A5046131172- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Enzyme Catalysis and Immobilization
- Enzyme Structure and Function
- Microbial Metabolic Engineering and Bioproduction
- Natural Language Processing Techniques
- Topic Modeling
- Genomics and Phylogenetic Studies
- Phytase and its Applications
- Microbial Metabolites in Food Biotechnology
- Photosynthetic Processes and Mechanisms
- Bacteriophages and microbial interactions
- Microbial bioremediation and biosurfactants
- Advanced Text Analysis Techniques
- Plant-Microbe Interactions and Immunity
- Service-Oriented Architecture and Web Services
- Microbial Community Ecology and Physiology
- Enzyme-mediated dye degradation
- thermodynamics and calorimetric analyses
- Biochemical and biochemical processes
- Studies on Chitinases and Chitosanases
- Physiological and biochemical adaptations
- Plant Disease Management Techniques
- Cassava research and cyanide
- Amino Acid Enzymes and Metabolism
Korea Polar Research Institute
2016-2025
Korea University of Science and Technology
2016-2025
Sungkyunkwan University
2023
Dong-Eui University
1997-2022
National Institute of Advanced Industrial Science and Technology
2007-2022
Korea University
2015-2016
Korea Institute of Ocean Science and Technology
2014
Hanyang University
2006-2013
Anyang University
2012
National Institute of Fisheries Science
2003-2009
Ascorbic acid (AsA) maintains redox homeostasis by scavenging reactive oxygen species from prokaryotes to eukaryotes, especially plants. The enzyme monodehydroascorbate reductase (MDHAR) regenerates AsA catalysing the reduction of monodehydroascorbate, using NADH or NADPH as an electron donor. detailed recycling mechanism MDHAR remains unclear due lack structural information. Here, we present crystal structures in presence cofactors, nicotinamide adenine dinucleotide (NAD+) and phosphate...
Abstract Dehydroascorbate reductase (DHAR) is a key enzyme involved in the recycling of ascorbate, which catalyses glutathione (GSH)-dependent reduction oxidized ascorbate (dehydroascorbate, DHA). As result, DHAR regenerates pool reduced and detoxifies reactive oxygen species (ROS). In previous experiments involving transgenic rice, we observed that overexpression enhanced grain yield biomass. Since structure not available, enzymatic mechanism well-understood remains poorly characterized. To...
A novel microbial esterase, EaEST, from a psychrophilic bacterium Exiguobacterium antarcticum B7, was identified and characterized. To our knowledge, this is the first report describing structural analysis biochemical characterization of an esterase isolated genus Exiguobacterium. Crystal structure determined at resolution 1.9 Å, showed that enzyme has canonical α/β hydrolase fold with α-helical cap domain catalytic triad consisting Ser96, Asp220, His248. Interestingly, active site EaEST...
A hyperthermophilic membrane-related β-1,4-endoglucanase (family 5, cellulase) of the archaeon Pyrococcus horikoshii was found to be capable hydrolysing cellulose at high temperatures. The cellulase has promise for applications in biomass utilization. To clarify its detailed function, we determined crystal structures mutants enzyme complex with either substrate or product ligands. We were able resolve different kinds 1.65–2.01 Å (1 Å=0.1 nm). structural analysis various mutant enzymes...
Glutamate decarboxylase (GAD) from the archaeon Pyrococcus horikoshii was successfully expressed and purified, with aim of developing a hyperthermostable GAD for industrial applications. Its biochemical properties were different those reported other GADs. The enzyme had broad substrate specificity, its optimum pH temperature 8.0 >97 °C.
We identified two antimicrobial peptides (AMPs) with similarity to moronecidin in Antarctic fishes. The characteristics of both AMPs were determined using as a control. Moronecidin, which was first isolated from hybrid striped bass, is highly salt-resistant, and possesses broad-spectrum activity against various microbes. moronecidin-like peptide Notothenia coriiceps exhibited narrower spectrum higher salt sensitivity than moronecidin. AMP Parachaenichthys charcoti similar moronecidin,...
Cold-active acetyl xylan esterases allow for reduced bioreactor heating costs in bioenergy production. Here, we isolated and characterized a cold-active esterase (PbAcE) from the psychrophilic soil microbe Paenibacillus sp. R4. The enzyme hydrolyzes glucose penta-acetate acetate, reversibly producing xylan, it shows higher activity at 4°C than 25°C. We solved crystal structure of PbAcE 2.1-Å resolution to investigate its active site reason low-temperature activity. Structural analysis showed...
Parochlus steinenii is the only flying insect native to Antarctica. To elucidate molecular mechanisms underlying its adaptation cold environments, we conducted comparative genomic analyses of P. and closely related lineages. In an analysis gene family evolution, 68 rapidly evolving families, involved in innate immune system, unfolded protein response, DNA packaging, folding, unsaturated fatty acid biosynthesis were detected. Some families steinenii-specific showed phylogenetic instability....
Parochlus steinenii is a winged midge from King George Island. It cold-tolerant and endures the harsh Antarctic winter. Previously, we reported genome of this midge, but assembly with short reads had limited contig contiguity, which reduced completeness annotated gene sets. Recently, contiguity has been increased using nanopore technology. A number methods for enhancing low base quality have reported, including long-read (e.g. Nanopolish) or short-read Pilon) based methods. Based on these...
S-Formylglutathione is hydrolyzed to glutathione and formate by an S-formylglutathione hydrolase (SFGH) (3.1.2.12). This thiol esterase belongs the family also known as D. SFGHs contain highly conserved active residues of Ser-Asp-His a catalytic triad at site. Characterization investigation SFGH from Antarctic organisms molecular level needed for industrial use through protein engineering.A novel cold-active (SfSFGH) Shewanella frigidimarina, composed 279 amino acids with mass ~ 31.0 kDa,...
Hyperthermophilic glycoside hydrolase family 12 endocellulase (EGPf) from the archaeon Pyrococcus furiosus catalyzes hydrolytic cleavage of β‐1,4‐glucosidic linkage in β‐glucan cellulose. A truncated EGPf (EGPfΔN30) mutant lacking proline and hydroxyl‐residue rich region at N terminus was constructed, its crystal structure resolved an atomic resolution 1.07 Å. Our results indicate that EGPf, which consists a β‐jelly roll, exhibits structural similarity with Thermotoga maritima ....
Ferulic acid and related hydroxycinnamic acids, used as antioxidants preservatives in the food, cosmetic, pharmaceutical biotechnology industries, are among most abundant phenolic compounds present plant biomass. Identification of novel that can produce ferulic safe be mass-produced, is critical for sustainability these industries. In this study, we aimed to obtain characterize a feruloyl esterase (LaFae) from Lactobacillus acidophilus. Our results demonstrated LaFae reacts with ethyl...
The hyperthermophilic endocellulase, EGPh (glycosyl hydrolase family 5) from Pyrococcus horikoshii possesses 4 cysteine residues forming 2 disulfide bonds, as identified by structural analysis. One of the bonds is located at proximal region active site in EGPh, which exhibits a distinct pattern that thermophilic endocellulase EGAc Acidothermus cellulolyticus despite similarity between two endocellulases. and suggests structure suitable for changing position bond corresponding to EGAc....
Abstract The ubiX gene of Colwellia psychrerythraea strain 34H encodes a 3-octaprenyl-4-hydroxybenzoate carboxylase (CpsUbiX, UniProtKB code: Q489U8) that is involved in the third step ubiquinone biosynthesis pathway and harbors flavin mononucleotide (FMN) as potential cofactor. Here, we report crystal structures two forms CpsUbiX: an FMN-bound wild type form FMN-unbound V47S mutant form. CpsUbiX dodecameric enzyme each monomer possesses typical Rossmann-fold structure. FMN-binding domain...