A5027571153- Multiple Myeloma Research and Treatments
- Microfluidic and Bio-sensing Technologies
- 3D Printing in Biomedical Research
- Protein Degradation and Inhibitors
- Angiogenesis and VEGF in Cancer
- Peptidase Inhibition and Analysis
- Advanced Proteomics Techniques and Applications
- Cancer Cells and Metastasis
- Cellular Mechanics and Interactions
- Ubiquitin and proteasome pathways
- Electrostatics and Colloid Interactions
- Microfluidic and Capillary Electrophoresis Applications
- Metabolomics and Mass Spectrometry Studies
- Protease and Inhibitor Mechanisms
- Platelet Disorders and Treatments
- Innovative Microfluidic and Catalytic Techniques Innovation
- Ultrasound and Hyperthermia Applications
- Cancer, Hypoxia, and Metabolism
- Tissue Engineering and Regenerative Medicine
- Chemokine receptors and signaling
- Inflammatory Biomarkers in Disease Prognosis
- Antiplatelet Therapy and Cardiovascular Diseases
- Cancer Mechanisms and Therapy
- Endoplasmic Reticulum Stress and Disease
- Inhalation and Respiratory Drug Delivery
Mater Misericordiae University Hospital
2017-2024
University College Dublin
2023-2024
Massachusetts General Hospital
2012-2023
Harvard University
2012-2023
22q11 Ireland
2019
Trinity College Dublin
2009-2012
Cardiff University
2004-2009
Journal Article Anastomosis of endothelial sprouts forms new vessels in a tissue analogue angiogenesis Get access Jonathan W. Song, Song Edwin L. Steele Laboratory for Tumour Biology, Department Radiation Oncology, Massachusetts General Hospital and Harvard Medical School, Charlestown, MA 02129, USA Search other works by this author on: Oxford Academic Google Scholar Despina Bazou, Bazou Lance Munn E-mail: munn@steele.mgh.harvard.edu Integrative Volume 4, Issue 8, August 2012, Pages 857–862,...
BACKGROUND AND PURPOSE Cancer cells grow without the restraints of feedback control mechanisms, leading to increased cancer cell survival. The treatment is often complicated by lack response chemotherapy chemoresistance and persistent survival tumour cells. In this work we studied role platelets in chemotherapy‐induced death EXPERIMENTAL APPROACH Human adenocarcinoma cells, colonic (Caco‐2) ovarian (59 M) were incubated with 5‐fluorouracil (1–300 µg·mL −1 ) or paclitaxel (1–200 presence...
Single-walled carbon nanotubes (SWNTs) are promising candidates for a wide range of biomedical applications due to their fascinating properties. However, safety concerns raised on potential human toxicity and the techniques that need be used assess such toxicity. Here, we integrate first time 3D tissue-mimetic models in cytotoxicity assessment purified (p-) oxidized (o-) SWNTs. An established ultrasound standing wave trap was generate cell aggregates, results were compared with traditional...
Multiple myeloma (MM) is characterized by extensive immunoglobulin production leading to an excessive load on protein homeostasis in tumor cells. Aminopeptidases contribute proteolysis catalyzing the hydrolysis of amino acids from proteins or peptides and function downstream ubiquitin–proteasome pathway. Notably, aminopeptidases can be utilized delivery antibody peptide-conjugated drugs, such as melflufen, currently clinical trials. We analyzed expression 39 aminopeptidase genes MM samples...
Multiple Myeloma (MM) is an incurable haematological malignancy caused by uncontrolled growth of plasma cells. MM pathogenesis attributed to crosstalk between cells and the bone marrow microenvironment, where extracellular vesicles (EVs) play a role. In this study, EVs secreted from panel cell lines were isolated conditioned media ultracentrifugation fluorescently stained co-cultured with THP-1 monocyte three displayed differential yet dose-dependent uptake cells, H929 displaying greatest EV...
Summary Due to its low beam current and charge compensation mechanism He‐Ion scanning microscopy is a very promising tool for imaging biological cells. However, obtain relevant information, the method used sample preparation also critical. In this work, we have Carl Zeiss Orion Plus helium‐ion microscope study effect of gold coating on morphology human colorectal adenocarcinoma Caco2 The fixative glutaraldehyde was selective samples investigated. A comparative with standard electron presented.
In the present paper, gene expression analysis of mouse embryonic stem (ES) cells levitated in a novel ultrasound standing wave trap (USWT) (Bazou et al. 2005a) at variable acoustic pressures (0.08-0.85 MPa) and times (5-60 min) was performed. Our results showed that levitation ES highest employed pressure for 60 min does not modify maintain their pluripotency. Embryoid bodies (EBs) also expressed early late neural differentiation markers, which were unaffected by field. suggest...
Endothelial cells (ECs) integrate signals from the local microenvironment to guide their behaviour. RhoA is involved in vascular endothelial growth factor (VEGF)-driven angiogenesis, but its role mechanotransduction during sprouting has not been established. Using dominant negative cell transfections a microfluidic device that recapitulates angiogenic sprouting, we show respond interstitial flow RhoA-dependent manner while invading 3-D extracellular matrix. Furthermore, regulates...
Evidence supports the advantages of inhalation over other drug-administration routes in treatment lung diseases, including cancer. Although data obtained from animal models and conventional vitro cultures are informative, testing efficacy inhaled chemotherapeutic agents requires human-relevant preclinical tools. Such tools currently unavailable. Here, we developed characterized for against non-small-cell cancer (NSCLC). These recapitulated key elements both epithelium tumour tissue, namely...
Angiogenesis requires the coordinated growth and migration of endothelial cells (ECs), with each EC residing in vessel wall integrating local signals to determine whether remain quiescent or undergo morphogenesis. These include vascular factor (VEGF) flow-induced mechanical stimuli such as interstitial flow, which are both elevated tumor microenvironment. However, it is not clear how VEGF signaling mechanobiological activation due flow cooperate during angiogenesis. Here, we show that...
Radiotherapy (RT) is a standard treatment for patients with advanced prostate cancer (PCa). Previous preclinical studies showed that SDF1α/CXCR4 axis could mediate PCa metastasis (most often to the bones) and resistance RT. We found high levels of expression both SDF1α its receptor CXCR4 in primary metastatic tissue samples. In vitro analyses using cells revealed an important role cell invasion but not radiotolerance. Pharmacologic inhibition AMD3100 no efficacy orthotopic bone models....
A 2-dimensional aggregate of C6 neural cells was formed rapidly (within 30 s) in suspension a recently developed 1.5 MHz ultrasound standing wave trap. typical 1 mm diameter contained about 3,500 cells. Spreading membrane occurred between the aggregated The rate spreading tangentially developing intercellular contact area 0.19 µm/min. form suspended changed from one hexagonal arrangement to cell-monolayer-like continuous sheet mostly quadrilateral and pentagonal as cell monolayer on solid...
Abstract Background Gelatine coating was previously shown to effectively reduce the cytotoxicity of CdTe Quantum Dots (QDs) which a first step towards utilising them for biomedical applications. To be useful they also need target-specific can achieved by conjugating with Folic Acid (FA). Results The modification QDs FA via an original "one-pot" synthetic route proved successful range characterisation techniques including UV-visible absorption spectroscopy, Photoluminescence (PL) emission...
Particles or cells suspended in an appropriately designed ultrasound standing wave field can be aggregated at a node to form single monolayer plane that interrogated microscopically. The approach is applied here investigate the temporal development of F-actin and Cx43 distribution gap junctional intercellular communication 2-D chondrocyte aggregates (monolayers) rapidly synchronously formed held suspension trap. Development cytoskeleton confluent layer 'cuboidal' forming aggregate was...
Tumour cells activate and aggregate platelets [tumour cell-induced platelet aggregation (TCIPA)] this process plays an important role in the successful metastasis of cancer cells. To date, most studies on TCIPA have been conducted under no-flow conditions. In study, we investigated real time flow conditions, using ultrasound standing wave trap that allows formation levitation cell clusters suspension, thus mimicking conditions generated by flowing blood.Using 59M adenocarcinoma HT1080...
Tumour cell-induced platelet aggregation (TCIPA) facilitates cancer cell invasion, angiogenesis and the formation of metastatic foci. TCIPA can be modulated by pharmacological inhibitors MMP-2 ADP; however, COX inhibitor aspirin did not prevent TCIPA. In this study, we have tested effects a new group isosorbide-based prodrugs on TCIPA.TCIPA was induced in human platelets mixing with adenocarcinoma or fibrosarcoma cells under no flow conditions. The release gelatinases P-selectin expression...
Background: Engineered nanoparticles (NPs) can induce platelet activation and aggregation, but the mechanisms underlying these interactions are not well understood. This could be due in part to use of devices that study function under quasi-static conditions with low sensitivity measure microaggregation. Therefore, this we investigated pharmacological pathways regulators NP-induced microaggregation flow at nanoscale using quartz crystal microbalance dissipation (QCM-D) compared data thus...
Innate drug sensitivity in healthy cells aids identification of lineage specific anti-cancer therapies and reveals off-target effects. To characterize the diversity responses major hematopoietic cell types, we simultaneously assessed their to 71 small molecules utilizing a multi-parametric flow cytometry assay mapped proteomic basal signaling profiles. Unsupervised hierarchical clustering identified distinct subsets based on cellular lineage. Compared other CD19+/B CD56+/NK were more...