A5061496670- Cancer Cells and Metastasis
- Fibroblast Growth Factor Research
- PI3K/AKT/mTOR signaling in cancer
- Kruppel-like factors research
- Cell death mechanisms and regulation
- Helicobacter pylori-related gastroenterology studies
- Cancer Genomics and Diagnostics
- Gastrointestinal Tumor Research and Treatment
- Gastric Cancer Management and Outcomes
- Physiological and biochemical adaptations
- Metastasis and carcinoma case studies
- Proteoglycans and glycosaminoglycans research
- Cancer, Hypoxia, and Metabolism
- Epigenetics and DNA Methylation
- Genetics, Aging, and Longevity in Model Organisms
- Cancer Research and Treatments
- 3D Printing in Biomedical Research
- Genetic factors in colorectal cancer
- Pancreatic and Hepatic Oncology Research
- Cancer Immunotherapy and Biomarkers
- Birth, Development, and Health
- Mesenchymal stem cell research
- Adipose Tissue and Metabolism
- Cancer-related gene regulation
- Hematopoietic Stem Cell Transplantation
National University Cancer Institute, Singapore
2012-2022
National University of Singapore
2010-2022
University of Hong Kong
2015-2021
Office of Adolescent Health
2021
Queen's University Belfast
2014
National University Health System
2011
Clinical Nutrition Research Centre
2006-2008
Institute of Molecular and Cell Biology
1997-2006
Institute of Molecular and Cell Biology
2004
University of Toronto
1997-2000
The surface marker CD44 has been identified as one of several markers associated with cancer stem cells (CSC) in solid tumors, but its ubiquitous expression many cell types, including hematopoietic cells, hindered use targeting CSCs. In this study, 28 paired primary tumor and adjacent nontumor gastric tissue samples were analyzed for protein expression. Cells that expressed pan-CD44 found to occur at significantly higher frequency tissues. We CD44v8-10 the predominant variant verified role a...
Post-treatment detection of circulating tumour DNA (ctDNA) in early-stage triple-negative breast cancer (TNBC) patients predicts high risk relapse. c-TRAK TN assessed the utility prospective ctDNA surveillance TNBC and activity pembrolizumab with detected [ctDNA positive (ctDNA+)].c-TRAK TN, a multicentre phase II trial, integrated by digital PCR, enrolled residual disease following neoadjuvant chemotherapy, or stage II/III adjuvant chemotherapy. comprised three-monthly blood sampling to 12...
Apoptotic stimuli induce conformational changes in Bax and trigger its translocation from cytosol to mitochondria. Upon assembling into the mitochondrial membrane, initiates a death program through series of events, culminating release apoptogenic factors such as cytochrome c . Although it is known that one key for integrating multiple signals, mechanism by which functions mitochondria remains controversial. We have previously identified modulator apoptosis-1 (MAP-1) Bax-associating protein,...
Gastric carcinogenesis has been well documented in the step-wise histopathological model, known as Correa pathway. Several biomarkers including CD44, Musashi-1 and CD133 have reported putative stem cell (PSC) markers. We investigated expression of PSC markers relation to gastric prognosis chemoresponse. Immunohistochemistry staining was performed cancer (GC) clinical specimens representing different steps samples taken before after neoadjuvant chemotherapy with docetaxel, cisplatin...
The transcription factor RUNX3 functions as a tumor suppressor in the gastrointestinal epithelium, where its loss is an early event carcinogenesis. While acts concurrently mediator of TGF-β signaling and antagonist Wnt, cellular changes that follow their contribution to tumorigenicity are not fully understood. Here, we report Runx3 gastric epithelial cells results spontaneous epithelial-mesenchymal transition (EMT). This produces tumorigenic stem cell-like subpopulation, which remarkably...
ZBTB transcription factors orchestrate gene during tissue development. However, their roles in glioblastoma (GBM) remain unexplored. Here, through a functional screening of genes, we identify that BCL6 is required for GBM cell viability and overexpression associated with worse prognosis. In somatic transgenic mouse model, depletion Bcl6 inhibits the progression KrasG12V-driven high-grade glioma. Transcriptome analysis demonstrates involvement tumor protein p53 (TP53), erythroblastic leukemia...
Hypoxia promotes stem cell maintenance and tumor progression, but it remains unclear how regulates long-term adaptation toward these processes. We reveal a striking downregulation of the hypoxia-inducible histone H3 lysine 9 (H3K9) demethylase JMJD1A as hallmark clinical human germ cell-derived tumors, such seminomas, yolk sac embryonal carcinomas. Jmjd1a was not essential for self-renewal played crucial role suppressor in opposition to hypoxia-regulated oncogenic H3K9 methyltransferase G9a....
Preclinical models of diffuse-type gastric cancer (DGC) that reliably predict clinical activity novel compounds are lacking. To overcome the problem poor tumor cellularity in DGC, we used cells from malignant ascites to establish DGC patient-derived xenograft (PDX) recapitulate primary cancer. Cells PDX model GAGA6 with FGFR2 amplification were sensitive AZD4547, a potent FGFR inhibitor is being clinically evaluated for FGFR-aberrant types. Intermittent vivo treatment tumors AZD4547 gave...
We have studied the effect of 1-(5-isoquinolinylsulfonyl)-2-methylpiperazine (H-7), a protein kinase inhibitor, on regulation apoptosis in human neuroblastoma cell line, SH-SY5Y. H-7 (20-100 μM) induced these cells characterized by DNA fragmentation and chromatin condensation. Immunoblot analyses were performed with specific antibody against BCL-2, BCL-XS/L, BAX, JUNB, c-JUN, ICH-1L, c-FOS, RB, CDK-2, p53. treatment did not significantly alter level proteins exception H-7, but staurosporine,...
In Caenorhabditis elegans,fem-1, fem-2, and fem-3 play pivotal roles in sex determination. Recently, a mammalian homologue of the C. elegans sex-determining protein FEM-1, F1Aα, has been described. Although there is little evidence to link F1Aα determination, FEM-1 both promote apoptosis cells. Here we report identification characterization human FEM-2, hFEM-2. Similar hFEM-2 exhibited PP2C phosphatase activity associated with FEM-3. shows striking similarity (79% amino acid identity) rat...
Evasion of apoptosis plays an important role in colon cancer progression. Following loss the Apc tumor suppressor gene mice, encoding Fem1b is up-regulated early neoplastic intestinal epithelium. a pro-apoptotic protein that interacts with Fas, TNFR1, and Apaf-1, increased expression induces cells. homolog FEM-1, Caenorhabditis elegans negatively regulated by ubiquitination proteasomal degradation. To study regulation progression, we used apoptotis-sensitive SW480 cells, derived from primary...
Abstract Mesenchymal stem cells have been implicated as playing an important role in cell engraftment. Recently, a new pluripotent population of umbilical cord blood (UCB) cells, unrestricted somatic (USSCs), with intrinsic and directable potential to develop into mesodermal, endodermal, ectodermal fates, has identified. In this study, we evaluated the capacity ex vivo expanded USSCs influence homing UCB-derived CD34+ marrow spleen nonobese diabetic/severe combined immunodeficient (NOD/SCID)...
Abstract Valproic acid (VPA) is a histone deacetylase inhibitor previously shown to promote the proliferation and self‐renewal of CD34 + hematopoietic cells. We tested effect VPA in conjunction with selective amplification technology developed by Viacell Inc. Stem cells enriched from frozen cord blood were cultured for 7 d, subjected reselection grown fresh medium further d. Treatment resulted an average two‐fold higher expansion CD45 34 compared control. Furthermore, VPA‐treatment induced...
There is a growing trend for researchers to use in vitro 3D models cancer studies, as they can better recapitulate the complex vivo situation. And fact that progression and development of tumor are closely associated its stromal microenvironment has been increasingly recognized. The establishment such supportive niche vital understanding progress metastasis. mesenchymal origin many cells residing provides rationale include MSCs mimicking neuroblastoma. Here we co-encapsulate co-culture NBCs...
RUNX3 functions as a tumor suppressor in the gastric epithelium, where its inactivation is frequently observed during carcinogenesis. We identified IL23A target gene epithelial cells. This was confirmed series of vitro analyses cell lines. In elucidating underlying regulatory network, we uncovered prominent role for TNF-α/NF-κB pathway activating transcription. Moreover, effect TNF-α markedly augmented by infection Helicobacter pylori, primary cause human gastritis. Of note, H. pylori...
Apoptosis is an evolutionarily conserved process that critical for tissue homeostasis and development including sex determination in essentially all multicellular organisms. Here, we report the cloning of ankyrin repeat-containing protein, termed F1Aα, a yeast two-hybrid screen using cytoplasmic domain Fas (CD95/APO-1) as bait. Amino acid sequence analysis indicates F1Aα has extensive homology to sex-determining protein FEM-1 Caenorhabditis elegans, which required aspects male phenotype....
Sex-specific elimination of cells by apoptosis plays a role in sex determination Caenorhabditis elegans. Recently, mammalian pro-apoptotic protein named F1Aα has been identified. shares extensive homology throughout the entire with C. elegans protein, FEM-1, which is essential for achieving all aspects male phenotype nematode. In this report, FEM-1 was investigated. Overexpression induces caspase-dependent cells. cleaved vitro caspase, CED-3, generating an N-terminal cleavage product that...
The expression of winter flounder liver‐type antifreeze protein (wflAFP) genes is tissue‐specific and under seasonal hormonal regulation. only intron the major wflAFP gene was demonstrated to be a liver‐specific enhancer in both mammalian cell lines hepatocytes. Element B, core sequence, shown interact specifically with liver‐enriched transcription factor, CCAAT/enhancer‐binding α (C/EBPα), as well presumptive enhancer‐binding (AEP). In this study, identity rat AEP ortholog revealed via its...