A5086561075- Monoclonal and Polyclonal Antibodies Research
- Mitochondrial Function and Pathology
- Algal biology and biofuel production
- ATP Synthase and ATPases Research
- Photosynthetic Processes and Mechanisms
- Microbial Fuel Cells and Bioremediation
- Glycosylation and Glycoproteins Research
- Microbial Metabolic Engineering and Bioproduction
- Electrochemical sensors and biosensors
- Immune Cell Function and Interaction
- Biodiesel Production and Applications
- Protein purification and stability
- Metabolism and Genetic Disorders
- Asthma and respiratory diseases
- Analytical Chemistry and Chromatography
- Biofuel production and bioconversion
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Antioxidant Activity and Oxidative Stress
- Toxin Mechanisms and Immunotoxins
- Biochemical Acid Research Studies
- Immunodeficiency and Autoimmune Disorders
- Process Optimization and Integration
- Photoreceptor and optogenetics research
- Advanced Biosensing Techniques and Applications
- Galectins and Cancer Biology
Kurchatov Institute
2015-2024
Physico-Technical Institute
2021
All-Russian Scientific Research Institute of Food Biotechnology
1990-1999
Czech Academy of Sciences, Institute of Biotechnology
1992-1998
V.I.Shumakov Federal Research Center of Transplantology and Artificial Organs
1997
Ministry of Health of the Russian Federation
1994
Institute of Bioorganic Chemistry
1982-1985
University of Cologne
1982-1983
The existence of a complete oxidative phosphorylation system (OXPHOS) supercomplex including both electron transport and ATP synthases has long been assumed based on functional evidence. However, no structural confirmation the docking between synthase proton pumps obtained. In this study, cryo-electron tomography was used to reveal supramolecular architecture rat heart mitochondria cristae during synthesis. Respirasome structure in situ were determined using subtomogram averaging. obtained...
To compare the pharmacodynamics of trovafloxacin and ciprofloxacin, three clinical isolates Staphylococcus aureus with different MICs (0.03, 0.15, 0.6 0.1, 0.25, 1.25 mg/L, respectively) were exposed to decreasing concentrations quinolones according their half-lives 9.25 4 h, respectively. With each organism, single doses twice-daily ciprofloxacin designed provide 8-fold ranges ratio area under concentration-time curve (AUC) MIC, 58-466 116-932 (mg x h/L)/(mg/L), The antimicrobial effect was...
Background The structure and function of bacterial nucleoid are controlled by histone-like proteins HU/IHF family, omnipresent in bacteria also founding archaea some eukaryotes.HU protein binds dsDNA without sequence specificity avidly DNA structures with propensity to be inclined such as forks, three/four-way junctions, nicks, overhangs bulges. Sequence comparison thousands known from diverse phyla reveals relation between sequence, DNA–binding properties other features. Methodology...
// Denis Shepelin 1, 2 , Mikhail Korzinkin 3 Anna Vanyushina 4 Alexander Aliper Nicolas Borisov 3, 5 Raif Vasilov Nikolay Zhukov 6 Dmitry Sokov 7 Vladimir Prassolov 8 Nurshat Gaifullin 9 Alex Zhavoronkov 10 Bhupinder Bhullar 11 Anton Buzdin 4, 1 Pathway Pharmaceuticals, Wan Chai, Hong Kong, Kong SAR Group for Genomic Analysis of Cell Signaling Systems, Shemyakin-Ovchinnikov Institute Bioorganic Chemistry, Moscow, Russia First Oncology Research and Advisory Center, Laboratory Bioinformatics,...
Effective choice of anticancer drugs is important problem modern medicine. We developed a method termed OncoFinder for the analysis new type biomarkers reflecting activation intracellular signaling and metabolic molecular pathways. These may be linked with sensitivity to drugs. In this study, we compared experimental data obtained in our laboratory Genomics Drug Sensitivity Cancer (GDS) project testing response transcriptomes various human cell lines. The microarray-based profiling was...
Acquired resistance to chemotherapy and radiation therapy is one of the major obstacles decreasing efficiency treatment oncologic diseases. In this study, on two cell lines (ovarian carcinoma SKOV-3 neuroblastoma NGP-127), we modeled acquired five target anticancer drugs. The cells were grown gradually increasing concentrations clinically relevant tyrosine kinase inhibitors (TKIs) Sorafenib, Pazopanib Sunitinib, rapalogs Everolimus Temsirolimus, for 20 weeks. After weeks culturing,...
In the present study, cryo‐electron tomography was used to investigate localization of 2‐oxoacid dehydrogenase complexes (OADCs) in cardiac mitochondria and mitochondrial inner membrane samples. Two classes ordered OADC cores with different symmetries were distinguished their quaternary structures modeled. One class corresponds pyruvate other α‐ketoglutarate branched‐chain α‐ketoacids. OADCs shown be localized close proximity membrane‐embedded respirasomes, as observed both densely packed...
Abstract The biosynthesis of IgD was studied with special reference to aspects glycosylation in the B1‐8 δ cell line. This line isolated as a spontaneous class switch variant from an IgM‐producing line, B1‐8.μ. B1‐8.δ produces both membrane and secretory IgD. is unusual that there large degree heterogeneity N‐linked glycosylation, most likely involving number glycan sidechains are attached. Similar findings have recently been documented for human Further modifications oligosaccharide side...
Abstract Thermo- and photoisomerization of astaxanthin was investigated in a model system (solutions methanol chloroform), the dynamics isomers esters content analyzed Haematococcus pluvialis green algal cells exposed to factors inducing accumulation. In both systems, isomerization process seems be defined by a) action light (or heat), b) dielectric constant surrounding medium. Upon heating, accumulation Z -isomers occurred during entire incubation period. For first 5 h illumination,...
The spatial organization and the antigenic structure of bacteriorhodopsin molecule in purple membrane were studied by immunochemical techniques. Five monoclonal antibodies directed against exposed parts protein prepared characterized. Antigenic determinants localized polypeptide chain analysis interaction between fragments. was revealed with (i) isolated fragments further modified sequential Edman degradation (ii) derivatives obtained biosynthetically or selective chemical modification....
The results of many experimental and theoretical works indicate that after transport protons across the mitochondrial inner membrane (MIM) in oxidative phosphorylation (OXPHOS) system, they are retained on membrane–water interface nonequilibrium state with free energy excess due to low proton surface-to-bulk release. This well-established phenomenon suggests trapping ensures vectorial lateral from pumps ATP synthases (proton acceptors). Despite key role bioenergetics, molecular mechanism...
Using the envelope glycoprotein B (gB) crystal structure and digital prediction algorithm, B- T-cell antigenic determinants (epitopes) of human herpesvirus 1 (HHV-1), also known as herpes simplex virus (HSV-1), were generated, method for their production in form recombinant proteins was proposed. First, surface topological domain (ectodomain or spike) gB with mapped epitopes analyzed, most stable immunogenic (due to enrichment B-and epitopes) subdomains selected modeling subunit vaccine...