A5034713347- Microfluidic and Bio-sensing Technologies
- Zebrafish Biomedical Research Applications
- Cellular Mechanics and Interactions
- Reproductive Biology and Fertility
- Industrial Technology and Control Systems
- 3D Printing in Biomedical Research
- Image Processing Techniques and Applications
- Piezoelectric Actuators and Control
- Neuroscience and Neural Engineering
- Advanced MEMS and NEMS Technologies
- Force Microscopy Techniques and Applications
- Manufacturing Process and Optimization
- Advanced Vision and Imaging
- Cell Image Analysis Techniques
- Circadian rhythm and melatonin
- Nanowire Synthesis and Applications
- Pluripotent Stem Cells Research
- Indoor and Outdoor Localization Technologies
- Ideological and Political Education
- Surface Modification and Superhydrophobicity
- Simulation and Modeling Applications
- Physics of Superconductivity and Magnetism
- Tissue Engineering and Regenerative Medicine
- Microfluidic and Capillary Electrophoresis Applications
- Effects and risks of endocrine disrupting chemicals
Nankai University
2016-2025
Jiangsu University
2005-2024
University of Electronic Science and Technology of China
2008-2024
Liaoning University of Technology
2007-2024
Southeast University
2008-2024
Shenzhen University
2022-2024
Institute of Robotics
2021-2024
Institute of Microbiology
2024
Chinese Academy of Sciences
2024
Shanghai Jiao Tong University
2024
Left-right (LR) asymmetry is ubiquitous in animal development. Cytoskeletal chirality was recently reported to specify LR embryogenesis, suggesting that tissue morphogenesis coordinated by single- or multi-cell organizers. Thus, organize at multiscale levels of morphogenesis, cells with must also be present adequate numbers. However, observation rarely cultured cells.
Abstract Endogenous retroviruses (ERVs) contribute to ∼10 percent of the mouse genome. They are often silenced in differentiated somatic cells but differentially expressed at various embryonic developmental stages. A minority stem (ESCs), like 2-cell cleavage embryos, highly express ERV MERVL. However, role ERVs and mechanism their activation these still poorly understood. In this study, we investigated regulation function stage-specific ERVs, with a particular focus on totipotency marker...
Abstract Currently, surfactants are widely distributed in the environment. As organic pollutants, their toxicities have drawn extensive attention. In this study, effects of anionic [sodium dodecyl sulphate (SDS) ], cationic [dodecyl dimethyl benzyl ammonium chloride (1227)] and non-ionic [fatty alcohol polyoxyethylene ether (AEO) ] on zebrafish larval behaviour were evaluated. Five behavioural parameters recorded using a rest/wake assay, including rest total, number bouts, bouts length,...
With advances in the development of various disciplines, there is a need to decipher bio-behavioural mechanisms via interdisciplinary means. Here, we present an study role silica nanoparticles (SiO2-NPs) disturbing neural behaviours zebrafish and possible physiological mechanism for this phenomenon. We used adult as animal model evaluate roles size (15-nm 50-nm) concentration (300 μg/mL 1000 μg/mL) SiO2-NP neurotoxicity behavioural analyses. aid video tracking data mining, detected changes...
In this paper, a robotic cell rotation method based on the minimum force is presented to adjust oocyte orientation in biological applications. method, force, which can control angle (RA) of quantitatively and generate deformations, derived through analysis rotation. To exert oocyte, moving trajectories (MT) injection micropipette (IM), are determined using mechanical properties oocytes. Further, by IM along designed MT, achieved. verify feasibility experiment for batch porcine oocytes...
Piezoelectric actuator (PEA) is an ideal microscale and nanoscale because of its ultra-precision positioning resolution. However, the inherent hysteretic nonlinearity significantly degrades PEA’s accuracy. The measured hysteresis PEA exhibits strong rate-dependence saturation phenomena, increasing difficulty in modeling identification. In this paper, a modified Prandtl-Ishlinskii (PI) model proposed. weights backlash operators are updated according to input rates so as account for property....
With increased social pressure, individuals face a high risk of depression. Subsequently, depression affects cognitive behaviour and negatively impacts daily life. Fortunately, the Traditional Chinese Medicine Jia Wei Xiao Yao (JWXY) capsule is effective in reducing improving behaviour.The constituents JWXY were identified by ultra-performance liquid chromatography quadrupole time-of-flight mass spectrometry analyses. We analysed behaviours depression-like zebrafish novel tank with an...
Environmental endocrine chemicals have various adverse effects on the development of vertebrates. Fluorene-9-bisphenol (BHPF), a substitute bisphenol A (BPA), is widely used in commercial production. The BHPF and behavior are unclear. Melatonin plays protective role under many unfavorable conditions. In this study, we investigated behaviors zebrafish whether melatonin reverses induced by BHPF. Zebrafish embryos were exposed to 0.1, 10, or 1000 nmol/L with without 1 μmol/L from 2 hours...
The patch clamp technique has become the gold standard for neuron electrophysiology research in brain science. Brain slices have been widely utilized as targets of due to their higher optical transparency compared a live and intercellular connectivity comparison cultured single neurons. However, narrow working space, small scope, depth field view make positioning operation's micropipette target time-consuming task reliant on high level experience, significantly slowing down operation slices....
Decabromodiphenyl ethane (DBDPE), a widely used new brominated flame retardant, is added into flammable materials to achieve fire retardation. As it continuously detected in the environment, has become an emerging environmental pollutant. However, effects of DBDPE exposure on oocyte maturation and its underlying mechanisms remain unknown. This study found that inhibited rate germinal vesicle breakdown (GVBD), first polar body extrusion (PBE) fertilization mouse oocytes. After 14 h DBDPE,...
We present an integrated micro- and nanofabrication method to create micro/nano dual-scale silicon structures with a controllable sidewall profile over entire 4 inch wafer. The fabrication is based on improved Bosch deep reactive ion etching (DRIE) process its black effect, in which SF6 C4F8 gases are used as the reactants of passivation, respectively. resultant nanostructures have apexes <50 nm diameter, heights 2–3 µm average pitch (i.e. period) ∼400 nm. By regulating etching/passivation...
This paper presents a novel micropipette aspiration (MA) method based on common pneumatic micro-injection system. is the first to quantify influence of capillary effect pressure using balance model, and in return, uses pressure. Subsequently, seal between cell detected judge exclude ineffective MA attempts. The rationality model validated by designed micropipette-filling experiments. Through applied elasticity-determination cells with different sizes, feasibility versatility this are proved....
Abstract In this study, zebrafish larvae are introduced as an in vivo platform to examine the neurotoxicity and developmental toxicity associated with continuous exposure a concentration gradient of different sizes SiO 2 nanoparticles (15 nm 50 diameter) determine dose effect size nanoparticle (NP)‐induced toxicity. Bovine serum albumin (BSA‐V) is utilized stabilizing agent prevent coagulation nanoparticles. To best our knowledge, study first describe locomotor activity assays linking...