Biomaterials based on polyelectrolyte complexation are an innovative concept of coatings and packaging production to be applied in a wide range food products. The aim this study was obtain characterize sodium alginate⁻chitosan complex material with variable degree polyion interactions by oppositely charged polysaccharides. In order complexes, theromogravimetric analysis (TGA), dynamic mechanical thermal (DMTA), nuclear magnetic resonance (NMR), Fourier transform infrared spectroscopy...

Tissue regeneration using human adipose derived mesenchymal stem cells (hASCs) has significant potential as a novel treatment for many degenerative bone and joint diseases. Previous studies have established that age negatively affects the proliferation status osteogenic chondrogenic differentiation of cells. The aim this study was to assess age‐related maintenance physiological function hASCs in vitro. were isolated from patients four different groups: (1) >20 years ( n = 7), (2) >50...

Human adipose tissue is a great source of autologous mesenchymal stem cells (hASCs), which are recognized for their vast therapeutic applications. Their ability to self-renew and differentiate into several lineages makes them promising tool cell-based therapies in different types degenerative diseases. Thus it crucial evaluate age-related changes hASCs, as the elderly group that will benefit most from considerable potential. In this study we investigated effect donor age on growth kinetics,...

The widespread epidemic of obesity and type 2 diabetes (T2D), suggests that both disorders are closely linked. Several pre-clinical clinical studies have showed adipose-derived mesenchymal stem cells (ASC) transplantation is efficient safe. Moreover, scientists already highlighted the therapeutic capacity their secretomes. In this study, we used quantitative PCR, a flow cytometry-based system, ELISA method, spectrophotometry, confocal scanning electron microscopy, to compare differences in...

Due to its pleiotropic effects, the commonly used drug metformin has gained renewed interest among medical researchers. While is mainly for treatment of diabetes, recent studies suggest that it may have further application in anticancer and antiaging therapies. In this study, we investigated proliferative potential, accumulation oxidative stress factors, osteogenic adipogenic differentiation potential mouse adipose‐derived stem cells (MuASCs) isolated from mice treated with 8 weeks....

Abstract Background The senescence of dermal fibroblasts (DFLs) leads to an imbalance in the synthesis and degradation extracellular matrix (ECM) proteins, presenting so-called senescence-associated secretory phenotype (SASP), which ultimately skin aging. Recently, mesenchymal stem cell (MSC)-derived vesicles (EVs) have been recognized as a promising cell-free therapy for degenerative diseases, opens new avenue aging treatment. Methods In this study, we utilized chitosan (CS) hydrogel...

The aim of this work study was to evaluate the cytophysiological activity equine adipose-derived stem cells (ASCs) cultured under conditions static magnetic field. Investigated were exposed a field (MF) with intensity 0.5 T. In order investigate effects on cell signaling, localization and density content microvesicles (MVs) as well morphology, ultrastructure, proliferation rate ASCs evaluated. Results showed that potential mesenchymal accelerated when applied. Resazurin-based assay indicated...

Currently, equine metabolic syndrome (EMS), an endocrine disease linked to insulin resistance, affects increasing number of horses. However, little is known about the effect EMS on mesenchymal stem cells that reside in adipose tissue (ASC). Thus it crucial evaluate viability and growth kinetics these cells, particularly terms their application regenerative medicine. In this study, we investigated proliferative capacity, morphological features, accumulation oxidative stress factors isolated...

The effects of helium and argon plasma treatments on inactivation both pure bacterial cultures inoculated onto the surface agarized media microbiota meat were investigated. Cold plasmas generated by high voltage discharge at low pressure (20 kPa) for 2, 5, 10 min. number viable microorganisms was determined using a plate count method. Morphological changes observed scanning electron microscopy (SEM). Microbial log reduction depended time exposure type gas used. After 10-min treatment with...

Aging and sedentary lifestyle are common nowadays associated with the increasing number of chronic diseases. Thus, physical activity is recommended as one three healthy behavior factors that play a crucial role in health prophylaxis. In present study, we were interested whether influences potential bone-marrow-derived mesenchymal stem cells BMMSCs. this four-week-old male C57Bl/6 mice trained on treadmill at progressive speeds over 5-week period. Comparisons made between exercised (EX)...

Abstract Human adipose tissue is a great source of adult mesenchymal stem cells ( MSC s) which are recognized from their ability to self‐renew and differentiation into multiple lineages. s have promised vast therapeutic potential in treatment many diseases including injury immune disorders. However, regenerative profoundly depends on patients’ age. Age‐related deterioration associated with cellular senescence mainly caused by increased DNA methylation status, accumulation oxidative stress...

Equine Metabolic Syndrome (EMS) is a steadily growing life-threatening endocrine disorder linked to insulin resistance, oxidative stress, and systemic inflammation. Inflammatory microenvironment of adipose tissue constitutes the direct milieu for various cell populations, including adipose-derived mesenchymal stromal cells (ASCs), widely considered as potential therapeutic source in course treatment metabolic disorders. Moreover, elevated stress induces inflammation intestinal epithelial...

Adipose-derived mesenchymal stem cells (ASC) hold great promise in the treatment of many disorders including musculoskeletal system, cardiovascular and/or endocrine diseases. However, cytophysiological condition cells, used for engraftment seems to be fundamental factor that might determine effectiveness clinical therapy. In this study we investigated growth kinetics, senescence, accumulation oxidative stress factors, mitochondrial biogenesis, autophagy and osteogenic differentiation...

Abstract Obesity and endocrine disorders have become prevalent issues in the field of both human veterinary medicine. Equine metabolic syndrome is a complex disorder involving alternation metabolism chronic systemic inflammation. It has been shown that unfavourable microenvironment inflamed adipose tissue negatively affects stem cell population ( ASC ) residing within, markedly limiting their therapeutic potential. s EMS are characterized by increased senescence apoptosis, excessive...

Metformin, the popular anti-diabetic drug was shown to exert multiple biological effects. The most recent metformin gained attention as an agent that mobilizes endogenous progenitor cells and enhances regenerative potential of organisms, for example by promoting neurogenesis. In present study, we examined role on mouse olfactory ensheathing (mOECs) derived from animals receiving eight weeks at a concentration equal 2.8 mg/day. mOECs expanded ex vivo were characterized in terms their cellular...

A main symptom of equine metabolic syndrome (EMS) in ponies is pathological obesity characterized by abnormal accumulation fat deposits and inflammation.In this study, we analyzed the expression two pro-inflammatory cytokines, interleukin-6 (IL-6) tumor necrosis factor-alpha (TNF-), subcutaneous adipose tissue correlation with serum concentrations peripheral blood Welsh ponies.Based on clinical examination findings, animals were divided into groups: affected EMS (n = 8) obese 8).The was...

Endurance exercise has been reported to increase the number of circulating hematopoietic stem/progenitor cells (HSPCs) in peripheral blood (PB) as well bone marrow (BM). We therefore became interested whether endurance same effect on very small embryonic‐like stem (VSELs), which have described a population developmentally early residing BM. Mice were run daily for 1 hour treadmill periods 5 days or weeks. Human volunteers had trained long‐distance running one year, six times per week....

In the present study, a comprehensive approach to biosorption process was proposed. Biosorption of Cr(III), Mn(II) and Mg(II) ions by freshwater macroalga Cladophora glomerata examined using several advanced techniques including FTIR (Fourier Transform Infrared Spectroscopy), ICP-OES (Inductively Coupled Plasma–Optical Emission Spectrometry) SEM-EDX (Scanning Electron Microscopy with Energy Dispersive X-Ray Spectroscopy). The enriched biomass can become valuable, bioactive feed additive for...

Nowadays, endocrine disorders have become more frequent in both human and veterinary medicine. In horses, reduced physical activity combined with carbohydrate sugar overload may result the development of so-called equine metabolic syndrome (EMS). EMS is characterized by insulin resistance, hyperinsulinemia, elevated blood triglyceride concentrations usually obesity. Although phenotypic features individuals are well known, molecular mechanism underlying disease remains elusive. Therefore,...