A5033170867- Research on Leishmaniasis Studies
- Trypanosoma species research and implications
- Parasites and Host Interactions
- Insect Pest Control Strategies
- Fossil Insects in Amber
- Burkholderia infections and melioidosis
- Orthoptera Research and Taxonomy
- Vector-borne infectious diseases
- Insect Resistance and Genetics
- Plant Toxicity and Pharmacological Properties
- Bird parasitology and diseases
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
Institut Pasteur de Tunis
2005-2022
Tunis El Manar University
2014-2022
Tunis University
2014-2018
Institut Pasteur
2001-2006
Instituut voor Tropische Geneeskunde
2001-2002
Eight patients with cutaneous ulcers were referred to the Institute of Endemic Diseases, Khartoum, Sudan, from June 2000 March 2002 for diagnosis suspected leishmaniasis (CL). Diagnosis was confirmed parasitologically by both positive Giemsa-stained smears and successful culture Leishmania promastigotes in NNN medium. The eight parasite isolates shown belong donovani complex kDNA PCR. Isoenzyme typing three revealed that they identical L. MON-82 reference strain, gp63 PCR-RFLP profile showed...
The gp63 encoding genes were characterized by PCR–RFLP in 35 isolates representative of the Leishmania donovani complex ( L. infantum, donovani, archibaldi and chagasi ), with special attention to Mediterranean infantum from different geographical origins, separate groups Old World major, tropica aethiopica ). aim was evaluate how possible selective pressure host on these important surface proteins would influence structuring our sample. Comparison carried out structure obtained (i) reported...
Journal Article Occurrence of Leishmania infantum cutaneous leishmaniasis in central Tunisia Get access Moncef BenSaid, BenSaid aLaboratoire de Parasitologie, Faculté Médecine, Sousse, Search for other works by this author on: Oxford Academic PubMed Google Scholar Souheila Guerbouj, Guerbouj bLaboratoire d'Epidémiologie et d'Ecologie Parasitaire, Institut Pasteur Tunis, BP 74, 1002 Tunis Belvédère, Fatma Saghrouni, Saghrouni Akila Fathallah-Mili, Fathallah-Mili Ikram Guizani ⁎ ⁎Corresponding...
Phlebotomine sand flies were captured from an active transmission focus of sporadic cutaneous leishmaniasis, caused by Leishmania infantum, in El Kef region, northern Tunisia. Both Phlebotomus perniciosus and P. langeroni found. females showed a statistically significant intradomiciliary dominance (P<0.01 for the 2003 2004 seasons) when compared to animal shelters. During season, dissection collected female specimens presence flagellates within digestive tracts two among 1086 observed, but...
Small wild mammals are an important element in the emergence and transmission of vector-borne pathogens (VBPs). Among these species, hedgehogs have been found to be a reservoir VBPs host arthropod vectors. Surveillance wildlife their arthropods crucial one health context. We conducted exploratory study screen Atelerix algirus infesting ticks fleas for using high throughput microfluidic real-time PCR system. Tested biopsies from were naturally infected by Theileria youngi, Hepatozoon sp.,...
Abstract Background Leishmaniasis is endemic in Tunisia and presents with different clinical forms, caused by the species Leishmania infantum , major tropica . The life cycle of complex involves several phlebotomine sand fly vectors mammalian reservoir hosts. aim this work development evaluation a high-resolution melting PCR (PCR-HRM) tool to detect identify parasites wild domestic hosts, constituting confirmed (dogs Meriones rodents) or potential (hedgehogs) reservoirs Tunisia. Methods...
Cutaneous leishmaniasis in Sudan is caused by Leishmania major zymodeme LON1. Self-healing usually occurs within 1 year but occasionally its duration prolonged and treatment required. The clinical forms are ulcers, nodules noduloulcerative lesions. Here we describe seven patients with uncommon lesions that were difficult to recognize as infections. These included mycetoma-like lesions, resembled L. tropica infection others. One HIV/AIDS patient had Kaposi's sarcoma parasites the Kaposi Most...
A gp63PCR method was evaluated for the detection and characterization of Leishmania (Leishmania) (L.) parasites in canine lymph node aspirates. This tool tested compared to other PCRs based on amplification 18S ribosomal genes, a L. infantum specific repetitive sequence kinetoplastic DNA minicircles, classical parasitological (smear examination and/or culture) or serological (IFAT) techniques sample 40 dogs, originating from different endemic regions Tunisia. Sensitivity specificity all PCR...
Visceral leishmaniasis (VL) is mainly due to the Leishmania donovani complex. VL endemic in many countries worldwide including East Africa and Mediterranean region where epidemiology Taxonomy of these pathogens under controversy but there a correlation between their genetic diversity geographical origin. With steady increase genome knowledge, RAPD still useful approach identify characterize novel DNA markers. Our aim was polymorphic markers parasites diverse geographic regions using order...
In Algeria, visceral leishmaniasis (VL) is due to Leishmania (L.) infantum, while three cutaneous forms (CL) are caused by major, tropica and infantum. this study, the use of Giemsa-stained slides was evaluated with two PCR techniques, in Eastern Algeria.A total 136 samples corresponding 100 CL smears (skin scrapings) 36 VL (bone marrow aspirates) collected from 2008 2014 were tested. Upon DNA extraction, PCRs used amplify ribosomal Internal Transcribed Spacer 1 (ITS1) mini-exon genes....
In this study, a negative peanut agglutinin (PNA) selection was used as marker for promastigote differentiation to compare the in vitro growth and kinetics of two visceral cutaneous Leishmania (Leishmania) infantum parasites. All parasites had different kinetics. Cultures initiated with PNA(+) purified during early stationary phase (Day 4), when PNA(-) (non-agglutinating) peaked, yielded high percent. Further morphological analysis at time point showed that 60-86% forms were procyclics,...
Leishmania stocks isolated from cutaneous lesions in Lebanon were characterized by PCR methods. The typed as putative L. (L.) archibaldi (gp63 PCR-RFLP), belonging to 2 different genotypes (PCR-based schizodeme analysis). This constitutes the first report on presence of Middle East.
La femelle de l’espèce Phlebotomus (Larroussius) chadlii Rioux, Juminer & Gibily, 1966, inconnue jusqu’à ce jour, est décrite à partir d’un exemplaire capturé au nord-ouest la Tunisie, dans région du Kef. Elle a pu être distinguée P. ariasi par plusieurs caractères spermathèque : 1) chez chadlii, partie évasée conduit apparaît plus lisse et large que ; 2) cette représente les trois-quart alors elle n’en forme moitié 3) le col court celui ariasi. base compatible avec grande taille valve...
In Tunisia, 17 phlebotomine sand fly species are reported, belonging to genera Phlebotomus and Sergentomyia. However, subsequent faunal studies outbreaks of leishmaniasis in different regions the country, unrecognized could exist. Indeed, we report this study presence Sergentomyia (Parrotomyia) lewisiParrot 1948. A brief collection flies was undertaken August 2016 Khbina locality, Sidi Bouzid governorate situated Central which constitutes an old focus Zoonotic Cutaneous Leishmaniasis. Sand...