A5103248984- Memory and Neural Mechanisms
- Neuroscience and Neuropharmacology Research
- Neural dynamics and brain function
- Photoreceptor and optogenetics research
- Spatial Cognition and Navigation
- Olfactory and Sensory Function Studies
- Neuroscience and Neural Engineering
- Robotics and Sensor-Based Localization
- Child and Animal Learning Development
- Diffusion and Search Dynamics
- Neurogenesis and neuroplasticity mechanisms
- Sleep and Wakefulness Research
- Advanced Memory and Neural Computing
- Ultrasound and Hyperthermia Applications
- Biocrusts and Microbial Ecology
- Zebrafish Biomedical Research Applications
- Stress Responses and Cortisol
- Advanced MRI Techniques and Applications
- Advanced Neuroimaging Techniques and Applications
- Neurobiology and Insect Physiology Research
- Morphological variations and asymmetry
- Vestibular and auditory disorders
- Neuroendocrine regulation and behavior
- 3D Printing in Biomedical Research
- Medical and Biological Ozone Research
Korea Institute of Science and Technology
2012-2024
Korea University of Science and Technology
2017-2024
Bio-Medical Science (South Korea)
2019-2024
Korea Institute of Brain Science
2020-2024
University of Science and Technology
2017
Howard Hughes Medical Institute
2010-2012
Center for Molecular Medicine and Immunology
2012
Janelia Research Campus
2010-2012
Rutgers, The State University of New Jersey
2002-2012
Université Laval
1999-2001
Although anatomical, lesion, and imaging studies of the hippocampus indicate qualitatively different information processing along its septo-temporal axis, physiological mechanisms supporting such distinction are missing. We found fundamental differences between dorsal (dCA3) ventral-most parts (vCA3) in both environmental representation temporal dynamics. Discrete place fields dCA3 neurons evenly covered all testing environments. In contrast, vCA3 (1) rarely showed continuous two-dimensional...
A topographical relationship exists between the hippocampus-entorhinal cortex and neocortex. However, it is not known how these anatomical connections are utilized during information exchange behavior. We recorded theta oscillations along entire extent of septotemporal axis hippocampal CA1 pyramidal layer. While frequency oscillation remained same long axis, amplitude coherence recording sites decreased from dorsal to ventral hippocampus (VH). Theta phase shifted monotonically with distance...
Abstract The CA3 and CA1 pyramidal neurons are the major principal cell types of hippocampus proper. strongly recurrent collateral system cells largely parallel‐organized suggest that these regions perform distinct computations. However, a comprehensive comparison between in terms firing properties, network dynamics, behavioral correlations is sparse intact animal. We performed large‐scale recordings dorsal rats to quantify similarities differences ( n > 3,600) 2,200) during sleep...
The central amygdaloid nucleus projects to brainstem and hypothalamic nuclei mediating fear responses receives convergent sensory inputs from the basolateral complex. However, interposed between complex is a string of interconnected GABAergic cell clusters, intercalated masses. Here, we analyzed how neurons influence impulse traffic using whole-cell recordings, microstimulation, local application glutamate receptor antagonists in brain slices. Our results suggest that receive glutamatergic...
Abstract Recordings of large neuronal ensembles and neural stimulation high spatial temporal precision are important requisites for studying the real‐time dynamics networks. Multiple‐shank silicon probes enable large‐scale monitoring individual neurons. Optical genetically targeted neurons expressing light‐sensitive channels or other fast (milliseconds) actuators offers means controlled perturbation local circuits. Here we describe a method to equip shanks with micron‐scale light guides...
Driven either by external landmarks or internal dynamics, hippocampal neurons form sequences of cell assemblies. The coordinated firing these active cells is organized the prominent “theta” oscillations in local field potential (LFP): place discharge at progressively earlier theta phases as rat crosses respective (“phase precession”). faster oscillation frequency and slower LFP, underlying phase precession, creates a paradox. How can oscillating comprise population oscillation, reflected...
Abstract Environmental cues affect place cells responses, but whether this information is integrated versus segregated in distinct hippocampal cell populations unclear. Here, we show that, mice running on a treadmill enriched with visual-tactile landmarks, are more strongly controlled by landmark-associated sensory inputs deeper regions of CA1 pyramidal layer (CA1d). Many CA1d display several firing fields correlated mapping positions slightly before or within the landmarks. Supporting...
A major challenge in neuroscience is linking behavior to the collective activity of neural assemblies. Understanding input-output relationships neurons and circuits requires methods with spatial selectivity temporal resolution appropriate for mechanistic analysis ensembles behaving animal, i.e. recording representatively large samples isolated single neurons. Ensemble monitoring neuronal has progressed remarkably past decade both small large-brained animals, including human subjects1-11....
Spatial location in the environment can be defined relation to specific landmarks or global context, and is estimated from both sensing of inner sense cumulated locomotion referred as path-integration. The respective contribution landmark path-integration place-cell activity hippocampus still unclear complicated by fact that two mechanisms usually overlap. To bias spatial toward path-integration, we use a treadmill equipped with long belt on which male mice run sequentially through zone...
Abstract Place cells exhibit spatially selective firing fields that collectively map the continuum of positions in environments; how such activity pattern develops with experience is largely unknown. Here, we record putative granule (GCs) and mossy (MCs) from dentate gyrus (DG) over 27 days as mice repetitively run through a sequence objects fixed onto treadmill belt. We observe progressive transformation GC spatial representations, sparse encoding object locations patterns to increasingly...
The intercalated (ITC) cell masses are small GABAergic clusters interposed between the basolateral (BL) complex and central (CE) nucleus of amygdala. ITC cells receive excitatory afferents from BL generate feed-forward inhibition in CE nucleus. Recently it was shown that could gate impulse traffic a spatiotemporally differentiated manner. In addition, hypothesized lateromedial inhibitory interactions different played critical role this respect. Given potential importance such conditional...
Silicon probes are multisite electrodes used for the electrophysiological recording of large neuronal ensembles. Optoelectronic (OEPs) recent upgrades that allow, in parallel, delivery local optical stimuli. The procedures to use these delicate chronic experiments mice still underdeveloped and typically assume one-time uses. Here, we developed a micro-drive, support OEPs fibers, hat enclosure, which fabrications consist fitting fastening together plastic parts made with 3D printers....
The dentate gyrus (DG) is critical for detecting changes in environments; however, how granule cells (GCs) and mossy (MCs), the two excitatory cell types of DG, respond to small object layout unclear. Here, we recorded GCs MCs, identified by spike feature optogenetic tagging, as mice ran on a treadmill belt enriched with visual-tactile cues. We observed that fixing new cue induced reconfiguration GC MC spatial representations via emergence, extinction rate alteration firing fields. For both...
Ischemic damage after stroke disrupts the complex balance of inhibitory and excitatory activity within cortical network causing brain functional asymmetry. Cerebellar deep nuclei with its extensive projections to regions could be a prospective target for stimulation restore inter-hemispheric enhance neural plasticity stroke. In our study, we repeatedly stimulated lateral cerebellar nucleus (LCN) by low-intensity focused ultrasound (LIFU) 3 days rehabilitation middle cerebral artery occlusion...
Extracellular recordings in freely moving animals allow the monitoring of brain activity from populations neurons at single-spike temporal resolution. While state-of-the-art electrophysiological recording devices have been developed recent years (e.g., µLED and Neuropixels silicon probes), implantation methods for probes rats mice not advanced substantially a decade. The surgery is complex, takes time to master, involves handling expensive valuable animal subjects. In addition, chronic...
A major challenge in neuroscience is linking behavior to the collective activity of neural assemblies. Understanding input-output relationships neurons and circuits requires methods with spatial selectivity temporal resolution appropriate for mechanistic analysis ensembles behaving animal, i.e. recording representatively large samples isolated single neurons. Ensemble monitoring neuronal has progressed remarkably past decade both small large-brained animals, including human subjects1-11....
The perirhinal area is a rostrocaudally oriented strip of cortex in which lesions produce memory and perceptual impairments. It receives topographically organized transverse projections from associative neocortical areas endowed with intrinsic longitudinal connections that could distribute inputs the rostrocaudal axis. In search distinguishing network properties might support involvement memory, we have performed whole-cell recordings horizontal slices preserved links connections....
Using the highly plastic synapses between mechanoreceptor sensory neurons and siphon motor of Aplysia as a model, we have investigated whether switching off on individual synaptic release sites is strategy that used by in forms short-term modulation with time course minutes to hours. We modified some techniques classical quantal analysis examined kinetics depression under different stimulation protocols answer this question. Our shows both caused homosynaptic activity facilitation induced an...