Antibiotic resistance is a problem of deep scientific concern both in hospital and community settings. Rapid detection clinical laboratories essential for the judicious recognition antimicrobial resistant organisms. Production extended-spectrum β-lactamases (ESBLs) significant resistance-mechanism that impedes treatment infections caused by Enterobacteriaceae serious threat to currently available antibiotic armory. ESBLs are classified into several groups according their amino acid sequence...

Biosurfactants derived from different microbes are an alternative to chemical surfactants, which have broad applications in food, oil, biodegradation, cosmetic, agriculture, pesticide and medicine/pharmaceutical industries. This is due their environmentally friendly, biocompatible, biodegradable, effectiveness work under various environmental conditions non-toxic nature. Lactic acid bacteria (LAB)-derived glycolipid biosurfactants can play a major role preventing bacterial attachment,...

Cefotaxime (CTX) is a third-generation cephalosporin antibiotic with broad-spectrum activity against Gram negative and positive bacteria. However, like other antibiotics, its efficacy declining due to the increased prevalence of multidrug-resistant (MDR) pathogens. Recent advances in nanotechnology have been projected as practical approach combat MDR microorganisms. Therefore, current study, gold nanoparticles (AuNPs) were prepared using cefotaxime sodium, which acted reducing capping agent,...

In the present study, objective was to attain a localized lung delivery of an anti-tubercular fluoroquinolone, moxifloxacin (MXF), targeting alveolar macrophages through non-invasive pulmonary route using inhalable microspheres as dry powder inhaler approach. MXF-loaded poly (lactic-co-glycolic acid) (PLGA) (MXF-PLGA-MSs) were fabricated by solvent evaporation technique and optimized central composite statistical design. The morphology particle size, well flowability microspheres,...

The purpose of this study was to fabricate biostable inorganic silver nanoparticles (AgNPs) using fresh peel (aqueous) extract Benincasa hispida. A fast, robust, and eco-friendly approach used for the synthesis AgNPs, where bioactive components B. hispida acted as reducing stabilizing agents. Synthesized AgNPs were characterized a UV–Vis spectrophotometer, Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), electron microscopy. synthesized exhibited maximum...

Polymeric nanosponges as potential carriers for successful combination therapy of poorly soluble drugs (paracetamol, aceclofenac, caffeine).

Alzheimer’s disease (AD) is a progressive neurological disorder that affects 50 million people. Despite this, only two classes of medication have been approved by the FDA. Therefore, we planned to develop therapeutics multitarget approach. We explored library 2029 natural product-like compounds for their multi-targeting potential against AD inhibiting AChE, BChE (cholinergic pathway) MAO-A, and MOA-B (oxidative stress through in silico high-throughput screening molecular dynamics simulation....

The remarkable rise of antibiotic resistance among pathogenic bacteria poses a significant threat to human health. Nanoparticles (NPs) have recently emerged as novel strategies for conquering fatal bacterial diseases. Furthermore, antibiotic-functionalized metallic NPs represent viable nano-platform combating resistance. In this study, we present the use vancomycin-functionalized gold nanoparticles (V-GNPs) battle strains. A facile one-pot method was adopted synthesize vancomycin-loaded GNPs...

New antibiotics are seen as 'drugs of last resort' against virulent bacteria. However, development resistance towards new with time is a universal fact. Delafloxacin (DFX) fluoroquinolone antibiotic that differs from existing fluoroquinolones by the lack protonatable substituent, which gives molecule weakly acidic nature, affording it higher antibacterial activity under an environment. Furthermore, antibiotic-functionalized metallic nanoparticles have been recently emerged feasible platform...

Enterococci are troublesome nosocomial, opportunistic Gram-positive cocci bacteria showing enhanced resistance to many commonly used antibiotics. This study aims investigate the prevalence and genetic basis of antibiotic macrolides, lincosamides, streptogramins (MLS) in Enterococci, as well correlation between MLS biocide resistance. From 913 clinical isolates collected from King Khalid Hospital, Hail, Saudi Arabia, 131 were identified spp. The susceptibility enterococcal several antibiotics...

Numerous clinical and epidemiological studies have provided direct evidence to strengthen the link between type 2 diabetes (T2D) Alzheimer's disease (AD). The possibility that T2D patients might be at increased risk in developing AD has serious societal implications. Sodium glucose co-transporter (SGLT2) is one of best targets treatment diabetes, whereas acetylcholinesterase (AChE) long been regarded as a therapeutic target for AD. This study explores molecular interactions AChE SGLT2 with...

Acetylcholinesterase (AChE) is a primary target for Alzheimer's therapy while recently sodium glucose cotransporter 2 (SGLT2) has gained importance as potential Type Diabetes Mellitus (T2DM) therapy. The present study emphasizes the molecular interactions between new Food and Drug Administration (FDA) approved antidiabetic drug 'Invokana' (chemically known Canagliflozin) with AChE SGLT2 to establish link treatment of T2DM Disease (AD). Docking was performed using 'Autodock4.2'. Both...

Multidrug-resistance due to "β lactamases having the expanded spectrum" (ESBLs) in members of Enterobacteriaceae is a matter continued clinical concern. CTX-M among most common ESBLs family. In present study, nanoformulation cefotaxime was prepared using gold nanoparticles combat drug-resistance ESBL producing strains. Here, two CTX-M-15 positive resistant bacterial strains (i.e., one Escherichia coli and Klebsiella pneumoniae strain) were used for testing efficacy "cefotaxime loaded...

Alveolar macrophages are the first line of defense against intruding pathogens and play a critical role in cancer immunology. The Toll-like receptor (TLR) family mediates an important recognizing mounting immune response microbes. TLR-9 is member intracellular TLR family, which recognizes unmethylated CG motifs from prokaryotic genome. Upon its activation, triggers downstream MyD-88-dependent transcriptional activation NF-κB, subsequently results abundant inflammatory cytokines expression...

Calcium homeostasis modulator 1 (CALHM1) is a protein responsible for causing Alzheimer's disease. In the absence of an experimentally designed molecule, homology modelling was performed. Through modelling, different CALHM1 models were generated and validated through Rampage. To carry out further in silico studies, molecular docking dynamics simulation experiments, various flavonoids alkaloids from Bauhinia variegata utilised as inhibitors to target (CALHM1). The sequence retrieved UniProt...

Among the various types of cancer, lung cancer accounts for highest number fatalities across globe. A combination different chemotherapeutics is regarded as an effective strategy clinical management cancers. Ganetespib (GAN) a well-established hsp90 inhibitor with enhanced pharmacological properties in comparison its first-generation counterparts. Previous preclinical studies have shown that GAN exerts significant effects against cells; however, therapeutic non-small cell (NSCLC) A549 cells,...

At the molecular level, several developmental signaling pathways, such as Wnt/β-catenin, have been associated with initiation and subsequent progression of prostate carcinomas. The present report elucidated anti-cancerous attributes an anthraquinone, aloe-emodin (AE), against androgen-independent human cancer DU145 cells. cytotoxicity profiling AE showed that it exerted significant cytotoxic effects increased lactose dehydrogenase levels in cells (

Muscular dystrophies (MDs) are an array of inherited disorders characterized by progressive muscle weakness and degeneration. This review explores the genetic foundation, pathophysiological mechanisms, current treatments, therapeutic potential medicinal plants for MDs. Although gene therapy, exon-skipping, stem cell therapies substantial advancements, they encounter obstacles regarding immune responses, long-term efficacy, delivery efficiency. A promising complementary approach to...

Sulforaphane is a main bioactive component in several edible cruciferous vegetables. It acquires benefits to health addition its considered antibacterial and antivirulence activities. Herein, we aimed at evaluating the activity of sulforaphane against worldwide clinically important enteric pathogen Salmonella enterica serovar Typhimurium. The influence on bacterial adhesion, invasion, biofilm formation, intracellular replication was assayed. Additionally, effect type III secretion system...