A5050591399- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Angiogenesis and VEGF in Cancer
- Mesenchymal stem cell research
- Muscle Physiology and Disorders
- Bone Tissue Engineering Materials
- Cancer and Skin Lesions
- Skin Diseases and Diabetes
- Cardiac Fibrosis and Remodeling
- Drug-Induced Adverse Reactions
- Chemotherapy-related skin toxicity
- Autoimmune Bullous Skin Diseases
- Cardiac Ischemia and Reperfusion
- Drug-Induced Ocular Toxicity
- Genital Health and Disease
- Oral Health Pathology and Treatment
- Congenital heart defects research
- Pluripotent Stem Cells Research
- Cutaneous lymphoproliferative disorders research
- Virus-based gene therapy research
- Autoimmune and Inflammatory Disorders
- Nonmelanoma Skin Cancer Studies
- Dermatology and Skin Diseases
- Chronic Lymphocytic Leukemia Research
- Melanoma and MAPK Pathways
Tulane University
2018-2024
Roswell Park Comprehensive Cancer Center
2022-2024
University of Ottawa
2008-2019
EMBL Australia
2014
Monash University
2014
Australian Regenerative Medicine Institute
2014
Sapienza University of Rome
2011-2013
Istituto Pasteur
2013
Ottawa Hospital Research Institute
2013
Ottawa Hospital
2013
Cell therapy for the treatment of cardiovascular disease has been hindered by low cell engraftment, poor survival, and inadequate phenotype function. In this study, we added chitosan to a previously developed injectable collagen matrix, with aim improving its properties neovascularization. Different ratios were mixed chemically crosslinked produce hydrogels. Swell degradation assays showed that improved stability hydrogel. culture, endothelial cells formed significantly more vascular-like...
Although many regenerative cell therapies are being developed to replace or regenerate ischaemic muscle, the lack of vasculature and poor persistence therapeutic cells represent major limiting factors successful tissue restoration.In response ischaemia, stromal cellderived factor-1 (SDF-1) is up-regulated by affected stimulate stem cell-mediated responses.Therefore, we encapsulated SDF-1 into alginate microspheres further incorporated these an injectable collagen-based matrix in order...
Background— Blood-derived circulatory angiogenic cells (CACs) and resident cardiac stem (CSCs) have both been shown to improve function after myocardial infarction. The superiority of either cell type has long an area speculation with no definitive head-to-head trial. In this study, we compared the effect human CACs CSCs, alone or in combination, on immunodeficient mouse model Methods Results— CSCs were cultured from left atrial appendages blood samples obtained patients undergoing...
Wolf isotopic response refers to a new skin disorder manifesting in the area of previous lesion that has healed. A variety disease processes and dermatoses have been described evoke an response. We report man with metastatic hepatocellular carcinoma who received palliative radiation later experienced pembrolizumab-induced lichenoid dermatitis at same site radiation, representing cutaneous immune-related adverse event displaying
We sought to identify an essential component of the TEAD4/VGLL4 transcription factor complex that controls vascular endothelial growth A (VEGFA) expression in muscle. yeast 2-hybrid screen was used clone a novel TEAD4 from human heart cDNA library. identified interferon response 2 binding protein (IRF2BP2) and confirmed its presence vivo by coimmunoprecipitation mammalian assays. Coexpression IRF2BP2 with or TEAD1 alone potently activated, whereas knockdown reduced, VEGFA C(2)C(12) muscle...
Circulating progenitor cells home to and engraft sites of ischemia, mediated in part by the adhesion molecule L-selectin; however, accumulation tissues such as heart is low. In this study, an acellular collagen-based matrix containing sialyl Lewis(X) (sLe(X)), which binds L-selectin, was developed order enhance endogenous cell therapeutic response. Its effect on angiogenesis were assessed vitro using a hindlimb ischemia model with rats. culture, sLe(X)-collagen recruited more...
Methylglyoxal (MG) accumulates in diabetes and impairs neovascularization. This study assessed whether overexpressing the MG-metabolizing enzyme glyoxalase-1 (GLO1) only bone marrow cells (BMCs) could restore neovascularization ischaemic tissue of streptozotocin-induced diabetic mice. After 24 h hyperglycaemic hypoxic culture, BMCs from GLO1 wild-type (WT) mice were compared for migratory potential, viability, mRNA expression anti-apoptotic genes (Bcl-2 Bcl-XL). In vivo, enhanced green...
Biomaterial-guided regeneration represents a novel approach for the treatment of myopathies. Revascularisation and intramuscular extracellular matrix are important factors in stimulating myogenesis regenerating muscle damaged by ischaemia. In this study, we used an injectable collagen matrix, enhanced with sialyl LewisX (sLeX), to guide skeletal differentiation regeneration. The elastic properties sLeX-collagen matrices were similar those muscle, culture pluripotent mESCs on promoted their...
Cell therapy is expected to restore perfusion and improve function in the ischemic/infarcted myocardium; however, biological mechanisms local effects of transplanted cells remain unclear. To assess cell fate vivo, hexadecyl-4-[(18)F]fluorobenzoate ((18)F-HFB) labeling was evaluated for tracking human circulating progenitor (CPCs) with positron emission tomography (PET) compared commonly used 2-[(18)F]fluoro-2-deoxy-d-glucose ((18)F-FDG) method a rat myocardial infarction model. CPCs were...
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Potential benefits of co-culturing monocytes (MC) with vascular smooth muscle cells have been reported on for tissue engineering applications a degradable, polar, hydrophobic, and ionic polyurethane (D-PHI). Since the interaction MC endothelial (EC) within blood vessel endothelium is also process wound repair it was interest to investigate their function when cultured synthetic D-PHI materials, prior considering materials' use in engineering. The co-culture (MC/EC) vitro studies were carried...
Abstract Hydroxychloroquine (HCQ) is an immunomodulator used in dermatology and rheumatology. Side effects may be observed on routine monitoring studies before they become clinically apparent. The goal of this retrospective chart review was to assess laboratory abnormalities dermatologic rheumatologic patients taking HCQ. Medical records prescribed HCQ were retrospectively reviewed. Demographics, reported side effects, parameters baseline follow-up complete blood count (CBC) comprehensive...