An optoelectronic microdevice is set up to drive single microparticles and a maximum synchronous velocity (MS-velocity) spectrum method proposed for quantifying the frequency-dependent behaviors of individual neutral from 40 kHz 10 MHz. Dielectrophoretic three types are investigated under optically induced nonuniform electric field. Different MS-velocity spectra different particles experimentally found. Numerical calculations polystyrene performed. The MS-velocities specific particle mainly...

Physical scaffolds are useful for supporting cells to form three-dimensional (3D) tissue. However, it is non-trivial develop a scheme that can robustly guide self-organize into tissue with the desired 3D spatial structures. To achieve this goal, rational regulation of cellular self-organization in extracellular matrix (ECM) such as hydrogel needed. In study, we integrated Turing reaction–diffusion mechanism process and produced multicellular structures configurations manner. By optimizing...

Three-dimensional (3D) in vitro tissue models provide an approach for the systematic, repetitive, and quantitative study of drugs. In this study, we constructed 3D acrylated hyaluronic acid (AHA) hydrogel model encapsulating fibroblasts, performed long-period culture, tested cellular topological changes proliferation variation presence herpes simplex virus-1 (HSV-1) as infecting virus acyclovir (ACV) treatment drug. The AHA hydrogels were formed by using Michael addition chemistry...

The interactions between substrate materials and cells usually play an important role in the hydrogel-based 3D cell cultures. However, hydrogels that are used could not be parametrically regulated, especially for quantitatively regulating spatial distribution of adhesion sites 3D. Here, we employed semisynthetic hydrogel consisting maleimide-dextran, Arg-Gly-Asp (RGD) peptides, degradable crosslinkers to biochemically characterize evolutionary behaviors NIH-3T3 fibroblasts C2C12 Moreover, by...

Abstract Human mesenchymal stem cells (hMSCs) are one of the most promising candidates for cell-based therapeutic products. Nonetheless, their biomechanical phenotype after in vitro expansion is still unsatisfactory, example, restricting efficiency microcirculation delivered hMSCs further cell therapies. Here, we propose a scheme using maleimide-dextran hydrogel with locally varied stiffness microscale to modify properties three-dimensional (3D) niches. We show that spatial micro-variation...

Abstract Stiffness of the extracellular matrix plays an important role in regulating cell migration. In this paper, two types 3D dextran hydrogel, with stiffness distributed homogeneously and heterogeneously subcellular scales, have been designed fabricated to serve as macromolecule scaffolds which C2C12 cells, immortalized mouse myoblast line, can migrate a rationally controllable manner. The experimental results indicate that heterogeneous gels support higher migration velocities,...

Biomaterials can serve as the cornerstone of extracellular matrices (ECMs) in three-dimensional (3D) cell culture. Designing diverse ECM materials with adjustable inner structures exhibits growing superiority inducing varied cellular behaviors, such spreading, proliferation, migration, differentiation, and apoptosis. However, a rational easy-to-implement strategy for regulating behaviors during self-organization 3D hydrogels is still lacking. In this study, we propose that...

Four-dimensional (4D) biofabrication techniques aim to dynamically produce and control three-dimensional (3D) biological structures that would transform their shapes or functionalities with time, when a stimulus is imposed cell post-printing self-assembly occurs. The evolution of 3D branching patterns via cells critical for the 4D artificial organs tissues branched geometry. However, it still unclear how formation these are biologically encoded. Here, we study lung utilizing simulation model...

A method incorporating the optically induced electrorotation (OER) and alternating current electroosmotic (ACEO) effects, for formation motion control of microparticle chains, is numerically experimentally demonstrated. In this method, both rotating electric field ACEO fluid roll are generated around border between light dark area fluidic chamber in an optoelectronic tweezers (OET) device. The experimental results show that particle chains can self-rotate their pitch axes under produced due...

Self-organization process of cells involves complicated orchestration various chemical, physical and mechanical cues, the underlying mechanism is still poorly understood. Here, we computationally study three-dimensional (3D) pattern formation vascular mesenchymal around interfaces between hydrogels with different properties. In this dynamic system, interact Bone Morphogenic Protein-2 (BMP-2) Matrix Gla Protein (MGP), which serve as a pair activator inhibitor. A reaction-diffusion model...

Cellular self-organization in extracellular matrix (ECM) is a complex process highly related to ECM proteins, cytokines, growth factors, cell-cell contacts, and mechanical stimuli, study on the underlying mechanism vital for rationally tuning of cells. Here, we investigate 3D pattern formation vascular mesenchymal cells (VMCs) at interfaces between hydrogels with different components mathematical model. A reaction-diffusion model based Turing's instability derived utilized simulate...

We present a theoretical formula to analyze single-mode fiber coupling efficiency of electromagnetic hermite non-uniformly correlated (EMHNUC) beams propagating through the turbulent atmosphere and investigate effects initial beam parameters, including width, coherence length, order, ratio field amplitude on fiber-coupling efficiency. find that initially decreases, increases sharply maximum value, gradually decreases after long propagation distance. This behavior is attributed co-work...

Abstract For mesenchymal stem cell (MSC) therapy to be effective, the vascular system may used deliver and steer cells target tissue. However, expanded MSCs in petri dishes are usually have a limited deformability commonly excluded by capillary networks when homing downstream organs via microcirculation. Here, we propose utilize specially designed 3D dextran hydrogels tuning microscopic heterogeneity of hydrogel composition make administrated mechanically comply with structure mechanics...

An effective technique for automatic separation of microscopic particles through an optoelectronic micro device is experimentally demonstrated. This realized the scanning light patterns at a certain frequency actuating signal this device. electrodeless can dynamically reconstruct distribution electric field, which overcomes drawback that conventional microfluidic devices need costly and rigid microelectrodes or microchannels. The influences voltage width line on pollen particle velocity are...

The recently emerged four-dimensional (4D) biofabrication technique aims to create dynamic three-dimensional (3D) biological structures that can transform their shapes or functionalities with time when an external stimulus is imposed cell postprinting self-assembly occurs. evolution of 3D pattern branching geometry via cells critical for 4D artificial organs tissues branched geometry. However, it still unclear how the formation and these are biologically encoded. We study fabrication lung...