A5105457571- Nitric Oxide and Endothelin Effects
- Platelet Disorders and Treatments
- Cardiac Imaging and Diagnostics
- Graphene and Nanomaterials Applications
- Coronary Interventions and Diagnostics
- Polymer Surface Interaction Studies
- Nanoparticle-Based Drug Delivery
- Cardiac Ischemia and Reperfusion
- Antiplatelet Therapy and Cardiovascular Diseases
- Cardiac Fibrosis and Remodeling
- Neuroscience of respiration and sleep
- Ultrasound in Clinical Applications
- Cerebrovascular and Carotid Artery Diseases
- Cardiovascular Health and Disease Prevention
- Cardiovascular Issues in Pregnancy
- Protease and Inhibitor Mechanisms
- Nanoparticles: synthesis and applications
- Blood Coagulation and Thrombosis Mechanisms
- Orthopaedic implants and arthroplasty
- Mechanical Circulatory Support Devices
- Acute Myocardial Infarction Research
- Electromagnetic Launch and Propulsion Technology
- Advanced Drug Delivery Systems
- Education and Critical Thinking Development
- Blood donation and transfusion practices
University of Saskatchewan
1999-2024
University of Pittsburgh Medical Center
2023
University of Pittsburgh
2020
Warsaw University of Technology
2015
Trinity College Dublin
2009
Nicolaus Copernicus University
2005
Collegium Medicum in Bydgoszcz
2005
Silesian Center for Heart Disease
2005
The University of Texas Health Science Center at Houston
2005
University of Alberta
1997-2004
Ever increasing use of engineered carbon nanoparticles in nanopharmacology for selective imaging, sensor or drug delivery systems has increased the potential blood platelet-nanoparticle interactions. We studied effects and combustion-derived on human platelet aggregation vitro rat vascular thrombosis vivo. Multiplewall (MWNT), singlewall (SWNT) nanotubes, C60 fullerenes (C60CS) mixed (MCN) (0.2-300 microg ml(-1)) were investigated. Nanoparticles compared with standard urban particulate...
Background —Matrix metalloproteinases (MMPs) contribute to collagen degradation and remodeling of the extracellular matrix after myocardial infarction; however, their role in dysfunction immediately ischemia reperfusion is unknown. Methods Results —We measured release MMPs into coronary effluent isolated, perfused rat hearts during aerobic perfusion ischemia. Aerobically control expressed pro-MMP-2 MMP-2, as well an unidentified 75-kDa gelatinase. These enzymes were also detected effluent....
Summary We have recently found matrix metalloproteinase-2 (MMP-2) in human platelets and reported that the release of this enzyme during platelet activation stimulates aggregation. now identified metalloproteinase-9 (MMP-9) resistance-sized (~200 μm) arteries. Resting released small quantities pro-MMP-9. Maximal MMP-9 was detected partial (appr. 30% maximum) aggregation with thrombin. However, maximal MMP-2 associated antibodies induced resting potentiated by thrombin collagen. Moreover,...
The development of novel oral drug delivery systems is an expanding area research and both new approaches for improving their efficacy the investigation potential toxicological effect are crucial should be performed in parallel. Polystyrene nanoparticles (NPs) have been used production diagnostic therapeutic nanosystems, widely food packaging, also served as models investigating NPs interactions with biological systems. mucous gel layer that covers epithelium gastrointestinal system a...
Objective Although bleeding associated with thrombocytopenia often complicates extracorporeal membrane oxygenation (ECMO), the mechanisms of platelet dysfunction during ECMO remain poorly understood. We investigated role matrix metalloproteinase (MMP)-2, which recently has been shown to mediate a novel pathway aggregation, in induced by ECMO. Design Prospective longitudinal case study. Setting Level III neonatal intensive care unit. Patients Ten neonates treated Intervention procedure....
The effects of nitric oxide (NO) and metalloproteinases (MMP‐2 MMP‐9) in the pathogenesis hyperoxia‐induced lung damage newborn rats were examined. Three‐day‐old rat pups subjected to hyperoxia (95% O 2 ) or room air for 7 14 days. Some animals treated with N G ‐ L ‐nitro‐ ‐arginine methyl ester ( ‐NAME, 10 mg kg −1 , s.c., daily). Histology, morphometry, oedema, Ca 2+ ‐dependent ‐independent NO synthase (NOS) activities, expression NOS isoforms activities MMP‐2 MMP‐9 measured lungs...
We studied the effects of silver nanoparticles (AgNPs) on human blood platelet function. hypothesized that AgNPs, a known antimicrobial agent, can be used as blood-compatible, "ideal material'' in medical devices or drug delivery system. Therefore, aim current study was to investigate if functionalized AgNPs affect function and platelets well endothelial cell viability vitro.AgNPs, with reduced glutathione (GSH), polyethylene glycol (PEG) lipoic acid (LA) were synthesized. Quartz crystal...
Pancreatic cancer is characterized by one of the lowest five-year survival rates. In search for new treatments, some studies explored several metal complexes as potential anticancer drugs. Therefore, we investigated three newly synthesized oxidovanadium(IV) with 2-methylnitrilotriacetate (bcma3-), N-(2-carbamoylethyl)iminodiacetate (ceida3-) and N-(phosphonomethyl)-iminodiacetate (pmida4-) ligands compounds using pancreatic cell lines. We measured: Cytotoxicity...
Insulin resistance is associated with an increased risk of cardiovascular disease that probably related to abnormalities vascular wall function. The JCR:LA-cp rat a unique animal model human exhibits profound insulin resistance, vasculopathy, and disease, allows study the relationships between Conductance arteries serve different functions, thus may affect these types artery differently. Concentration-response curves norepinephrine, phenylephrine, acetylcholine were studied in conductance...
Abstract Hypoxic injury of cardiovascular system is one the most frequent complications following ischaemia. Heart arises from increased degradation contractile proteins, such as myosin light chains (MLCs) and troponin I by matrix metalloproteinase 2 (MMP‐2). The aim current research was to study effects 5‐phenyloxyphenyl‐5‐aminoalkyl nitrate barbiturate (MMP‐2‐inhibitor‐NO‐donor hybrid) on hearts subjected ischaemia/reperfusion (I/R) injury. Primary human cardiac myocytes Wistar rat...
Background . High on-aspirin treatment platelets reactivity (HPR) is a significant problem in long-term secondary prevention of cardiovascular events. We hypothesize that imbalance between MMPs/TIMPs results disorders. also explored whether chronically elevated blood glucose affects MMP-2/TIMP-4 release from platelets. Materials and Methods Seventy patients with stable coronary artery disease, supplemented aspirin, participated this pilot study. The presence HPR and/or diabetes mellitus was...
Interactions of graphene oxide (GO) with an
Nanoparticles (NPs) are released from orthopedic and neurosurgical prostheses can interact with a number of blood components once in the bloodstream. Potential toxic effects Co Cr NPs on platelets have not been thoroughly investigated. The aim this study was to analyze effect platelet function vitro. ability tested induce activation aggregation measured using light transmission aggregometry, flow cytometry, quartz crystal balance dissipation (QCM-D). This confirmed by electron microscopy...
Research indicates that appropriately designed educational interventions may impact positively on moral reasoning competencies development (MRCD) as measured by a psychometric measure known the Defining Issues Test (DIT). However, findings include intended to MRCD do not consistently promote measurable pre-post development. This paper reviews theoretical background use of MRCD, and spotlights how underpinning Neo-Kohlbergian theory might inform design an intervention in order optimise...
OPEN ACCESSSeptember 25, 2020Use of Ultrasound in Introducing Anatomical Pathology to Preclinical Medical Students, Correlation with Physical Exam Curricula Lauren Trembley, MD, Marek Radomski, DO MD Clinical Instructor, Department Emergency Medicine, University Pittsburgh E-mail Address: [email protected] Google Scholar More articles by this author , Assistant Professor, https://doi.org/10.15766/mep_2374-8265.10950 SectionsAboutAbstractPDF ToolsDownload Citations ShareFacebookTwitterEmail...
Graphene oxide’s (GO) intravascular applications and biocompatibility are not fully explored yet, although it has been proposed as an anticancer drug transporter, antibacterial factor or component of wearable devices. Bivalent cations the number particles’ atom layers, well their structural oxygen content pH dispersion, all affect GO size, shape, dispersibility biological effects. Bovine serum albumin (BSA), important blood plasma protein, is expected to improve dispersion stability in...