Human adipose tissue is a great source of autologous mesenchymal stem cells (hASCs), which are recognized for their vast therapeutic applications. Their ability to self-renew and differentiate into several lineages makes them promising tool cell-based therapies in different types degenerative diseases. Thus it crucial evaluate age-related changes hASCs, as the elderly group that will benefit most from considerable potential. In this study we investigated effect donor age on growth kinetics,...

The aim of this work study was to evaluate the cytophysiological activity equine adipose-derived stem cells (ASCs) cultured under conditions static magnetic field. Investigated were exposed a field (MF) with intensity 0.5 T. In order investigate effects on cell signaling, localization and density content microvesicles (MVs) as well morphology, ultrastructure, proliferation rate ASCs evaluated. Results showed that potential mesenchymal accelerated when applied. Resazurin-based assay indicated...

Aging and sedentary lifestyle are common nowadays associated with the increasing number of chronic diseases. Thus, physical activity is recommended as one three healthy behavior factors that play a crucial role in health prophylaxis. In present study, we were interested whether influences potential bone-marrow-derived mesenchymal stem cells BMMSCs. this four-week-old male C57Bl/6 mice trained on treadmill at progressive speeds over 5-week period. Comparisons made between exercised (EX)...

Equine Metabolic Syndrome (EMS) is a steadily growing life-threatening endocrine disorder linked to insulin resistance, oxidative stress, and systemic inflammation. Inflammatory microenvironment of adipose tissue constitutes the direct milieu for various cell populations, including adipose-derived mesenchymal stromal cells (ASCs), widely considered as potential therapeutic source in course treatment metabolic disorders. Moreover, elevated stress induces inflammation intestinal epithelial...

Abstract Obesity and endocrine disorders have become prevalent issues in the field of both human veterinary medicine. Equine metabolic syndrome is a complex disorder involving alternation metabolism chronic systemic inflammation. It has been shown that unfavourable microenvironment inflamed adipose tissue negatively affects stem cell population ( ASC ) residing within, markedly limiting their therapeutic potential. s EMS are characterized by increased senescence apoptosis, excessive...

Metformin, the popular anti-diabetic drug was shown to exert multiple biological effects. The most recent metformin gained attention as an agent that mobilizes endogenous progenitor cells and enhances regenerative potential of organisms, for example by promoting neurogenesis. In present study, we examined role on mouse olfactory ensheathing (mOECs) derived from animals receiving eight weeks at a concentration equal 2.8 mg/day. mOECs expanded ex vivo were characterized in terms their cellular...

A main symptom of equine metabolic syndrome (EMS) in ponies is pathological obesity characterized by abnormal accumulation fat deposits and inflammation.In this study, we analyzed the expression two pro-inflammatory cytokines, interleukin-6 (IL-6) tumor necrosis factor-alpha (TNF-), subcutaneous adipose tissue correlation with serum concentrations peripheral blood Welsh ponies.Based on clinical examination findings, animals were divided into groups: affected EMS (n = 8) obese 8).The was...

Endurance exercise has been reported to increase the number of circulating hematopoietic stem/progenitor cells (HSPCs) in peripheral blood (PB) as well bone marrow (BM). We therefore became interested whether endurance same effect on very small embryonic‐like stem (VSELs), which have described a population developmentally early residing BM. Mice were run daily for 1 hour treadmill periods 5 days or weeks. Human volunteers had trained long‐distance running one year, six times per week....

MiR-21 is being gradually more and recognized as a molecule regulating bone tissue homeostasis. However, its function not fully understood due to the dual role of miR-21 on bone-forming bone-resorbing cells. In this study, we investigated impact inhibition pre-osteoblastic cells differentiation paracrine signaling towards pre-osteoclasts using indirect co-culture model mouse pre-osteoblast (MC3T3) pre-osteoclast (4B12) cell lines. The in MC3T3 (MC3T3inh21) modulated expression genes encoding...

Abstract Background Progression of senile osteoporosis is associated with deteriorated regenerative potential bone marrow-derived mesenchymal stem/stromal cells (BMSCs). According to the recent results, senescent phenotype osteoporotic strongly correlates impaired regulation mitochondria dynamics. Moreover, due ageing population and growing incidence, more efficient methods concerning BMSCs rejuvenation are intensely investigated. Recently, miR-21-5p was reported play a vital role in...

Equine metabolic syndrome (EMS) is mainly characterized by insulin resistance, obesity, and local or systemic inflammation. That unfriendly environment of adipose tissue has huge impact on stem cells population (ASC) residing within. In the present study, using molecular biology techniques multiple imaging (SEM, FIB‐SEM, confocal microscopy), we evaluated EMS ASC viability chondrogenic differentiation. Moreover, visualized mitochondrial network dynamics in CTRL during control conditions....

The aim of this study was to evaluate the proliferation rate and morphological changes adipose-derived mesenchymal stem cells canine equine origin (Eq- CaAdMSC). Investigated were exposed a static magnetic field (MF) with intensity 0.5 T. Proliferation activity determined Alamar Blue assay. Obtained results, normalized in respect control culture, showed that EqAdMSC MF maintained high status, whereas CaAdMSC cultured presence decreased. Estimations population doubling time (PDT) also...

In this study, we investigated the influence of metformin (MF) on proliferation and viability adipose-derived stromal cells isolated from horses (EqASCs). We determined effect cell metabolism in terms mitochondrial oxidative status. Our purpose was to evaluate derived healthy (EqASCHE) individuals affected by equine metabolic syndrome (EqASCEMS). The were treated with 0.5 μM MF for 72 h. proliferative activity evaluated based measurement BrdU incorporation during DNA synthesis, as well...

Osteoporosis results in a severe decrease the life quality of many people worldwide. The latest data shows that number osteoporotic fractures is becoming an increasing international health service problem. Therefore, new kind controllable treatment methods for extensively desired. For reason, we have manufactured and evaluated nanohydroxyapatite (nHAp)-based composite co-doped with iron oxide (IO) nanoparticles. biomaterial was used as matrix controlled delivery miR-21-5p miR-124-3p, which...

The objective of this study was to determine biocompatibility zirconia-based coatings obtained by the sol-gel method. Two matrices, ZrO2 and SiO2/ZrO2, were created applied on stainless steel type 316L with dip-coating technique. morphology topography biomaterials' surface characterized using energy-dispersive X-ray spectroscopy atomic force microscopy, while chemical composition analyzed Raman spectroscopy. Additionally, wettability free energy characterized. Biocompatibility biomaterials...

Mesenchymal stem cells (MSCs) are frequently used in both human and veterinary medicine because their unique properties, such as modulating the immune response differentiating into multiple lineages, make them a valuable tool cell-based therapies. However, many studies have indicated age-, lifestyle-, disease-related deterioration of MSC regenerative characteristics. it still needs to be elucidated how patient's health status affects effectiveness differentiation. In present study, we...

Metformin, the gold standard in type 2 diabetes treatment, is a drug with multi-faceted effects. Currently, metformin has gained much attention as an agent that may find application regenerative medicine. In this study, we considered its pro-osteogenic function course of vitro osteogenesis multipotent stromal cells derived from rat adipose tissue (rASCs). addition, evaluated effect treatment on bone metabolism model cranial defect nondiabetic rats. study showed introduced to culture medium...

In this study, metal-based biomaterials were functionalized with ascorbic acid (LAA). Two types of substrates used: austenitic steel 316L and titanium Ti6Al4V. Coatings prepared the sol–gel method applied on metal surfaces using dip-coating technique. Ascorbic was delivered SiO2-coating at concentrations 0.1 0.4 M. The morphology coatings determined scanning electron microscope (SEM), whereas their elemental composition by SEM-EDX. Immobilization in confirmed Raman spectroscopy....

Osteosarcoma (OSA) is a naturally occurring, malignant bone tumor in both humans and canines, characterized by aggressive local behavior high propensity for metastasis. Despite advances diagnostic methods therapies, long-term survival rates have remained stagnant, underscoring the great need development of biomarkers serving prognosis diagnosis OSA across species. Biomarkers, molecular indicators disease presence or progression, are pivotal tools oncology, offering potential to determine...