A5031773119- Zeolite Catalysis and Synthesis
- Bacterial Genetics and Biotechnology
- Catalysis for Biomass Conversion
- RNA and protein synthesis mechanisms
- Enzyme Production and Characterization
- Catalysis and Hydrodesulfurization Studies
- CRISPR and Genetic Engineering
- Genomics and Phylogenetic Studies
- Liver Disease Diagnosis and Treatment
- Bacteriophages and microbial interactions
- Liver physiology and pathology
- Metalloenzymes and iron-sulfur proteins
- Electron Spin Resonance Studies
- Various Chemistry Research Topics
- Viral Infectious Diseases and Gene Expression in Insects
- Sulfur-Based Synthesis Techniques
- Ammonia Synthesis and Nitrogen Reduction
- Molecular spectroscopy and chirality
- Hearing, Cochlea, Tinnitus, Genetics
- Microbial Metabolic Engineering and Bioproduction
- Neurological Complications and Syndromes
- Electrocatalysts for Energy Conversion
- Protein Structure and Dynamics
- Adenosine and Purinergic Signaling
- Catalytic Alkyne Reactions
BASF (Germany)
2025
Chalmers University of Technology
2021-2024
Newcastle University
2016-2018
DSM (Netherlands)
2016-2018
University of Bologna
2017
Ludwig-Maximilians-Universität München
2013
Luther University
2011
Martin Luther University Halle-Wittenberg
2011
Leipzig University
2006-2008
University of Vienna
1987
Standardized and well-characterized genetic building blocks are a prerequisite for the convenient reproducible assembly of novel modules devices. While numerous standardized parts exist Escherichia coli, such tools still missing Gram-positive model organism Bacillus subtilis. The goal this study was to develop thoroughly evaluate toolbox.We developed five BioBrick-compatible integrative B. subtilis vectors by deleting unnecessary removing forbidden restriction sites allow cloning in BioBrick...
Complex cellular networks regulate regeneration, detoxification and differentiation of hepatocytes. By combining experimental data with mathematical modelling, systems biology holds great promises to elucidate the key regulatory mechanisms involved predict targets for efficient intervention. For generation high-quality quantitative suitable modelling a standardised in vitro system is essential. Therefore authors developed standard operating procedures preparation cultivation primary mouse To...
A fixed gene copy number is important for the in silico construction of engineered synthetic networks. However, integrated genes depends on their genomic location. This dosage effect rarely addressed biology. Two studies Escherichia coli presented conflicting data impact dosage. Here, we investigate how genome location and orientation influences expression Bacillus subtilis. An difference with E. that used an unbiased integration approach mediated by random transposon insertion. We found...
Abstract A visible‐light driven Suzuki cross‐coupling reaction was performed with colored and bench‐stable arylazosulfones in the presence of Ph 3 PAuCl (5 mol %) as catalyst. The absence a photocatalyst, along use commercially available easy‐to‐handle arylboronic acids underline novelty synthetic usefulness protocol. mechanism involving generation an aryl radical key intermediate has been proposed on basis experimental investigations.
Increasing protein expression levels is a key step in the commercial production of enzymes. Predicting promoter activity and translation initiation efficiency based solely on consensus sequences have so far met with mixed results. Here, we addressed this challenge using "brute-force" approach by designing synthesizing large combinatorial library comprising ∼12 000 unique synthetic modules (SEMs) for Bacillus subtilis. Using GFP fluorescence as reporter gene expression, obtained dynamic range...
Abstract ATP acts as a growth factor well toxic agent by stimulating P2 receptors. The receptor‐activated signaling cascades mediating cellular and cell survival after injury are only incompletely understood. Therefore, the aim of present study was to identify role phosphoinositide 3 kinase (PI3‐K/Akt) mitogen‐activated protein kinase/extracellular signal regulated (MAPK/ERK) pathways in P2Y receptor‐mediated astrogliosis traumatic microinfusion ADPβS (P2Y 1,12,13 receptor agonist) into rat...
Proteins face an obstacle race on their way to successful folding. Chaperones facilitate the proper folding of proteins by ensuring they remain correct path toward final tertiary structure. In bacilli, PrsA chaperone is essential for and stabilization within cell wall. Overexpression has been shown improve secretion many biotechnologically relevant secreted enzymes. This resulted in a double benefit: firstly, it promotes efficient release properly folded enzymes from wall, second, reduces...
Abstract 2,4‐dimethylfuran has a rare disubstitution pattern in the five‐membered heterocyclic furan ring that is highly interesting chemically but challenging to access synthetically. We present heterogeneously catalysed route synthesise from commonly available 2,5‐dimethylfuran using zeolite packed‐bed flow reactor. As supported by DFT calculations, reaction occurs inside channels, where acid sites catalyse proton transfer followed migration of methyl group. The zeotype Ga‐silicate (MFI...
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTInfrared Spectrum of Cyclobutene. A CorrectionJohn D. Roberts and C. W. SauerCite this: J. Am. Chem. Soc. 1952, 74, 12, 3192–3193Publication Date (Print):June 1, 1952Publication History Published online1 May 2002Published inissue 1 June 1952https://doi.org/10.1021/ja01132a528RIGHTS & PERMISSIONSArticle Views67Altmetric-Citations4LEARN ABOUT THESE METRICSArticle Views are the COUNTER-compliant sum full text article downloads since November 2008...
On-line composition analysis of complex hydrocarbon mixtures is highly desirable to determine the process streams and study chemical reactions in heterogeneous catalysis. Here, we show how combination time-resolved Fourier transform infrared spectroscopy ion–molecule-reaction mass spectrometry (IMR-MS) can be used for compositional processed plant biomass streams. The method based on biomass-derived model compound 2,5-dimethylfuran its potential catalytic conversion valuable green aromatics,...
Abstract The valorization of biomass-derived molecules into commodity chemicals is important for the transition to renewable feedstocks. model platform molecule 2,5-dimethylfuran (2,5-dmf) can be converted value-added aromatics such as benzene, toluene, and xylenes (BTX) over zeolite catalysts. To explore role acid site(s) in BTX selectivity, gallium has been isomorphously substituted framework, resulting a Ga-silicate. Compared ZSM-5 counterpart, this modification shows enhanced benzene...
The valorisation of 2,5-dimethylfuran over zeolites under catalytic fast pyrolysis conditions was analysed by on-line FTIR-MS. BTX and olefins correlate decrease with time on stream, whereas the isomerisation increases.