzymes is 13C-assisted analysis 1. This based on isotopic labeling, whereby microbes are fed with a 13C labeled substrates. By tracing the atom transition paths between metabolites in biochemical network, we determine enzymes. As complementary method transcriptomics proteomics, approaches for...

The applications of stable isotopes in metabolomics have facilitated the study cell metabolisms. Stable isotope-assisted requires: (1) properly designed tracer experiments; (2) stringent sampling and quenching protocols to minimize isotopic alternations; (3) efficient metabolite separations; (4) high resolution mass spectrometry resolve overlapping peaks background noises; (5) data analysis methods databases decipher clusters over a broad m/z range (mass-to-charge ratio). This paper...

In this article, the consensus problem of nonlinear multiagent systems with grouping via state-constraint impulsive protocols is investigated. Two types cases and without leader agent are studied by using two kinds protocols. A judgement strategy designed to decide group in systems, control protocols, which include partial state constraint full constraint, proposed make system cut down cost communication reduce irreversible damage equipment. Then, based on algebraic graph theory, Lyapunov...

This study used 13C tracers and dynamic labeling to reveal metabolic features (nutrients requirements, pathway delineation metabolite turnover rates) of Clostridium carboxidivorans P7, a model strain for industrial syngas fermentation, its implication with bioreactor mass transfer. P7 shows poor activity synthesizing amino acids (e.g., phenylalanine) thus, needs rich medium cell growth. The has multiple carbon fixation routes (Wood-Ljungdahl pathway, pyruvate:ferredoxin oxidoreductase...

In this article, the consensus problem of time-delay fuzzy multiagent systems (TDFMASs) with saturation-constraint impulsive control is considered. To better meet complex requirements real-world models, a nonlinear model TDFMASs and corresponding rules are designed. Then, in view limited communication channel, all agents channel constraint partial considered control, respectively. Furthermore, for sake increasing antiinterference agents, hybrid impulse protocol addition, help Lyapunov...

The Zymomonas mobilis ZM4 strain with excellent ethanol‐producing capabilities was the first of Z. , which sequenced. This is resistant to transformation, and no previous study has shown a detailed protocol for electrotransfer foreign DNA. In this work, many electrical biological parameters were selected evaluated in order optimize electrotransformation ZM4. First, improved transformation efficiencies 11 896, 99, 96 5989 transformants/μg DNA separately achieved shuttle plasmid pZB21‐mini...