A5028642144- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- Inflammasome and immune disorders
- Hippo pathway signaling and YAP/TAZ
- Reproductive System and Pregnancy
- Renal and related cancers
- Prenatal Screening and Diagnostics
- Mesenchymal stem cell research
- Angiogenesis and VEGF in Cancer
- 14-3-3 protein interactions
- Gestational Diabetes Research and Management
- Kruppel-like factors research
- Cell death mechanisms and regulation
- Hearing, Cochlea, Tinnitus, Genetics
- Neonatal Respiratory Health Research
- Interdisciplinary Research and Collaboration
- Congenital Diaphragmatic Hernia Studies
- Wnt/β-catenin signaling in development and cancer
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Cardiovascular Issues in Pregnancy
- Connexins and lens biology
- Pluripotent Stem Cells Research
- Developmental Biology and Gene Regulation
- Interprofessional Education and Collaboration
Women and Children’s Health Research Institute
2013-2024
University of Alberta
2009-2024
Max Planck Institute for Heart and Lung Research
2018-2020
Abstract Immunomodulatory Siglecs are controlled by their glycoprotein and glycolipid ligands. Siglec-glycolipid interactions often studied outside the context of a lipid bilayer, missing complex behaviors glycolipids in membrane. Through optimizing liposomal formulation to dissect Siglec–glycolipid interactions, it is shown that Siglec-6 can recognize independent its canonical binding pocket, suggesting possesses secondary pocket tailored for recognizing bilayer. A panel synthetic...
Enlarged vestibular aqueduct (EVA) is one of the most commonly identified inner ear malformations in hearing loss patients including Pendred syndrome. While biallelic mutations SLC26A4 gene, encoding pendrin, causes non-syndromic with EVA or syndrome, a considerable number appear to carry mono-allelic mutation. This suggests faulty pendrin regulatory machinery results loss. Here we identify EPHA2 as another causative gene syndrome SLC26A4. EphA2 forms protein complex controlling...
Strict regulation of proliferation is vital for development, whereas unregulated cell a fundamental characteristic cancer. The polarity protein atypical kinase C lambda/iota (aPKCλ) associated with through unknown mechanisms. In endothelial cells, suppression aPKCλ impairs despite hyperactivated mitogenic signaling. Here we show that phosphorylates the DNA binding domain forkhead box O1 (FoxO1) transcription factor, gatekeeper growth. Although signaling excludes FoxO1 from nucleus,...
Forskolin is an extract of the Coleus forskholii plant that widely used in cell physiology to raise intracellular cAMP levels. In field trophoblast biology, forskolin one primary treatments induce trophoblastic cellular fusion. The syncytiotrophoblast (ST) a continuous multinucleated human placenta separates maternal from fetal circulations and can only expand by fusion with its stem cell, cytotrophoblast (CT). Functional investigation any aspect ST requires vitro differentiation CT de novo...
The expression of immune checkpoint proteins such as programmed cell death protein 1 ( PD ‐1) and its ligand ‐L1) has been shown to correlate with patient prognosis in many malignant cancers. ‐L1 is controlled by c‐Myc; however, further upstream regulation largely unknown. We have previously that atypical kinase C lambda/iota aPKC λ) phosphorylates the Forkhead box O1 (FoxO1) transcription factor at Ser218 suppress DNA ‐binding ability, thereby regulating c‐Myc controlling physiologic...
Atypical protein kinase-c (aPKC) isoforms are important regulators of polarity and stem cell differentiation. There three aPKC: aPKC-ι, aPKC-ζ, PKM-ζ. Recently, aPKC-ι was shown to regulate human trophoblast (TSC) Compensation by remaining when a single aPKC isoform is lost can occur, but the expression pattern aPKC-ζ in placenta unknown. Here we show that new isoform, III, expressed trophoblasts. Therefore, studies examining role control for potential compensation between necessary...
The syncytiotrophoblast is a human epithelial cell that bathed in maternal blood on the maternal-facing surface of placenta. It therefore acts as barrier and exchange interface between mother fetus. Syncytiotrophoblast dysfunction feature pregnancy pathologies, like preeclampsia. Dysfunctional syncytiotrophoblasts display loss microvilli, which marker aberrant apical–basal polarization, but little data exist about regulation polarity. Atypical PKC isoforms are conserved polarity regulators....
Intrauterine growth restriction (IUGR) is a common complication of pregnancy whereby the fetus fails to achieve its genetic potential. Malformation placental vasculature observed in IUGR and may be due development placenta chronically hypoxic environment. Recently, we identified that predominant stromal cells angiogenic zones are fibrocyte-like cells. The conditioned medium from (FcCM) has been shown stimulate angiogenesis vitro. Thus, hypothesized FcCM would have reduced ability chronic...
Abstract The placenta is an essential organ of pregnancy required for maternal-fetal transport and communication. surface the facing maternal blood formed by a single giant multinucleate cell: syncytiotrophoblast. syncytiotrophoblast maintained via fusion progenitor cytotrophoblasts. Cell-cell tightly regulated process, in non-trophoblastic cells accompanied stereotypical alterations cell shape that have attained fusion-competence. most prominent feature formation actin-based membrane...
In this Perspective, Journal of Cell Science invited researchers working on cell and tissue polarity to share their thoughts unique, emerging or open questions relating field. The goal article is feature 'voices' from scientists around the world at various career stages, bring attention innovative thought-provoking topics interest biology community. These voices discuss intriguing that consider across scales, evolution, development disease. What can yeast protists tell us about evolution in...
Strategic prioritization of research agendas to address health problems with a large social and economic burden has increased the demand for interdisciplinary research. Universities have addressed need in their strategic documents. However, training equip graduates careers teams not kept pace. We offer recommendations graduate students, universities, services organizations, funders designed increase capacity team training, provide an example existing program.
Abstract The syncytiotrophoblast is the placental epithelial cell that forms maternal surface of human placenta, acting as a barrier and facilitating exchange between mother fetus. Syncytiotrophoblast dysfunction feature pregnancy pathologies, like preeclampsia. Dysfunctional display loss microvilli, marker aberrant apical-basal polarization, but little data exists about regulation polarity. Atypical protein kinase-c (aPKC) isoforms are conserved polarity regulators. Thus, we hypothesized...