A5085031118- Oral microbiology and periodontitis research
- Salivary Gland Disorders and Functions
- Dental Health and Care Utilization
- Oral Health Pathology and Treatment
- Gut microbiota and health
- Dental Erosion and Treatment
- Probiotics and Fermented Foods
- Microbial Metabolites in Food Biotechnology
- Exercise and Physiological Responses
- Streptococcal Infections and Treatments
- Endodontics and Root Canal Treatments
- Adipose Tissue and Metabolism
- Infective Endocarditis Diagnosis and Management
- Amino Acid Enzymes and Metabolism
- Bacterial Genetics and Biotechnology
- Escherichia coli research studies
- Sulfur Compounds in Biology
- Nosocomial Infections in ICU
- Salmonella and Campylobacter epidemiology
- S100 Proteins and Annexins
- Hydrogen's biological and therapeutic effects
- Nitric Oxide and Endothelin Effects
- SARS-CoV-2 detection and testing
- SARS-CoV-2 and COVID-19 Research
- Ecology and Conservation Studies
Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana
2016-2025
Genomics (United Kingdom)
2021
Academic Center for Dentistry Amsterdam
2014-2016
University of Amsterdam
2014-2016
Institute for Atomic and Molecular Physics
2016
The reduction of nitrate to nitrite by the oral microbiota has been proposed be important for health and results in nitric oxide formation that can improve cardiometabolic conditions. Studies bacterial composition subgingival plaque suggest nitrate-reducing bacteria are associated with periodontal health, but impact periodontitis on capacity (NRC) and, therefore, availability not evaluated. current study aimed evaluate how affects NRC microbiota. First, 16S rRNA sequencing data from five...
The salivary glands actively concentrate plasma nitrate, leading to high nitrate concentrations (5-8 mM) after a nitrate-rich vegetable meal. Nitrate is an ecological factor that can induce rapid changes in structure and function of polymicrobial communities, but the effects on oral microbiota have not been clarified. To test this, saliva 12 healthy donors was collected grow vitro biofilms with without 6.5 mM nitrate. Samples were taken at 5 h (most reduced) 9 (all biofilm formation for...
Recent evidence indicates that the reduction of salivary nitrate by oral bacteria can contribute to prevent diseases, as well increase systemic nitric oxide levels improve conditions such hypertension and diabetes. The objective current manuscript was isolate nitrate-reducing from cavity healthy donors test their in vitro probiotic potential nitrate-reduction capacity (NRC) communities. Sixty-two isolates were obtained five different which 53 confirmed be nitrate-reducers. Ten selected based...
Abstract A few studies indicate that nitrate can reduce dysbiosis from a periodontitis point of view. However, these experiments were performed on samples healthy individuals, and it is unknown if will be effective in periodontal patients, where the presence nitrate-reducing bacteria clearly reduced. The aim this study was to test effect R. aeria (Ra9) subgingival biofilms patients with periodontitis. For this, plaque incubated 5 mM for 7 h ( n = 20) or 50 12 10), achieving ~50% reduction...
Periodontitis is characterized by subgingival biofilm dysbiosis, inflammation and tissue destruction. Current treatment involves mechanical disruption known as non-surgical periodontal therapy (NSPT). This study sought to characterise the impact of on microbial diversity overall community, parallel host in oral cavity. Fourty-two periodontitis patients were included this study, with clinical parameters, plaque saliva samples collected at baseline 90 days after treatment. Salivary cytokines...
Tooth decay starts with enamel demineralization due to an acidic pH, which arises from sugar fermentation by acidogenic oral bacteria. Previous in vitro work has demonstrated that nitrate limits acidification when incubating complex communities for short periods (e.g., 1-5 h), driven changes the microbiota metabolism and/or composition. To test whether a single dose of can reduce derived vivo , 12 individuals received nitrate-rich beetroot supplement, was compared placebo blinded crossover...
Current periodontal treatment involves instrumentation using hand and/or ultrasonic instruments, which are used either alone or in combination based on patient and clinician preference, with comparable clinical outcomes. This study sought to investigate early later changes the subgingival biofilm following treatment, identify whether these were associated outcomes, responded differently compared instruments.This was a secondary-outcome analysis of randomized-controlled trial. Thirty-eight...
Abstract We have previously demonstrated that subgingival levels of nitrate-reducing bacteria, as well the in vitro salivary nitrate reduction capacity (NRC), were diminished periodontitis patients, increasing after periodontal treatment. However, it remains unclear if an impaired NRC can affect systemic health. To determine this, effect nitrate-rich beetroot juice (BRJ) on blood pressure was determined 15 patients before and 70 days treatment (i.e., professional mechanical plaque removal,...
Background and objectives: We have developed a standardized, easy-to-use in vitro model to study single- multiple-species oral biofilms real time through impedance technology, which elucidates the kinetics of biofilm formation 96-well plates, without requirement for any further manipulation. Design Results: Using this system, Streptococcus mutans appear be sugar-dependent highly resistant amoxicilin, an antibiotic pathogen is sensitive planktonic state. Saliva, tongue dental plaque samples...
Periodontitis (PD) shows an association with rheumatoid arthritis (RA) and systemic inflammation. Periodontal pathogens, namely Porphyromonas gingivalis Aggregatibacter actinomycetemcomitans, are proposed to be capable of inducing citrullination peptides in the gingiva, formation anti-citrullinated protein antibodies (ACPAs) within susceptible hosts. Here, we sought investigate whether periodontal treatment influenced inflammation antibody titres P. gingivalis, A. Prevotella intermedia ACPA...
Abstract Intra-oral halitosis usually results from the production of volatile sulfur compounds, such as methyl mercaptan and hydrogen sulfide, by tongue microbiota. There are currently no reports on microbial gene-expression profiles microbiota in halitosis. In this study, we performed RNAseq coating samples individuals with without The activity Streptococcus (including S. parasanguinis ) , Veillonella V. dispar Rothia R. mucilaginosa was associated halitosis-free while Prevotella P. shahi...
In the past, epidemiological studies focused on cavitated stages of caries. The arrival International Caries Detection and Assessment System (ICDAS) in 2004 allowed for clinical measurements initial enamel However, since introduction, most applying ICDAS still have studied diseased population. objective this cross-sectional observational study was to describe early caries a large healthy young adult population determine relationship with diet oral hygiene measures. consisted 268 participants...
Some species of oral bacteria can reduce dietary nitrate to nitrite, which later be converted nitric oxide in the nitrate—nitrite—nitic pathway. Increasing availability blood pressure (BP) and improve exercise performance. Streptococcus salivarius M18 (Streptococcus M18) is a bacteriocin-producing probiotic that known health by inhibiting pathogenic bacteria. However, it presently unclear whether probiotic-induced alterations microbiome will influence circulating levels metabolites BP....
The chemotaxis system enables motile bacteria to search for an optimum level of environmental factors. Salmonella typhimurium senses the amino acid cysteine as attractant and its oxidized dimeric form, cystine, a repellent. We investigated dose-response dependence changes in chemotactic signaling activity upon exposure cystine S. LT2 using vivo fluorescence resonance energy transfer (FRET) measurements. curve response had sigmoidal shape, typical receptor-ligand interactions. However,...
Abstract Background The oral microbiome‐dependent nitrate (NO 3 − )–nitrite 2 )–nitric oxide (NO) pathway may help regulate blood pressure. NO ‐producing bacteria in subgingival plaque are reduced relative abundance patients with untreated periodontitis compared periodontally healthy patients. In patients, the increase several months after periodontal treatment. early effects of treatment on and –NO remain unknown. aim this study was to determine how affects microbiome salivary levels over...