A5075277277- Antimicrobial Peptides and Activities
- Biochemical and Structural Characterization
- Transgenic Plants and Applications
- Antibiotic Resistance in Bacteria
- Plant-Microbe Interactions and Immunity
- Toxin Mechanisms and Immunotoxins
- Bacterial biofilms and quorum sensing
- Plant Pathogenic Bacteria Studies
- Probiotics and Fermented Foods
- Venomous Animal Envenomation and Studies
- Chemical Synthesis and Analysis
- ATP Synthase and ATPases Research
- Machine Learning in Bioinformatics
- Plant Disease Resistance and Genetics
- Protein Hydrolysis and Bioactive Peptides
- Plant pathogens and resistance mechanisms
- Aquaculture disease management and microbiota
- Essential Oils and Antimicrobial Activity
- Supramolecular Self-Assembly in Materials
- Bacteriophages and microbial interactions
- Invertebrate Immune Response Mechanisms
- Phytoplasmas and Hemiptera pathogens
- Cancer Research and Treatments
- Marine Invertebrate Physiology and Ecology
- Agricultural pest management studies
Universidade Católica de Brasília
2015-2025
Universidade Católica Dom Bosco
2015-2025
Biotech (Brazil)
2019
Bacterial biofilms and associated infections represent one of the biggest challenges in clinic, as an alternative to counter bacterial infections, antimicrobial peptides have attracted great attention past decade. Here, ten short cationic were generated through a sliding-window strategy on basis 19-amino acid residue peptide, derived from Pyrobaculum aerophilum ribosomal protein. PaDBS1R6F10 exhibited anti-infective potential it decreased burden murine Pseudomonas aeruginosa cutaneous by...
Antimicrobial peptides (AMPs) are effective antibiotic agents commonly found in plants, animals, and microorganisms, they have been suggested as the future of antimicrobial chemotherapies. It is vital to understand molecular details that define mechanism action resistance AMPs for a rational planning next generation also shed some light on complex AMP action. Here, Escherichia coli ATCC 8739 magainin I was evaluated cytosolic subproteome. Magainin-resistant strains were selected after 10...
Stingrays commonly cause human envenoming related accidents in populations of the sea, near rivers and lakes. Transcriptomic profiles have been used to elucidate components animal venom, since they are capable providing molecular information on biology could biomedical applications. In this study, we elucidated transcriptomic profile venom glands from two different freshwater stingray species that endemic Paraná-Paraguay basin Brazil, Potamotrygon amandae falkneri. Using RNA-Seq, identified...
Computer-aided screening of antimicrobial peptides (AMPs) is a promising approach for discovering novel therapies against multidrug-resistant bacterial infections. Here, we functionally and structurally characterized an Escherichia coli-derived AMP (EcDBS1R5) previously designed through pattern identification [α-helical set (KK[ILV](3)[AILV])], followed by sequence optimization. EcDBS1R5 inhibited the growth Gram-negative Gram-positive, susceptible resistant strains at low doses (2–32 μM),...
Diverse peptides have been evaluated for their activity against pathogenic microorganisms. Here, five mastoparan variants were designed based on mastoparan-L, among which two (R1 and R4) selected in-depth analysis. Mastoparan-L (parent/control), R1, R4 inhibited susceptible/resistant bacteria at concentrations ranging from 2 to 32 μM, whereas only R1 eradicated Pseudomonas aeruginosa biofilms 16 μM. Moreover, the toxic effects of mastoparan-L toward mammalian cells drastically reduced in...
Zantedeschia aethiopica is an evergreen perennial plant cultivated worldwide and commonly used for ornamental medicinal purposes including the treatment of bacterial infections. However, current understanding molecular physiological mechanisms in this limited, comparison to other non-model plants. In order improve biology botanical species, RNA-Seq technology was transcriptome assembly characterization. Following Z. spathe tissue RNA extraction, high-throughput sequencing performed with aim...
In recent years the antimicrobial peptides (AMPs) have been prospected and designed as new alternatives to conventional antibiotics. Indeed, AMPs presented great potential toward pathogenic bacterial strains by means of complex mechanisms action. However, reports increasingly emerged regarding which bacteria resist AMP administration. this context, we performed a comparative proteomic study using total lysate magainin I-susceptible -resistant E. coli strains. After nanoUPLC-MSE analyses...
An unusual N-capping asparagine-lysine-proline (5NKP7) motif yields a coil/N-cap/α-helix multifunctional scaffold in computer-made peptide selective for anionic surfaces and with anticancer, antibacterial, antibiofilm, anti-infective ( vivo ), immunomodulatory potential.
In order to study how acidic pro-peptides inhibit the antimicrobial activity of peptides, we introduce a simple model system, consisting 19 amino-acid long peptide, and an N-terminally attached, 10 pro-peptide. The peptide is fragment crotalicidin member cathelidin family, from rattlesnake venom. pro-peptide deca (glutamic acid). Attachment only leads moderately large reduction in binding to- induced leakage liposomes, while completely inhibited by attaching Attaching induces conformational...