A5020990186- Renal Transplantation Outcomes and Treatments
- Organ Transplantation Techniques and Outcomes
- Immune Response and Inflammation
- T-cell and B-cell Immunology
- Polyomavirus and related diseases
- Cytokine Signaling Pathways and Interactions
- Renal Diseases and Glomerulopathies
- Atherosclerosis and Cardiovascular Diseases
- Organ Donation and Transplantation
- Renal and Vascular Pathologies
- Neurological Complications and Syndromes
- Transplantation: Methods and Outcomes
- Acute Kidney Injury Research
- Hematopoietic Stem Cell Transplantation
- COVID-19 and healthcare impacts
- Organ and Tissue Transplantation Research
- Immune Cell Function and Interaction
- Amyloidosis: Diagnosis, Treatment, Outcomes
- Liver Disease and Transplantation
- Full-Duplex Wireless Communications
- Multiple Myeloma Research and Treatments
- Hepatitis C virus research
- Immune responses and vaccinations
- Pediatric Urology and Nephrology Studies
- Liver physiology and pathology
Kaiser Permanente
2021-2024
Kaiser Permanente San Francisco Medical Center
2020
Swedish Medical Center
2012
Massachusetts General Hospital
2004-2011
Harvard University
2000-2011
New York Medical College
2009
Centro Hospitalar de Vila Nova de Gaia
2009
Vanderbilt University Medical Center
2009
Iran University of Medical Sciences
2009
The Ohio State University
2009
We noted previously that ischemic acute tubular necrosis (ATN) induces local expression of MHC products in renal epithelium. The present investigations were conducted to establish the role IFN-gamma regulation antigen ATN and explore changes cytokine growth factor induced by injury. produced unilateral mice clamping left pedicle. class I II steady state mRNA induction was assessed northern blot analysis, product quantified extent binding radiolabeled monoclonals tissue homogenates. levels...
Expanded criteria donors (ECDs) and donation after cardiac death (DCD) provide more kidneys in the donor pool. However, financial impact long-term benefits of these have been questioned. From 1998 to 2005, we performed 271 deceased kidney transplants into adult recipients. There were 163 (60.1%) SCDs, 44 (16.2%) ECDs, 53 (19.6%) DCDs 11 (4.1%) ECD/DCDs. The mean follow-up was 50 months. ECD DCD had a significantly higher incidence delayed graft function, longer time reach serum creatinine...
Transcription factor interferon regulatory factor-1 (IRF-1) is implicated in regulating class I MHC expression vitro. We investigated the vivo relationship between IRF-1 and kidney other nonlymphoid organs, assessing mice with disrupted genes (IRF-1 KO) compared intact (WT). In kidneys of KO mice, basal was decreased, particularly on arterial endothelium, but II unchanged. The induction both by injected rIFN-gamma reduced KOs, WT mice. Similarly, stimuli that induce endogenous IFN-gamma...
We compared the expression of MHC class I and II products in tissues IFN-gamma knockout (GKO) vs normal (wild-type) BALB/c mice. studied basal state, after local tissue injury, stimuli that induce systemic (allogeneic cells, oxazolone skin painting, or LPS), rIFN-gamma. Basal interstitial cells was not reduced GKO However, mice had less kidney, liver, heart, arterial endothelium than wild-type Local renal ischemic injury increased kidney tubules both mice, but induction wild-type. Potent...
Renal ischemic injury evokes an inflammatory response with increased cytokine and major histocompatibility complex (MHC) expression a mild interstitial infiltrate. This "injury response" could contribute to the tendency of ischemically injured renal transplants reject. The studies presented here evaluated ability recombinant human insulin-like growth factor-1 (rhlGF-1) given after prevent inflammation. left pedicle CBA BALB/c mice was clamped for 60 min, rhlGF-1 (25, 50, 100 micrograms)...
We examined the expression of MHC class I and II in arterial endothelium interferon-γ (IFN-γ, GKO) IFN-γ-R(IFN-γ-R, GRKO) gene knockout mice comparison with intact IFN-γ IFN-γ-R genes, BALB/c 129Sv/J wild-type, respectively. The GKO GRKO were produced by targeting. was assessed mAb binding to frozen tissue (kidney, spleen, heart, liver) sections immunoperoxidase staining basal state after various stimuli: allogeneic cells, oxazolone skin sensitization, LPS, rIFN-γ. As controls, we also two...
Hosts undergoing allograft rejection show increased MHC expression locally in the graft and systemically normal host organs, mediated principally by IFN-γ. The transcription factor IRF-1 has been implicated regulation of IFNs vitro as well production some cytokines. We investigated role vivo systemic hosts allogeneic tumors comparing induction mice with genes (wild type or WT mice) disrupted (IRF-1 knockout KO mice). assessed product immunohistology radiolabeled antibody binding to tissue...
A 56-year-old woman was admitted to the hospital because of renal failure. Ten years earlier, heart–lung transplantation had been performed primary pulmonary hypertension; her immunosuppressive regimen consisted cyclosporine, prednisone, and azathioprine. Her medical history included glomerulonephritis at age 19 years, which resolved, recurrent urinary tract infections. Renal function deteriorating slowly since transplantation, proteinuria developed. diagnostic procedure performed.
Background. Belatacept, a selective T-cell costimulation blocker, was associated with improved survival and renal function but also risk of posttransplant lymphoproliferative disorder (PTLD) in adult kidney transplant recipients phase 3 trials. This registry examined long-term safety Epstein–Barr virus (EBV)–seropositive treated belatacept. Methods. US-based, prospective, voluntary, multicenter (Evaluating Nulojix Long-Term Safety Transplant [ENLiST]) included EBV-seropositive kidney-only de...
We studied major histocompatibility complex (MHC) and cytokine mRNA induction after renal injury in the absence of interferon-γ (IFN-γ) using IFN-γ gene knockout (GKO) mice. The left pedicle normal (wild-type) GKO BALB/c mice was clamped for 60 minutes; MHC expression were monitored injured kidney compared to contralateral control kidney. After a single episode ischemic injury, class I II, interleukin-2, interleukin-10, granulocyte-macrophage colony-stimulating factor, tumor necrosis...
Background. Tissue injury induces MHC class II expression, which could be important in the recognition of that tissue as an allograft. The transcriptional activator (CIITA) is major regulator basal and induced expression essential for antigen presentation. role CIITA induction by unknown. In this study, we examined course acute ischemic or toxic renal mice, including interferon (IFN)-γ transcription factor, regulatory factor (IRF)-1. Methods. Kidneys were injured ischemia gentamicin toxicity...
The class II transactivator (CIITA) is a protein that induces the transcription of MHC genes. We studied expression CIITA in vivo, comparing steady state levels and mRNA various mouse tissues. Many tissues normal mice contained for CIITA, correlating with mRNA. basal disrupted IFN-γ genes (GKO mice) was similar to wild-type mice. Injection rIFN-γ strongly induced mRNA: increased at 2 hr declined baseline by 48 hr, whereas 24 returned 7 days. Proinflammatory stimuli induce production...