Increasing epidemic of type 2 diabetes mellitus (T2DM) and its comorbidities makes it urgent to understand the pathogenesis regulatory mechanism. However, little is known about role lncRNAs in diabetes. Here, we constructed a T2DM-related competitive endogenous RNA (ceRNA) network (DMCN) explore biological function during development mellitus. This contained 351 nodes including 98 mRNAs, 86 microRNAs 167 lncRNAs. Functional analysis showed that mRNAs DMCN were annotated into some...

Macrophages are principal immune cells with a high plasticity in the human body that can differentiate under different conditions tumor microenvironment to adopt two polarized phenotypes opposite functions. Therefore, converting macrophages from immunosuppressive phenotype (M2) inflammatory (M1) is considered promising therapeutic strategy for cancer. However, molecular mechanisms underlying this conversion process have not yet been completely elucidated. In recent years, microRNAs (miRNAs...

Abstract Background Various angiogenic regulators are involved in angiogenesis cascade. Transcription factor Ets-1 plays important role angiogenesis, remodeling of extracellular matrix, and tumor metastasis. target genes various stages new blood vessel formation include angiopoietin, matrix metalloproteinases (MMPs) the protease inhibitor maspin. Methods We used immunohistochemistry (IHC) to detect expression Ets-1, angiopoietin-2 (Ang-2) maspin ovarian analyzed relationship between these...

Dual primary breast cancer (BC) and ovarian (OC) represent a distinct subset of patients with diverse survival situation compared to those single BC or OC. Nonetheless, comprehensive research on their clinical characteristics prognosis is lacking. This study conducted retrospective analysis characteristics, outcomes, prognostic factors dual OC patients. We applied the National Cancer Institute Surveillance, Epidemiology, End Results (SEER) database identify (DPBOC) from 2000 2019, divided...

Acceptor-donor-acceptor (A-D-A)-configured molecules with coplanar dithieno[2,3-d:2',3'-d']thieno[3,2-b:3',2'-b']dipyrrole (DTPT) as the core are promising organic semiconductor materials utilized in photovoltaic devices owing to their efficient charge transportation capabilities. In addition optoelectronic applications, they potential photothermal and photodynamic applications due light-absorption properties. This study evaluates utilization of DTPT-based fused-ring-conjugated small strong...

Abstract Diffuse optical tomography (DOT) is a variant of functional near infrared spectroscopy and has the capability mapping or reconstructing three dimensional (3D) hemodynamic changes due to brain activity. Common methods used in DOT image analysis define activation have limitations because selection period relatively subjective. General linear model (GLM)‐based can overcome this limitation. In study, we combine atlas‐guided 3D reconstruction with GLM‐based (i.e., voxel‐wise GLM...

MicroRNA-206 (miR-206) and connexin 43 (Cx43) are related with the distant metastasis of breast cancer. It remains unclear whether regulatory effect miR-206 on Cx43 is involved in cancer.Using quantitative real-time polymerase chain reaction Western blot, expressions were determined cancer tissues, hepatic pulmonary (PM), cell lines (MCF-10A, MCF-7, MDA-MB-231). MCF-7/MDA-M-231 cells transfected lentivirus-shRNA vectors to enhance/inhibit miR-206, then expression was observed. Cell counting...

Synthesis and downsizing of interpenetrated metal–organic frameworks (MOFs) for biomedical applications. Soft X-ray tomography was applied to characterize the cellular uptake a MOF by cells.

Central lymph node metastasis (CLNM) is regarded as a predictor for local recurrence in patients with papillary thyroid carcinoma (PTC) but the role of prophylactic central dissection (CLND) controversial. Our study aims to identify clinical factors associated CLNM and develop nomogram making individualized decisions.The perioperative data 1,054 consecutive between Jan 2019 April 2021, our center, were reviewed analyzed. A total 747 histopathologically confirmed classical PTC included...

Abstract Communications between tumor cells and surrounding immune help shape the immunity continuum. Recent breakthroughs in high-throughput technologies as well computational algorithms had reported many important tumor-immune cell (TIC) communications, which were scattered thousands of published studies impeded systematical characterization TIC communications across cancer. Here, a comprehensive database, TICCom, was developed to model containing 739 experimentally-validated or...

In this paper, a novel reconstruction method is presented for Near Infrared (NIR) 2-D imaging to recover optical absorption coefficients from laboratory phantom data. The main body of work validates new generation highly efficient algorithms called “Globally Convergent Method” (GCM) based upon actual measurements taken brain-shape phantoms. It has been demonstrated in earlier studies using computer-simulated data that type reconstructions stable complex distributions absorption. results...

Background. Estrogen and progesterone receptor status can predict breast cancer patient prognosis treatment sensitivity, but research on low ER PR levels expression balance remains limited. Methods. From January 2010 to October 2016, 283 ER+/PR+/HER2-breast patients who met the inclusion criteria were enrolled divided into H group (ER > 10%, N = 261) L (1% ≤ 22). Groups further HH 10%/PR 20%, 201), HL 10%/ER 1% 20% PR, 60), LH 5), LL 10%/1% 17). The was excluded due its small size,...

Chronic oxidative stress resulting from hyperlipidemia is thought to be a key pathogenic driver of pancreatic β-cell dysfunction in leading the onset type 2 diabetes mellitus (T2DM). Long non-coding RNAs (lncRNAs) have been increasingly recognized regulate within β-cells context T2DM. In present study, we sought comprehensively analyze roles lncRNAs dysfunctional and mouse islets. Analyses INS-1E cells were performed by RNA-seq qRT-PCR after treating with or without 0.5 mM palmitate for 4...

Background Cholangiocarcinoma is an aggressive carcinoma with increasing incidence and poor outcomes worldwide. Genomic instability alternative splicing (AS) events are hallmarks of development progression. The relationship between genomic instability, AS events, tumor immune microenvironment remain unclear. Methods profiles patients cholangiocarcinoma were obtained from Cancer Genome Atlas (TCGA) spliceSeq database. transcriptomics, simple nucleotide variation (SNP) clinical data TCGA...

Osteoarthritis (OA) is the most common form of arthritis and leading cause old age disability, affecting an estimated 302 million people worldwide. OA seriously overlooked in world. The awareness popularization standardized diagnosis treatment are all lacking. Knees, hips, hands commonly affected joints OA. Based on experience treatment, consensus guidelines, we formulated this standard order to standardize We hope that our can reduce misdiagnosis mistreatment improve prognosis...

Background Our study aimed to evaluate the safety and immunogenicity of third (booster) dose COVID-19 vaccine for patients with endocrine-related cancers. Methods This observational involved 94 breast cancer patients, 92 thyroid 123 healthy individuals who had received vaccine. Data on adverse effects, serum anti-receptor binding domain (RBD)-immunoglobulin (Ig) G, neutralizing antibodies (NAbs) were collected prospectively. Results The anti-RBD-IgG NAb titers significantly lower...

Connexin 43 (Cx43) has been closely linked to the occurrence and progression of breast cancer. Distant metastasis cancer is aided by epithelial-mesenchymal transition circulating tumor cells (CTCs). However, impact Cx43 expression on CTCs extent its role in disease remain unclear.We determined 156 patients, who had with a course two or more years. We also measured CTCs. The were detected blood 139 these patients. These patients divided into groups: group non-Cx43 based their...

Laparoscopic radical prostatectomy (LRP) has revolutionized the surgical treatment of prostate cancer. This procedure permits complete removal and seminal vesicles while minimizing pain recovery time. However, laparoscopic approach greatly limits surgeon's tactile sensation during procedure. is particularly true with robot-assisted LRP where no feedback available forcing surgeon to rely solely on visual cues. The pathologist perform intraoperative frozen section pathologic analysis a few...