A5009049832- Chromosomal and Genetic Variations
- Heat shock proteins research
- Epigenetics and DNA Methylation
- Telomeres, Telomerase, and Senescence
- CRISPR and Genetic Engineering
- Protein Structure and Dynamics
- Natural product bioactivities and synthesis
- Pluripotent Stem Cells Research
- Genomic variations and chromosomal abnormalities
- Chromatography in Natural Products
- Lung Cancer Treatments and Mutations
- Single-cell and spatial transcriptomics
- Insect-Plant Interactions and Control
- Fungal Infections and Studies
- HER2/EGFR in Cancer Research
- Cancer, Hypoxia, and Metabolism
- Synthesis and biological activity
- Antifungal resistance and susceptibility
- Autophagy in Disease and Therapy
- Insect symbiosis and bacterial influences
- Fungal Biology and Applications
- PI3K/AKT/mTOR signaling in cancer
- T-cell and B-cell Immunology
- Medicinal Plant Pharmacodynamics Research
- Genetics, Aging, and Longevity in Model Organisms
Shenyang Medical College
2025
Central Hospital Affiliated to Shenyang Medical College
2025
Fujian Agriculture and Forestry University
2025
State Key Laboratory of Medicinal Chemical Biology
2012-2024
Nankai University
2014-2024
Sichuan University
2012-2024
Zhejiang University
2007-2024
Suzhou Institute of Nano-tech and Nano-bionics
2019-2023
Chinese Academy of Sciences
2019-2023
Guang’anmen Hospital
2022-2023
Tumor ecosystems are composed of multiple cell types that communicate by ligand-receptor interactions. Targeting interactions (for instance, with immune checkpoint inhibitors) can provide significant benefits for patients. However, our knowledge which occur in a tumor and how these affect outcome is still limited. We present an approach to characterize communication across all microenvironment using single-cell RNA sequencing. apply this identify compare the six syngeneic mouse models. To...
ABSTRACT Bemisia tabaci (Hemiptera: Aleyrodidae) is a species complex containing >28 cryptic species, some of which are important crop pests worldwide. Like many other sap-sucking insects, whiteflies harbor an obligatory symbiont, “ Candidatus Portiera aleyrodidarum,” and number secondary symbionts. So far, six genera symbionts have been identified in B. . In this study, we report describe the finding additional bacterium indigenous China 1 (formerly known as biotype ZHJ3). Phylogenetic...
Abstract Endogenous retroviruses (ERVs) contribute to ∼10 percent of the mouse genome. They are often silenced in differentiated somatic cells but differentially expressed at various embryonic developmental stages. A minority stem (ESCs), like 2-cell cleavage embryos, highly express ERV MERVL. However, role ERVs and mechanism their activation these still poorly understood. In this study, we investigated regulation function stage-specific ERVs, with a particular focus on totipotency marker...
Abnormal metabolism and uncontrolled angiogenesis are two important characteristics of malignant tumors. The occurrence both events involves many key molecular changes including miRNA. However, EBV encoded miRNAs rarely mentioned as capable regulating tumor angiogenesis. Here, we reported that one the by EBV, EBV-miR-Bart1-5P, can significantly promote nasopharyngeal carcinoma (NPC) cell glycolysis induces in vitro vivo. Mechanistically, EBV-miR-Bart1-5P directly targets α1 catalytic subunit...
The Cordycipitaceae family of insecticidal fungi is widely distributed in nature, the most complex order Hypocreales (Ascomycota), with members displaying a diversity morphological characteristics and insect host ranges. Based on Bayesian evolutionary analysis five genomic loci(the small subunit ribosomal RNA (SSU) gene, large (LSU) translation elongation factor 1-α (tef1-α) largest polymerase II (rpb1), second (rpb2), we inferred divergence times for Cordycipitaceae, improving internal...
Highlights•Tet enzymes maintain telomere length•Tet chromosomal stability of ESCs•Dnmt3b and 5hmC are involved in Tet-signaling-mediated maintenance•Excessive Zscan4 Dnmt3b lead to heterogeneous elongation lossSummaryTen-eleven translocation (Tet) family proteins convert 5-methylcytosine 5-hydroxymethylcytosine. We show that mouse embryonic stem cells (ESCs) depleted Tet1 and/or Tet2 by RNAi exhibit short telomeres instability, concomitant with reduced recombination. double-knockout ESCs...
Highly conserved molecular chaperone Hsp70 heat shock proteins play a key role in maintaining protein homeostasis (proteostasis). DnaK, major Escherichia coli, has been widely used as paradigm for studying Hsp70s. In the absence of ATP, purified DnaK forms low-ordered oligomer, whereas ATP binding shifts equilibrium toward monomer. Recently, we solved crystal structure complex with ATP. There are two molecules DnaK-ATP asymmetric unit. Interestingly, interfaces between large good surface...
Cellular protein homeostasis depends on heat shock proteins 70 kDa (Hsp70s), a class of ubiquitous and highly conserved molecular chaperone. Key to the chaperone activity is an ATP-induced allosteric regulation polypeptide substrate binding release. To illuminate mechanism this coupling, here we present novel crystal structure intact human BiP, essential Hsp70 in ER, ATP-bound state. Strikingly, polypeptide-binding pocket completely closed, seemingly excluding any binding. Our FRET,...
Ten-eleven translocation (Tet) enzymes are involved in DNA demethylation, important regulating embryo development, stem cell pluripotency and tumorigenesis. Alterations of methylation with age have been shown various somatic types. We investigated whether Tet1 Tet2 regulate aging. showed that Tet1-deficient mice undergo a progressive reduction spermatogonia cells spermatogenesis thus accelerated infertility age. deficiency decreases 5hmC levels downregulates subset genes for cycle, germ...
Telomeres, at the ends of chromosomes, protect chromosomes from fusion and preserve genomic stability. However, molecular mechanisms underlying telomere attrition-induced genome instability remain to be understood. We systematically analyzed expression retrotransposons performed sequencing different cell tissue types with telomeres varying lengths due telomerase deficiency. found that critically short altered retrotransposon activity promote in mouse embryonic stem cells, as evidenced by...
This paper describe the structural optimization of a hit compound, N2-(4-(4-methylpiperazin-1-yl)phenyl)-N8-phenyl-9H-purine-2,8-diamine (1), which is reversible kinase inhibitor targeting both EGFR-activating and drug-resistance (T790M) mutations but has poor binding affinity. Structure–activity relationship studies led to identification 9-cyclopentyl-N2-(4-(4-methylpiperazin-1-yl)phenyl)-N8-phenyl-9H-purine-2,8-diamine (9e) that exhibits significant in vitro antitumor potency against...
The whiteflies under the name Bemisia tabaci (Gennadius) (Aleyrodidae: Hemiptera) are species complex of at least 31 cryptic some which globally invasive agricultural pests. Previously, mitochondrial genome (mitogenome) indigenous New World B. was sequenced and major differences gene order from postulated whitefly ancestral were found. However, sequence mitogenomes in other unknown. In addition, divergences expression profiles remain completely unexplored. this study, we obtained complete...
Lamin A is an inner nuclear membrane protein that maintains structure integrity, involved in transcription, DNA damage response and genomic stability, also links to cell differentiation, senescence, premature aging associated diseases. Induced pluripotent stem (iPS) cells have been successfully generated from various types of used model human It remains unclear whether levels lamin influence reprogramming somatic states during iPS induction. Consistently, expressed more differentiated than...
Feeder cells like mouse embryonic fibroblasts (MEFs) have been widely applied for culture of pluripotent stem cells, but their roles remain elusive. Noticeably, ESCs cultured on the feeders display transcriptional heterogeneity. We investigated feeder by examining telomere maintenance. Here we show that is longer in mESCs with than without feeders. mESC cultures MEF exhibit loss, chromosomal fusion, and aneuploidy increasing passages. Notably, facilitate heterogeneous transcription 2-cell...
A series of 3-(phenylethynyl)-1H-pyrazolo[3,4-d]pyrimidin-4-amine derivatives were designed and synthesized. Structure-activity relationship (SAR) analysis these compounds led to the discovery compound 1j, which showed highest inhibitory potency against Src kinase most potent antiviability activity typical TNBC cell line MDA-MB-231 among all synthesized compounds. Further inhibition assays that 1j was a multikinase inhibitor potently inhibited (IC50 = 0.0009 μM) MAPK signaling protein...
The study of population genetics among the Bemisia tabaci complex is limited due to lack conserved molecular markers. In this study, 358, 433 and 322 new polynucleotide microsatellites are separately identified from transcriptome sequences three cryptic species B. complex. cross transferability 57 was then experimentally validated. results indicate that these markers have high inter-taxon transferability. Thirteen were employed assess genetic relationships six To our surprise, inferred...
Current single-cell RNA-seq approaches are hindered by preamplification bias, loss of strand origin information, and the inability to observe small-RNA mRNA dual transcriptomes. Here, we introduce a holo-transcriptome sequencing (Holo-Seq) that overcomes all three hurdles. Holo-Seq has same quantitative accuracy uniform coverage with complete information as bulk RNA-seq. Most importantly, can simultaneously small RNAs mRNAs in single cell. Furthermore, acquire RNA transcriptomes 32 human...
We herein report the structural optimization and structure–activity relationship studies of 5-(2,3-dihydro-1H-indol-5-yl)-7H-pyrrolo[2,3-d]pyrimidin-4-amine derivatives as a new class receptor-interacting protein kinase 1 (RIPK1) inhibitors. Among all obtained RIPK1 inhibitors, 1-(5-{4-amino-7-ethyl-7H-pyrrolo[2,3-d]pyrimidin-5-yl}-2,3-dihydro-1H-indol-1-yl)-2-[3-(trifluoromethoxy)phenyl]ethan-1-one (22b) is most active one. This compound potently inhibited with binding affinity (KD) 0.004...
Summary Hydrolyzable tannins (HTs), predominant polyphenols in oaks, are widely used grape wine aging, feed additives, and human healthcare. However, the limited availability of a high‐quality reference genome oaks greatly hampered recognition mechanism HT biosynthesis. Here, genomes three Asian oak species ( Quercus variabilis , aliena dentata ) that have different contents were generated. Multi‐omics studies carried out to identify key genes regulating In vitro enzyme activity assay was...
Introduction Entomopathogenic fungi play a crucial role in the ecological regulation of insect populations and can be exploited as resource for pest control, sustainable agriculture, natural products discovery. These their infected hosts are sometimes highly coveted part traditional medicine practices. Here, we sought to examine biodiversity entomogenous subtropical forests China. Methods Fungal-infected specimens were collected from various sites Fujian Province, China, purified isolates...