A5064482913- Pregnancy and preeclampsia studies
- Birth, Development, and Health
- Nitric Oxide and Endothelin Effects
- Gestational Diabetes Research and Management
- Cardiovascular Issues in Pregnancy
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Atherosclerosis and Cardiovascular Diseases
- Estrogen and related hormone effects
- Menopause: Health Impacts and Treatments
- Protein Hydrolysis and Bioactive Peptides
- Adipose Tissue and Metabolism
- Reproductive System and Pregnancy
- Eicosanoids and Hypertension Pharmacology
- Protease and Inhibitor Mechanisms
- Hormonal Regulation and Hypertension
- Cardiovascular Health and Disease Prevention
- Neonatal Respiratory Health Research
- Blood Coagulation and Thrombosis Mechanisms
- Renin-Angiotensin System Studies
- Adipokines, Inflammation, and Metabolic Diseases
- Cardiovascular Disease and Adiposity
- Inflammatory mediators and NSAID effects
- Biochemical effects in animals
- Neonatal Health and Biochemistry
- Heme Oxygenase-1 and Carbon Monoxide
University of Alberta
2016-2025
Women and Children’s Health Research Institute
2016-2025
Spinal Cord Injury Alberta
2022
Kingston General Hospital
2019
University of the West Indies
2019
Centro Rosarino de Estudios Perinatales
2019
Mount Sinai Hospital
2019
Université de Sherbrooke
2019
Ottawa Hospital
2019
Monash University
2019
Abstract —Matrix metalloproteinase-2 (MMP-2, gelatinase A) and its tissue inhibitor (TIMP-2) are mainly known for their roles in the (patho)physiological remodeling of vasculature, angiogenesis, repair, tumor invasion, inflammation, atherosclerotic plaque rupture. A mechanism action MMP-2 is proteolytic breakdown specific extracellular matrix proteins. The amino acid sequences interstitial collagen (Gly-Leu/Ile) laminin-5 (Ala-Leu) that cleaved by homologous to a region (Gly 32 -Leu 33 )...
Abstract The endothelium serves many functional roles, including the modulation of vascular smooth muscle tone through release vasoactive agents such as nitric oxide (NO) and eicosanoids. We proposed that NO produced by endothelial cells would increase production eicosanoids enhanced expression and/or activation prostaglandin H synthase. eicosanoid synthesis were stimulated in a bovine coronary microvessel cell line with calcium ionophore A23187 (1 μmol/L). Our data demonstrated following:...
Abstract —Preeclampsia is a multisystemic disorder of pregnancy in which the normal vascular adaptations to are compromised. Oxidative stress as well endothelial cell dysfunction have been implicated pathophysiological features preeclampsia. Endothelial cells produce vasorelaxant nitric oxide (NO). However, NO also known react with superoxide anions (produced under conditions oxidative stress), yielding peroxynitrite that may impair function. Our objective was use immunohistochemical...
Summary We have recently found matrix metalloproteinase-2 (MMP-2) in human platelets and reported that the release of this enzyme during platelet activation stimulates aggregation. now identified metalloproteinase-9 (MMP-9) resistance-sized (~200 μm) arteries. Resting released small quantities pro-MMP-9. Maximal MMP-9 was detected partial (appr. 30% maximum) aggregation with thrombin. However, maximal MMP-2 associated antibodies induced resting potentiated by thrombin collagen. Moreover,...
Intrauterine growth restriction (IUGR) increases the risk of developing adult-onset cardiovascular disease. We hypothesized that IUGR resulting from maternal hypoxia or nutrient during late gestation will produce cardiac remodeling and impair recovery after ischemia/reperfusion (I/R) in adult male offspring aged 4 7 mo. Sprague-Dawley rats were randomized on day 15 pregnancy to (IUGR-H, 12% oxygen), (IUGR-NR, 40% control diet) (room air) groups. In 4-mo IUGR-H offspring, left ventricular...
It is now recognized that the quality of fetal environment during early development important in programming cardiovascular health and disease later life. Fetal hypoxia one most common consequences complicated pregnancies worldwide. However, contrast to extensive research effort on pregnancy affected by maternal nutrition or stress, contribution chronic developmental only recently becoming delineated established. This review discusses increasing body evidence supporting cardiac...
Intrauterine growth restriction (IUGR), a condition affecting 7-15% of all pregnancies, is associated with an increased mortality rate during adulthood. Several animal models have been developed to study the effects IUGR However, in vivo characteristics these are still unknown. The main aim this work was evaluate, vivo, on cardiopulmonary structure and function adulthood.Pregnant Sprague Dawley rats were exposed hypoxic (12% O2) or normoxic (21% environments between day 15 21 pregnancy....
Egg protein ovotransferrin derived peptides (IRW and IQW) can attenuate tumor necrosis factor (TNF) induced inflammatory responses oxidative stress in endothelial cells. The present study investigates the structural requirements molecular mechanisms underlying these events. Whereas IRW significantly inhibited TNF-induced up-regulation of intercellular cell adhesion molecule-I (ICAM-1) vascular (VCAM-1), IQW could inhibit only ICAM-1. anti-inflammatory effects appeared to be mediated by...
Carbon monoxide, a classical respiratory inhibitor, also exerts vasodilatory, anti-inflammatory, and antiapoptotic effects. CO-releasing molecules have therapeutic value, increasing phagocytosis reducing sepsis-induced lethality. Here we identify for the first time bacterial targets of Ru(CO)(3)Cl(glycinate) (CORM-3), ruthenium-based carbonyl that liberates CO rapidly under physiological conditions. Contrary to expectation would be preferentially inhibitory at low oxygen tensions or...
The search for new molecules to fight Pseudomonas aeruginosa is of paramount importance. Carbon monoxide (CO) known act as an effective inhibitor the respiratory chain in P. aeruginosa, but practical use this gas antibacterial molecule hampered by its toxicity and difficulty manipulate. Here, we show that a water-soluble CO releaser (CORM-3) possesses bactericidal properties against laboratory antibiotic-resistant aeruginosa. CORM-3 reduced bacterial count 4 logs 180 min after vitro...
Background There is a growing interest in using functional food components as therapy for cardiovascular diseases such hypertension. We have previously characterized tri-peptide IRW (Ile-Arg-Trp) from egg white protein ovotransferrin; this peptide showed anti-inflammatory, anti-oxidant and angiotensin converting enzyme (ACE) inhibitor properties vitro. Given the pathogenic roles played by angiotensin, oxidative stress inflammation spontaneously hypertensive rat (SHR), we tested therapeutic...
OBJECTIVE It is recognized that there a remarkable variability in the systemic response to high-fat (HF) diets cannot be completely explained by genetic factors. In addition, pregnancy complications leading intrauterine growth restriction (IUGR) have been associated with an increased risk of developing metabolic syndrome (MetS) later life. Thus, we hypothesized offspring born IUGR exhibit permanent changes make them more susceptible HF diet–induced MetS. RESEARCH DESIGN AND METHODS SD rats...
Preeclampsia and fetal growth restriction are responsible for the majority of maternal perinatal morbidity mortality associated with complicated pregnancies. Although their etiologies complex multifactorial, both increased uterine artery resistance. Sildenafil citrate is able to rescue dysfunction observed ex vivo in arteries women preeclampsia. The ability sildenafil increase vasodilation, thereby decreasing resistance and, hence, ameliorated preeclampsia restriction, was tested a mouse...
Preeclampsia, fetal growth restriction, and miscarriage remain important causes of maternal perinatal morbidity mortality. These complications are associated with reduced numbers a specialized T lymphocyte subset called regulatory cells (Treg cells) in the circulation, decidua, placenta. Treg suppress inflammation prevent immunity toward fetus, which expresses foreign paternal alloantigens. demonstrated to contribute vascular homeostasis, but whether influence adaptations essential for...
To determine the efficacy of high dose folic acid supplementation for prevention pre-eclampsia in women with at least one risk factor: pre-existing hypertension, prepregnancy diabetes (type 1 or 2), twin pregnancy, a previous body mass index ≥35.Randomised, phase III, double blinded international, multicentre clinical trial.70 obstetrical centres five countries (Argentina, Australia, Canada, Jamaica, and UK).2464 pregnant factor were randomised between 2011 2015 (1144 to group 1157 placebo...