Due to development of nanotechnology and gold nanoparticles (AuNPs) increasing use in different areas medicine, especially oncology, better understanding their potential cytotoxicity is necessary protect patients safety. Shape size AuNPs an important modulator cytotoxicity. Therefore, we investigated the rods (≈39 nm length, 18 width), stars (≈ 215 nm) spheres 6.3 against human fetal osteoblast (hFOB 1.19), osteosarcoma (143B, MG63) pancreatic duct cell (hTERT-HPNE) lines by MTT neutral-red...

There is growing evidence that amorphous silica nanoparticles (SiO₂-NP) can cause an inflammatory response in the lung. We studied vitro effects of exposing human lung submucosal cells to SiO₂-NP various sizes (10, 150, and 500 nm) for 2-24 h. Cell survival, reactive oxygen species (ROS), malondialdehyde (MDA) levels, cytokine production, gene expression, genotoxicity were measured after exposure Calu-3 10SiO₂-NP presence or absence flavanoid fisetin antioxidant enzyme catalase. The resulted...

// Ewelina Zielinska 1 , Agata Zauszkiewicz-Pawlak 2 Michal Wojcik 3 and Iwona Inkielewicz-Stepniak Department of Medical Chemistry, University Gdansk, Debinki 1, 80-211 Poland Histology, Faculty Warsaw, Pasteura 02-093 Correspondence to: Inkielewicz-Stepniak, email: iinkiel@gumed.edu.pl Keywords: pancreas ductal adenocarcinoma cells; silver nanoparticles; autophagy; necroptosis; mitotic catastrophe Received: July 20, 2017 Accepted: October 13, Published: November ABSTRACT Pancreatic...

The development of novel oral drug delivery systems is an expanding area research and both new approaches for improving their efficacy the investigation potential toxicological effect are crucial should be performed in parallel. Polystyrene nanoparticles (NPs) have been used production diagnostic therapeutic nanosystems, widely food packaging, also served as models investigating NPs interactions with biological systems. mucous gel layer that covers epithelium gastrointestinal system a...

Objectives: In dentistry, silver nanoparticles (AgNPs) have drawn particular attention because of their wide antimicrobial activity spectrum.However, controversial information on AgNPs toxicity limited use in oral infections.Therefore, the aim present study was to evaluate antibacterial activities against a panel pathogenic bacteria and bacterial biofilms together with potential cytotoxic effects human gingival fibroblasts 10 nm AgNPs: non-functionalized -uncapped (AgNPs-UC) as well...

Periodontal disease (PD), defined as oral inflammation caused by dental plaque, is an emerging problem. PD may lead to tooth loss, and treatment options are limited. In this study, we designed, synthesized, characterized silver nanoparticles (AgNPs) conjugated with chlorhexidine (AgNPs-CHL) or metronidazole (AgNPs-PEG-MET) determine whether they can be used treat PDs.AgNPs were synthesized transmission electron microscopy, UV-vis spectrometry, thermogravimetric analyses, dynamic light...

Silver nanoparticles (AgNPs) and fluoride (F) are pharmacological agents widely used in oral medicine dental practice due to their anti-microbial/anti-cavity properties. However, risks associated with the co-exposure of local cells tissues these xenobiotics not clear. Therefore, we have evaluated effects AgNPs F on human gingival fibroblast cells.Human (CRL-2014) were exposed and/or at different concentrations for up 24 hours. Cellular uptake was examined by transmission electron microscopy....

Abstract: Titanium dioxide (TiO 2 ) nanoparticles (NPs) are manufactured worldwide for a variety of engineering and bioengineering applications. TiO NPs frequently used as material orthopedic implants. However, to the best our knowledge, biocompatibility their effects on osteoblast cells, which responsible growth remodeling human skeleton, have not been thoroughly investigated. In research reported here, we studied exposing hFOB 1.19 cells (5–15 nm) 24 48 hours. Cell viability, alkaline...

BACKGROUND AND PURPOSE Cancer cells grow without the restraints of feedback control mechanisms, leading to increased cancer cell survival. The treatment is often complicated by lack response chemotherapy chemoresistance and persistent survival tumour cells. In this work we studied role platelets in chemotherapy‐induced death EXPERIMENTAL APPROACH Human adenocarcinoma cells, colonic (Caco‐2) ovarian (59 M) were incubated with 5‐fluorouracil (1–300 µg·mL −1 ) or paclitaxel (1–200 presence...

Pancreatic ductal adenocarcinoma is one of the most aggressive human malignancies, where 5‐year survival rate less than 4% worldwide. Successful treatment pancreatic cancer a challenge for today’s oncology. Several studies showed that increased levels oxidative stress may cause cells damage and death. Therefore, we hypothesized as well nitro‐oxidative mechanisms inducing programmed cell We decided to use silver nanoparticles (AgNPs) (2.6 18 nm) key factor triggering reactive oxygen species...

Abstract: Interactions between blood platelets and nanoparticles have both pharmacological toxicological significance may lead to platelet activation aggregation. Platelet aggregation is usually studied using light aggregometer that neither mimics the conditions found in human microvasculature nor detects microaggregates. A new method for measurement of microaggregation under flow a commercially available quartz crystal microbalance with dissipation (QCM-D) has recently been developed. The...

Background Silver nanoparticles (AgNPs) show strong antibacterial properties, making them excellent candidates to be used in orthopaedic repair and regeneration. However, there are concerns regarding the cytotoxicity of AgNPs molecular mechanisms underlying AgNPs-induced bone cells toxicity have not been elucidated. Therefore, aim our study was explore osteoblast cell death with particular emphasis on role nitric oxide (NO) generated by inducible synthase (iNOS). Methods Result this were...

// Szymon Kowalski 1 , Stanisław Hać 2 Dariusz Wyrzykowski 3 Agata Zauszkiewicz-Pawlak 4 and Iwona Inkielewicz-Stępniak Department of Medical Chemistry, University Gdansk, Poland General, Endocrine Transplantation Surgery, Faculty Histology, Correspondence to: Inkielewicz-Stepniak, email: iinkiel@gumed.edu.pl Keywords: vanadium complexes, pancreatic cancer, necroptosis, mitotic catastrophe, autophagy Received: February 17, 2017 Accepted: May 22, Published: July ABSTRACT The cancer is the...

The crystal structures of three novel oxidovanadium(IV) salts containing the [VO(acac)(oda)]‐ anion (acac = acetylacetonate, oda oxydiacetate) and quinolinium ([QH]+), isoquinolinium ([(isoQ)H+) acridinium ([(acr)H+)] counterions, molecular formulas [QH][VO(acac)(oda)] (1), [(isoQ)H][VO(acac)(oda)](H2O) (2) [(acr)H][VO(acac)(oda)](H2O)2 (3) are reported. Notably, these complexes represent first structurally characterized comprising heteroligand complex anion. A comprehensive physicochemical...

The interconnection between brain function and hyperuricemia remains controversial since the available evidence indicates both potent neuroprotective role of uric its negative cardiovascular metabolic effects, possible prooxidant activity. A mixed (protective risk) effect acid on neurological disorders was assumed. Among neurodegenerative diseases, Alzheimer’s disease most prevalent, causes disability, lacks highly effective treatments. Therefore, this review aims to delineate beneficial...