A5017949240- Chemical Synthesis and Analysis
- Click Chemistry and Applications
- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Immune Response and Inflammation
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Peptides and Activities
- Crystallography and molecular interactions
- Microbial Metabolic Engineering and Bioproduction
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- Synthetic Organic Chemistry Methods
- Carbohydrate Chemistry and Synthesis
- Lysosomal Storage Disorders Research
- Biochemical and Molecular Research
- Biochemical and biochemical processes
- Catalysis for Biomass Conversion
- Influenza Virus Research Studies
- Chemical synthesis and alkaloids
- Antimicrobial Resistance in Staphylococcus
- Veterinary medicine and infectious diseases
- Viral Infections and Immunology Research
- Multicomponent Synthesis of Heterocycles
- Alkaloids: synthesis and pharmacology
- RNA regulation and disease
Centre National de la Recherche Scientifique
1989-2024
Université de Montpellier
2016-2024
Inserm
2017-2022
Institut de Génomique Fonctionnelle
2017-2022
University of Leeds
2017-2019
Institute of Structural and Molecular Biology
2017-2019
Université Paris-Sud
2014-2017
Université Paris-Saclay
2015-2017
Institut de Chimie Moléculaire et des Matériaux d'Orsay
2013-2017
Laboratoire de Synthèse Organique
2014-2016
A versatile, rapid and reversible approach to constrain peptides in a bioactive helical conformation bearing functional handle for inhibition of protein–protein interactions is described.
Abstract A major current challenge in bioorganic chemistry is the identification of effective mimics protein secondary structures that act as inhibitors protein–protein interactions (PPIs). In this work, trans ‐2‐aminocyclobutanecarboxylic acid ( t ACBC) was used key β‐amino component design α/β/γ ‐ peptides to structurally mimic a native α‐helix. Suitably functionalized α/β/γ‐peptides assume an α‐helix‐mimicking 12,13‐helix conformation solution, exhibit enhanced proteolytic stability...
Abstract Alkaline ceramidases (ACERs) are a class of poorly understood transmembrane enzymes controlling the homeostasis ceramides. They implicated in human pathophysiology, including progressive leukodystrophy, colon cancer as well acute myeloid leukemia. We report here crystal structure ACER type 3 (ACER3). Together with computational studies, reveals that ACER3 is an intramembrane enzyme seven domain architecture and catalytic Zn 2+ binding site its core, similar to adiponectin receptors....
Psychotria douarrei and Geissois pruinosa are known as a hypernickelophore plants. The study of their chemical characteristics was revisited to demonstrate novel potential this natural resource for Green Chemistry. P. showed unique composition, which led concept plant-based catalytic chemistry. supported Biginelli reaction illustrated an interest green organic synthesis.
A Gram-stain-negative, aerobic, rod-shaped, non-spore-forming bacterium (ChimEc512(T)) was isolated from 56 host seedlings of the hyperaccumulating Anthyllis vulneraria legume, which on an old zinc mining site at Les Avinières, Saint-Laurent-Le-Minier, Gard, South France. On basis 16S rRNA gene sequence similarities, strain ChimEc512(T) shown to belong genus Rhizobium and be most closely related endophyticum CCGE 2052(T) (98.4%), tibeticum CCBAU 85039(T) (98.1%), grahamii 502(T) (98.0%)...
A bottom-up design rationale was adopted to devise β/γ-peptide foldamer manifolds which would adopt preferred 13-helix conformations, relying on minimal steric imposition brought by the constituent amino acid residues. In this way, a well-defined conformer revealed for short oligomers of trans-2-aminocyclobutanecarboxylic and γ(4)-amino acids in alternation, gave good topological superposition upon an α-helix motif.
These oligomers adopt a regular zig-zag strand-like secondary structure which does not rely on intra-residue 6-ring hydrogen bonds for stability.
We report the elaboration of novel bio-sourced ecocatalysts for Cu catalyzed azide-alkyne cycloaddition reaction (CuAAC). Ecocatalysis is based on recycling metals issued from rhizofiltration metal rich effluents, and an innovative chemical valorization subsequent biomass in field catalysis. Taking advantage remarkable ability Eichhornia crassipes to accumulate Cu(II) into its roots, these can be directly used preparation copper containing ecocatalysts, called EcoCu, which appear as highly...
Novel band selective decoupled pure shift refocusing experiments allowed simplification of the measurement all δ<sup>1</sup>H, and <italic>J</italic><sub>HH</sub> couplings with an ultrahigh spectral resolution in peptides.
The phosphoprotein (P) is the main and essential cofactor of RNA polymerase (L) non-segmented, negative-strand viruses. P positions viral onto its nucleoprotein-RNA template acts as a chaperone nucleoprotein (N), thereby preventing nonspecific encapsidation cellular RNAs. human metapneumovirus (HMPV) forms homotetramers composed stable oligomerization domain (Pcore) flanked by large intrinsically disordered regions (IDRs). Here we combined x-ray crystallography Pcore with small angle...
Significance Bicomponent leukotoxins represent a promiscuous group among the virulence factors secreted by Staphylococcus aureus that help pathogen to develop and evade host immune system. These toxins specifically recognize G protein–coupled receptors (GPCRs) in an unknown molecular mechanism functional effects on receptor’s biology. Here, using HlgA HlgB, we identify atypical chemokine receptor 1 (ACKR1) as for HlgB demonstrate compete with ACKR1 natural ligand. Furthermore, find binding...
A series of three short oligomers (di-, tri-, and tetramers) cis-2-(aminomethyl)cyclobutane carboxylic acid, a γ-amino acid featuring cyclobutane ring constraint, were prepared, their conformational behavior was examined spectroscopically by molecular modeling. In dilute solutions, these peptides showed number low-energy conformers, including ribbonlike structures pleated around rarely observed intramolecular seven-membered hydrogen bonds. more concentrated interactions defer to an organized...
Foldamers are abiotic molecules that mimic the ability of bio-macromolecules to adopt well-defined and organised secondary, tertiary or quaternary structure. Such templates have enabled generation defined architectures which present structurally surfaces can achieve molecular recognition diverse complex targets. Far less explored is whether this mimicry nature extend more advanced functions biological macromolecules such as activation catalytic function. In work, we a novel replacement...
The design of a β/γ-peptide reveals an unprecedented 9/8-ribbon whose curvature depends on the β-residue configuration and γ-residue conformation.
Hydrogen-bonding interactions are used to bias the conformation of an aromatic oligoamide foldamer in favour α-helix mimicking <italic>syn</italic> conformer.
Abstract A major current challenge in bioorganic chemistry is the identification of effective mimics protein secondary structures that act as inhibitors protein–protein interactions (PPIs). In this work, trans ‐2‐aminocyclobutanecarboxylic acid ( t ACBC) was used key β‐amino component design α/β/γ ‐ peptides to structurally mimic a native α‐helix. Suitably functionalized α/β/γ‐peptides assume an α‐helix‐mimicking 12,13‐helix conformation solution, exhibit enhanced proteolytic stability...
Staphylococcus aureus (SA) leukocidin ED (LukED) belongs to a family of bicomponent pore forming toxins that play important roles in SA immune evasion and nutrient acquisition. LukED targets specific G protein-coupled chemokine receptors lyse human erythrocytes (red blood cells) leukocytes (white cells). The first recognition step is critical for cell targeting lysis. structural molecular bases this mechanism are not well understood but could constitute essential information guide antibiotic...