A5006021360- Enzyme Catalysis and Immobilization
- Microbial Metabolic Engineering and Bioproduction
- Enzyme Structure and Function
- Enzyme Production and Characterization
- Biofuel production and bioconversion
- Legume Nitrogen Fixing Symbiosis
- DNA and Nucleic Acid Chemistry
- Antibiotic Resistance in Bacteria
- Plant nutrient uptake and metabolism
- Advanced biosensing and bioanalysis techniques
- Pancreatic function and diabetes
- Microbial Natural Products and Biosynthesis
- RNA and protein synthesis mechanisms
- Genomics and Chromatin Dynamics
- Bacterial Genetics and Biotechnology
- Bacteriophages and microbial interactions
- Probiotics and Fermented Foods
- Analytical Chemistry and Chromatography
- Electrochemical sensors and biosensors
- Enzyme function and inhibition
- Protein Hydrolysis and Bioactive Peptides
- RNA Research and Splicing
- Antimicrobial Resistance in Staphylococcus
- biodegradable polymer synthesis and properties
- Microbial Metabolites in Food Biotechnology
Sungkyunkwan University
2016-2025
Sookmyung Women's University
2015-2023
Samsung (South Korea)
2016-2022
Samsung Medical Center
2018
Abstract Background The rapid emergence of multiple drug-resistant (MDR) bacterial pathogens and the lack a novel antibiotic pipeline pose serious threat to global healthcare. limited number established targets further restricts identification antibiotics treat life-threatening MDR infections caused by Staphylococcus aureus strains. Therefore, for developing are urgently required. In this study, we hypothesized that G-quadruplex (G4)-binding ligands can be used as their binding possibly...
A novel microbial esterase, EaEST, from a psychrophilic bacterium Exiguobacterium antarcticum B7, was identified and characterized. To our knowledge, this is the first report describing structural analysis biochemical characterization of an esterase isolated genus Exiguobacterium. Crystal structure determined at resolution 1.9 Å, showed that enzyme has canonical α/β hydrolase fold with α-helical cap domain catalytic triad consisting Ser96, Asp220, His248. Interestingly, active site EaEST...
Gram-negative bacteria are becoming resistant to almost all currently available antibiotics. Systemically designed antimicrobial peptides (AMPs) attractive agents enhance the activities of We constructed a small Pro-scanning library using amphipathic model peptides. Measurements minimum inhibitory concentration (MIC) against Escherichia coli and hemolytic showed that one Pro-hinged peptides, KL-L9P, displays highest specificity toward E. coli. Moreover, KL-L9P sensitizes be responsive most...
Biodiesel and flavor compound production using enzymatic transesterification by microbial lipases provides mild reaction conditions low energy cost compared to the chemical process. SGNH-type are very effective catalysts for due their high rate, great stability, relatively small size convenient genetic manipulations, ease of immobilization. Hence, it is highly important identify novel with catalytic efficiencies good stabilities.A promiscuous cold-adapted lipase (HaSGNH1) from...
I-motif or C4 is a four-stranded DNA structure with protonated cytosine:cytosine base pair (C+:C) found in cytosine-rich sequences. We have that oligodeoxynucleotides containing adenine and cytosine repeats form stable secondary at physiological pH magnesium ion, which similar to i-motif structure, named this 'adenine:cytosine-motif (AC-motif)'. AC-motif contains C+:C pairs intercalated putative A+:C between cytosine. By investigation of the present CDKL3 promoter (AC-motifCDKL3), one...
Cold-active acetyl xylan esterases allow for reduced bioreactor heating costs in bioenergy production. Here, we isolated and characterized a cold-active esterase (PbAcE) from the psychrophilic soil microbe Paenibacillus sp. R4. The enzyme hydrolyzes glucose penta-acetate acetate, reversibly producing xylan, it shows higher activity at 4°C than 25°C. We solved crystal structure of PbAcE 2.1-Å resolution to investigate its active site reason low-temperature activity. Structural analysis showed...
S-Formylglutathione is hydrolyzed to glutathione and formate by an S-formylglutathione hydrolase (SFGH) (3.1.2.12). This thiol esterase belongs the family also known as D. SFGHs contain highly conserved active residues of Ser-Asp-His a catalytic triad at site. Characterization investigation SFGH from Antarctic organisms molecular level needed for industrial use through protein engineering.A novel cold-active (SfSFGH) Shewanella frigidimarina, composed 279 amino acids with mass ~ 31.0 kDa,...
Abstract Considering that the prevalence of antibiotic-resistant pathogenic bacteria is largely increasing, a thorough understanding penicillin-binding proteins (PBPs) great importance and crucial significance because this enzyme family main target β-lactam-based antibiotics. In work, combining biochemical structural analysis, we present new findings provide novel insights into PBPs. Here, PBP homologue (CcEstA) from Caulobacter crescentus CB15 was characterized using native-PAGE, mass...
Ferulic acid and related hydroxycinnamic acids, used as antioxidants preservatives in the food, cosmetic, pharmaceutical biotechnology industries, are among most abundant phenolic compounds present plant biomass. Identification of novel that can produce ferulic safe be mass-produced, is critical for sustainability these industries. In this study, we aimed to obtain characterize a feruloyl esterase (LaFae) from Lactobacillus acidophilus. Our results demonstrated LaFae reacts with ethyl...
The SGNH family esterases are highly effective biocatalysts due to their strong catalytic efficiencies, great stabilities, relatively small sizes, and ease of immobilization. Here, a novel esterase (LaSGNH1) from Lactobacillus acidophilus NCFM, which has homologues in many species, was identified, characterized, immobilized. LaSGNH1 is active towards acetate- or butyrate-containing compounds, such as p-nitrophenyl acetate 1-naphthyl acetate. Enzymatic properties LaSGNH1, including thermal...
Bacterial hormone-sensitive lipases (bHSLs), which are homologous to the catalytic domains of human HSLs, have received great interest due their uses in preparation highly valuable biochemicals, such as drug intermediates or chiral building blocks. Here, a novel cold-active HSL from Halocynthiibacter arcticus (HaHSL) was examined and its enzymatic properties were investigated using several biochemical biophysical methods. Interestingly, HaHSL acted on large variety substrates including...
Molecular information about family VIII esterases, which have similarities with class C β-lactamases and penicillin-binding proteins, remains largely unknown. In this study, a novel esterase β-lactamase activity (PsEstA) from Paenibacillus sp. was characterized using several biochemical biophysical methods. PsEstA effective on broad range of substrates including tertiary butyl acetate, glyceryl tributyrate, glucose pentaacetate, olive oil, p-nitrophenyl esters. Additionally, hydrolyzed...
Pioneer transcription factors (TFs) like SOX2 are vital for stemness and cancer through enhancing gene expression within transcriptional condensates formed with coactivators, RNAs mediators on super-enhancers (SEs). Despite their importance, how these work together condensation activation remains unclear. SOX2, a pioneer TF found in SEs of pluripotent stem cells, initiates SE-mediated by binding to nucleosomes, though the mechanism isn't fully understood. To address SOX2's role SEs, we...
Carbohydrate-active enzymes (CAZymes) regulate the synthesis, degradation, and modification of poly—and oligosaccharides in all three kingdoms life. A novel carbohydrate acetylesterase from Sinorhizobium meliloti, designated SmAcE1, was identified, characterized, crystallized. This SmAcE1 is classified into esterase family 3 (CE3) based on sequence alignments with other currently known (CE) enzymes. The crystallized as a hexamer space group P212121 unit cell parameters: = 99.12 Å, b 148.88 c...
In spite of the enormous biological and clinical significance penicillin-binding protein (PBP)/β-lactamase (βL), few their many homologs (PBP)/βLs homologs) have been studied crystallographically, known functions. Herein, X-ray crystallographic study a PBP/βL homolog (Rp46) from Ruegeria pomeroyi is described. Multiple sequence alignments indicate that Rp46 has conserved serine residue within S70-X-X-K73 motif (Motif I), acting as catalytic nucleophile. Moreover, an invariant tyrosine...
This study aimed to elucidate the crystal structure and biochemically characterize carboxylesterase EaEst2, a thermotolerant biocatalyst derived from Exiguobacterium antarcticum, psychrotrophic bacterium. Sequence phylogenetic analyses showed that EaEst2 belongs Family XIII group of carboxylesterases. has broad range substrate specificities for short-chain p-nitrophenyl (pNP) esters, 1-naphthyl acetate (1-NA), butyrate (1-NB). Its optimal pH is 7.0, losing its enzymatic activity at...