A5019896022- CRISPR and Genetic Engineering
- Pluripotent Stem Cells Research
- RNA Interference and Gene Delivery
- RNA and protein synthesis mechanisms
- Viral Infections and Vectors
- Plant-Microbe Interactions and Immunity
- Animal Genetics and Reproduction
- RNA Research and Splicing
- Viral Infections and Outbreaks Research
- Advanced biosensing and bioanalysis techniques
- Plant Pathogens and Resistance
- Plant Disease Resistance and Genetics
- Nuclear Receptors and Signaling
- Legume Nitrogen Fixing Symbiosis
- Plant Molecular Biology Research
- Single-cell and spatial transcriptomics
- Mosquito-borne diseases and control
- Virus-based gene therapy research
- Soybean genetics and cultivation
- Cellular transport and secretion
- Helminth infection and control
- Microbial Metabolites in Food Biotechnology
- Plant Gene Expression Analysis
- Coccidia and coccidiosis research
- Plant Parasitism and Resistance
Washington University in St. Louis
2018-2025
James S. McDonnell Foundation
2024
Nanjing Agricultural University
2014-2022
Yebio Bioengineering (China)
2021
Jiangsu Academy of Agricultural Sciences
2019
Shanghai Veterinary Research Institute
2016-2018
Horizon Discovery Group (United States)
2016-2017
Chinese Academy of Agricultural Sciences
2016
Fraunhofer USA
2016
The University of Texas at Austin
2004-2015
Targeted gene disruption in rats paves the way for new human disease models.
Isogenic settings are routine in model organisms, yet remain elusive for genetic experiments on human cells. We describe the use of designed zinc finger nucleases (ZFNs) efficient transgenesis without drug selection into PPP1R12C gene, a “safe harbor” locus known as AAVS1 . ZFNs enable targeted at frequency up to 15% following transient transfection both transformed and primary cells, including fibroblasts hES When added this locus, transgenes such expression cassettes shRNAs,...
Homologous recombination-based gene targeting using Mus musculus embryonic stem cells has greatly impacted biomedical research. This study presents a powerful new technology for more efficient and less time-consuming in mice injection of zinc-finger nucleases (ZFNs), which generate site-specific double strand breaks, leading to insertions or deletions via DNA repair by the nonhomologous end joining pathway. Three individual genes, multidrug resistant 1a (Mdr1a), jagged 1 (Jag1), notch...
Recessively inherited loss-of-function mutations in the PTEN-induced putative kinase 1(Pink1), DJ-1 (Park7) and Parkin (Park2) genes are linked to familial cases of early-onset Parkinson's disease (PD). As part its strategy provide more tools for research community, The Michael J. Fox Foundation Research (MJFF) funded generation novel rat models with targeted disruption ofPink1, or determined if loss these proteins would result a progressive PD-like phenotype. Pathological, neurochemical...
The rapid development of CRISPR technology greatly impacts the field genetic engineering. simplicity in design and generation highly efficient reagents allows more researchers to take on genome editing different model systems their own labs, even for those who found it daunting before. An active complex contains a protein component (Cas9) an RNA (small guide [sgRNA]), which can be delivered into cells various formats. Cas9 introduced as DNA expression plasmid, vitro transcripts, or...
Animal models are critical for gaining insights into autism spectrum disorder (ASD). Despite their apparent advantages to mice neural studies, rats have not been widely used disorders of the human CNS, such as ASD, lack convenient genome manipulation tools. Here we describe two first transgenic rat developed using zinc-finger nuclease (ZFN) methodologies, and initial behavioral assessment a rapid juvenile test battery. A syndromic nonsyndromic model ASD were created separate knockout lines...
The cytochrome P450 monooxygenases (P450s) represent a large and important enzyme superfamily in plants. They catalyze numerous monooxygenation/hydroxylation reactions biochemical pathways, P450s are involved variety of metabolic pathways participate the homeostasis phytohormones. CYP82 family genes specifically reside dicots usually induced by distinct environmental stresses. However, their functions largely unknown, especially soybean (Glycine max L.). Here, we report function GmCYP82A3,...
Yeast phosphatidylinositol transfer protein (Sec14p) is essential for Golgi function and cell viability. We now report a characterization of five yeast SFH (Sec Fourteen Homologue) proteins that share 24–65% primary sequence identity with Sec14p. show Sfh1p, which shares 64% Sec14p, nonfunctional as Sec14p in vivo or vitro. Yet,SFH sharing low similarity (i.e., Sfh2p, Sfh3p, Sfh4p, Sfh5p) represent novel (PITPs) exhibit phosphatidylinositol- but not phosphatidylcholine-transfer activity...
The WRKY proteins are a superfamily of transcription factors and members play essential roles in the modulation diverse physiological processes, such as growth, development, senescence response to biotic abiotic stresses. However, biological majority family remains poorly understood soybean relative research progress model plants.In this study, we identified characterized GmWRKY40, which is group IIc gene. Transient expression analysis revealed that GmWRKY40 protein located nucleus plant...
Oropouche orthobunyavirus (OROV; Peribunyaviridae ) is a mosquito-transmitted virus that causes widespread human febrile illness in South America, with occasional progression to neurologic effects. Host factors mediating the cellular entry of OROV are undefined. Here, we show uses host protein low-density lipoprotein–related 1 (Lrp1) for efficient infection. Cells from evolutionarily distinct species lacking Lrp1 were less permissive infection than cells Lrp1. Treatment either high-affinity...
Previous genomic efforts on chromosome 9p deletion and duplication syndromes have utilized low resolution strategies (i.e., karyotypes, microarrays). We present the first large-scale whole-genome sequencing (WGS) study of 100 individuals from families with 9p-related including 85 unrelated probands through 9P-ARCH (Advanced Research in Chromosomal Health: Genomic, Phenotypic, Functional Aspects 9p-Related syndromes) research network. analyzed architecture these syndromes, highlighting...
Microneme proteins play an important role in the adherence of apicomplexan parasites to host cells during invasion process. In this study, microneme 2 protein from protozoan parasite Eimeria tenella (EtMIC2) was cloned, characterized, and its protective efficacy as a DNA vaccine investigated. The EtMIC2 gene, which codes for 35.07 kDa E. sporulated oocysts, cloned recombinant (rEtMIC2) produced Escherichia coli expression system. Immunostaining with anti-rEtMIC2 antibody showed that mainly...
Phosphatidylinositol transfer proteins (PITPs) regulate the interface between signal transduction, membrane-trafficking, and lipid metabolic pathways in eukaryotic cells. The best characterized mammalian PITPs are PITP alpha beta, two highly homologous that encoded by distinct genes. Insights into beta function systems have been gleaned exclusively from cell-free or permeabilized cell reconstitution resolution studies. Herein, we report for first time use of genetic approaches to directly...
Targeted gene mutation in the mouse is a primary strategy to understand function and relation phenotype. The Knockout Mouse Project (KOMP) had an initial goal develop public resource of embryonic stem (ES) cell clones that carry null mutations all genes. Indeed, many useful novel models have been generated from publically accessible targeted ES lines. However, there are limitations, including incorrect targeting or cassette structure, difficulties with germline transmission allele chimeric...