A5101439431- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Advanced Electron Microscopy Techniques and Applications
- Protist diversity and phylogeny
- Fungal and yeast genetics research
- Hepatitis Viruses Studies and Epidemiology
- Microfluidic and Bio-sensing Technologies
- Microbial Community Ecology and Physiology
- Nanowire Synthesis and Applications
- Assisted Reproductive Technology and Twin Pregnancy
- Force Microscopy Techniques and Applications
- Plant Virus Research Studies
- Yeasts and Rust Fungi Studies
- Electron and X-Ray Spectroscopy Techniques
- Electrospun Nanofibers in Biomedical Applications
- Genomics and Phylogenetic Studies
- RNA and protein synthesis mechanisms
- Congenital Anomalies and Fetal Surgery
- Fungal Biology and Applications
- Advanced Fluorescence Microscopy Techniques
- Maternal Mental Health During Pregnancy and Postpartum
- Single-cell and spatial transcriptomics
Carilion Clinic
2012-2024
Virginia Tech
2012-2024
Wake Forest University
2018-2024
Virginia–Maryland College of Veterinary Medicine
2012-2017
Roanoke College
2015
National Institutes of Health
2008-2014
National Institute of Allergy and Infectious Diseases
2008-2014
University of Ottawa
2011
Monterey Bay Aquarium Research Institute
2009-2010
SRI International
2010
ABSTRACT Group A rotavirus classification is currently based on the molecular properties of two outer layer proteins, VP7 and VP4, middle protein, VP6. As reassortment all 11 gene segments plays a key role in generating diversity nature, system that desirable for determining which genes influence host range restriction, replication, virulence, as well studying epidemiology evolution. Toward establishing such system, sequences encoding VP1 to VP3, VP6, NSP1 NSP5 were determined human animal...
Picoeukaryotes are a taxonomically diverse group of organisms less than 2 micrometers in diameter. Photosynthetic marine picoeukaryotes the genus Micromonas thrive ecosystems ranging from tropical to polar and could serve as sentinel for biogeochemical fluxes modern oceans during climate change. These broadly distributed primary producers belong an anciently diverged sister clade land plants. Although isolates have high 18S ribosomal RNA gene identity, we found that genomes two shared only...
Group A human rotaviruses (RVs) are a major cause of severe gastroenteritis in infants and young children. Yet, aside from the genes encoding serotype antigens (VP7; G-type VP4; P-type), little is known about genetic make-up emerging endemic RV strains. To gain insight into diversity evolution RVs circulating at single location over period time, we sequenced eleven-segmented, double-stranded RNA genomes fifty-one G3P[8] strains collected 1974 to 1991 Children's Hospital National Medical...
Morphometric shape analysis and ultrastructural data are provided for six genodemes of the Sellaphora pupula species complex that have been studied during last 20 years from Blackford Pond, Edinburgh, UK. The demes previously shown to be separated by prezygotic reproductive barriers: cells different do not pair, though they may show residual interactions when sexualized. A new morphometric method, contour segment analysis, which was recently developed diatoms in relation automated...
Abstract Group A rotaviruses (RVs) infect the young of numerous animal species and cause acute gastroenteritis. Cultivation human RVs in cells requires proteolytic activation viral attachment protein using trypsin. Continuous cell lines, such as rhesus monkey kidney cells, well primary are routinely used for growth characterization RVs. Isolation cultivation from clinical fecal specimens is difficult adaptation to vitro multiple rounds passage cells. Following growth, RV stocks can be...
ABSTRACT Group A human rotaviruses (HRVs) are the major cause of severe viral gastroenteritis in infants and young children. To gain insight into level genetic variation among HRVs, we determined genome sequences for 10 strains belonging to different VP7 serotypes (G types). The HRVs chosen this study, D, DS-1, P, ST3, IAL28, Se584, 69M, WI61, A64, L26, were isolated from infected persons adapted cell culture use as serotype references. Our sequencing results revealed that most individual...
The prasinophyte order Mamiellales contains several widespread marine picophytoplankton (≤ 2 μm diameter) taxa, including Micromonas and Ostreococcus. Complete genome sequences are available for two isolates, CCMP1545 RCC299. We performed in silico analyses of nitrogen transporters related assimilation genes RCC299 compared these with other green lineage organisms as well Chromalveolata, fungi, bacteria, archaea. Phylogenetic reconstructions ammonium transporter (AMT) revealed divergent...
The innermost VP2 core shell of the triple-layered, icosahedral rotavirus particle surrounds viral genome and RNA processing enzymes, including RNA-dependent polymerase (VP1). In addition to anchoring VP1 within core, is also an essential cofactor that triggers initiate double-stranded (dsRNA) synthesis using packaged plus-strand templates. requirement effectively couples packaging with replication ensures makes dsRNA only assembling previrion particle. However, mechanism by which protein...
Group A rotaviruses (RVs) remain a leading cause of childhood gastroenteritis worldwide. Although the G/P types locally circulating RVs can vary from year to and differ depending upon geographical location, those with G1P[8], G2P[4], G3P[8], G4P[8], G9P[8], G12P[8] specificities typically dominate. Little is known about evolution diversity G2P[4] possible role that widespread vaccine use has had on their increased frequency detection. To address these issues, we analyzed 12 RV isolates...
Rotaviruses perform the remarkable tasks of transcribing and replicating 11 distinct double-stranded RNA genome segments within confines a subviral particle. Multiple viral polymerases are tethered to interior particle, each dedicated solitary segment but acting in synchrony synthesize RNA. Although rotavirus polymerase specifically recognizes templates absence other proteins, its enzymatic activity is contingent upon interaction with capsid. This intraparticle strategy synthesis helps...
ABSTRACT Group A rotaviruses (RVs) are 11-segmented, double-stranded RNA viruses and primary causes of gastroenteritis in young children. Despite their medical relevance, the genetic diversity modern human RVs is poorly understood, impact vaccine use on circulating strains remains unknown. In this study, we report complete genome sequence analysis 58 isolated from children with severe diarrhea and/or vomiting at Vanderbilt University Medical Center (VUMC) Nashville, TN, during years spanning...
We present a novel microfluidic platform to examine biological assemblies at high-resolution. have engineered functionalized chamber that serves as "nanoscale biosphere" capture and maintain rotavirus double-layered particles (DLPs) in liquid environment. The can be inserted into the column of transmission electron microscope while being completely isolated from vacuum system. This configuration allowed us determine structure complexes nanometer-resolution within self-contained vessel....
We present the first high-resolution movies of active viral assemblies in liquid using <italic>in situ</italic> TEM.
ABSTRACT Rotaviruses (RVs) are nonenveloped, 11-segmented, double-stranded RNA viruses that major pathogens associated with acute gastroenteritis. Group A, B, and C RVs have been isolated from humans; however, intergroup gene reassortment does not occur for reasons remain unclear. This restriction might reflect the failure of viral RNA-dependent polymerase (RdRp; VP1) to recognize replicate a different group. To address this possibility, we contrasted sequences, structures, functions RdRps...