A5036722319- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Infant Nutrition and Health
- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- T-cell and B-cell Immunology
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Immune Response and Inflammation
- Gut microbiota and health
- Infant Development and Preterm Care
- HIV Research and Treatment
- Monoclonal and Polyclonal Antibodies Research
- Vascular Malformations Diagnosis and Treatment
- Circular RNAs in diseases
- Pediatric health and respiratory diseases
- Teratomas and Epidermoid Cysts
- Birth, Development, and Health
- Adipokines, Inflammation, and Metabolic Diseases
- Diabetes and associated disorders
- MicroRNA in disease regulation
- Arsenic contamination and mitigation
- Reproductive System and Pregnancy
- Developmental Biology and Gene Regulation
- Antimicrobial Peptides and Activities
Instituto Nacional de Perinatología
2017-2024
University of Massachusetts Amherst
2012-2019
Columbia University Irving Medical Center
2017
Columbia University
2016
Center for Research and Advanced Studies of the National Polytechnic Institute
2011-2013
Institut de Biologie Moléculaire et Cellulaire
2013
Max Planck Institute for Molecular Biomedicine
2010
Complejo Hospitalario de Salamanca
2006
Universidad Michoacana de San Nicolás de Hidalgo
2005
Abstract Dendritic cells (DCs) are professional APCs involved in the initiation of both immunity and immunological tolerance. In autoimmune diseases or graft rejections, most reactive lymphocytes effector/memory cells. It is believed that memory T more resistant to tolerance induction than naive lymphocytes; however, studies on mechanisms for their efficient tolerization still scarce. this study, we generated human monocyte-derived DCs by culture with GM-CSF IL-4 (control DCs), as well...
Abstract Microbial colonization of the infant gastrointestinal tract (GIT) begins at birth, is shaped by maternal microbiota, and profoundly altered antibiotic treatment. Antibiotic treatment mothers during pregnancy influences GIT microbiota their infants. The role in regulating adaptive immune function against systemic viral infections infancy remains undefined. We used a mouse model perinatal exposure to examine effect microbial dysbiosis on CD8+ T cell–mediated antiviral immunity....
Severe aplastic anemia (AA) is a bone marrow (BM) failure (BMF) disease frequently caused by aberrant immune destruction of blood progenitors. Although Th1-mediated pathology well described for AA, molecular mechanisms driving progression remain ill defined. The NOTCH signaling pathway mediates Th1 cell differentiation in the presence polarizing cytokines, an action requiring enzymatic processing receptors γ-secretase. Using mouse model we demonstrate that expression both intracellular...
The immune-mediated tissue destruction of graft-vs-host disease (GvHD) remains a major barrier to greater use hematopoietic stem cell transplantation (HSCT). Mesenchymal cells (MSCs) have intrinsic immunosuppressive qualities and are being actively investigated as therapeutic strategy for treating GvHD. We characterized Cymerus™ MSCs, which derived from adult, induced pluripotent (iPSCs), show they display surface markers tri-lineage differentiation consistent with MSCs isolated bone marrow...
Intestinal perforation (IP) associated with necrotizing enterocolitis (NEC) is one of the leading causes mortality in premature neonates; major nutritional and neurodevelopmental sequelae. Since predicting which neonates will develop still challenging; clinicians might benefit considerably an early diagnosis tool identification critical factors. The aim this study was to forecast IP related NEC investigate predictive quality variables; based on a machine learning-based technique....
ABSTRACT Two major subsets of human Mo are identified based on CD14 and CD16 expression: the classical CD16– minor CD14+CD16+ Mo. In vitro studies suggested distinct function differentiation potential for each cell population. However, in vivo relevance these findings remains unclear. To evaluate development an model, we transferred both subpopulations into peritoneum immunocompromised mice homeostatic or inflammatory conditions. Inflammation was induced with soluble LPS particulate zymosan....
RNA interference technology has recently been highlighted as a powerful research method well potential therapeutic treatment for several diseases. However, the delivery of small interfering (siRNA) into T cell lines and primary blood cells is exceedingly challenging, they are resistant to transfection by conventional reagents. As result, there an unmet need nonviral, efficient, easily prepared carriers siRNA hard-to-transfect types. Here, we report novel system based on protein transduction...
We recently reported that maternal antibiotic treatment of mice (MAT) in the last days pregnancy and during lactation dramatically alters density composition gastrointestinal microbiota their infants. MAT infants also exhibited enhanced susceptibility to a systemic viral infection altered adaptive immune cell activation phenotype function. CD8+ effector T cells from consistently demonstrate an inability sustain interferon gamma production vivo following vaccinia virus vitro upon receptor...
Targeting cellular proteins with antibodies, to better understand signaling pathways in the context of disease modulation, is a fast-growing area investigation. Humanized antibodies are increasingly gaining attention for their therapeutic potential, but collection targets limited those secreted from cells or expressed on cell surface. This approach leaves wealth intracellular unexplored as putative antibody binding. Protein kinase Cθ (PKCθ) essential T activation, proliferation, and...
The transmembrane receptor, Notch1 plays an important role during the differentiation of CD4 T cells into helper (Th) subsets in presence appropriate cytokines, including Th1 cells. MicroRNAs have also been shown to be regulators immune responses, negatively regulating cytokine production by miR-29 family microRNAs can act inhibit tbx21 and ifng transcription, two pro-inflammatory genes that are abundantly expressed Here we show may prime responsive Th1-polarizing cues through its early...
Maternal obesity has been related to adverse neonatal outcomes and fetal programming. Oxidative stress adipokines are potential biomarkers in such pregnancies; thus, the measurement of these molecules considered critical. Therefore, we developed artificial neural network (ANN) models based on maternal weight status clinical data predict reliable blood concentrations at end pregnancy. Adipokines (adiponectin, leptin, resistin), DNA, lipid protein oxidative markers (8-oxo-2'-deoxyguanosine,...
Over the last 20 years, incidence of vertical HIV transmission has decreased from 25%–42% to less than 1%. Although there are no signs infection, health HIV-exposed uninfected (HEU) infants is notoriously affected during first months life, with opportunistic infections being most common disease. Some studies have reported effects on transfer antibodies, but little known about subclass distribution these antibodies. We proposed evaluate total IgG concentration and its subclasses in HIV+...
Abstract Introduction HIV‐exposed uninfected (HEU) newborns suffer from higher risks of opportunistic infections during the first months life compared to HIV‐unexposed (HUU) newborns. Alterations in thymic mass, amounts T helper (Th) cells, T‐cell receptor diversity, and activation markers have been found HEU newborns, suggesting alterations cell ontogeny differentiation. However, little is known about ability these cells produce specialized Th responses CD4 + cells. Method To characterize...
(1) Background: The relationship between enteral nutrition and neonatal necrotizing enterocolitis (NEC) among premature neonates is still unclear. present work was designed to assess the NEC feeding strategies compared control infants. (2) Methods: A retrospective case-control study of infants (<35 weeks’ gestation) with or without that examined practices clinical characteristics at birth 3, 7, 14-day hospitalization, a longitudinal cross-sectional analysis. (3) Results: total 100...
Genetic manipulation has increased our understanding of gene function and led to the discovery new therapeutic targets. Cre/LoxP DNA recombination is widely used for genetic studies in mammalian cells. The direct delivery Cre recombinase fused protein transduction domains (PTDs), such as TAT, been described a valid alternative conditional, site-specific expression transgenic mice. However, efficiently conveying proteins into live cells, especially primary T remains major challenge. In this...
Preterm neonates are at high risk of infectious and inflammatory diseases which require antibiotic treatment. Antibiotics influence neonatal gut microbiome development, intestinal dysbiosis has been associated with delayed gastrointestinal transit. Neonates who take less time to pass meconium have a better tolerance enteral feeding. We analyzed the effect treatment on stool pattern oral in 106 preterm infants < 33 weeks gestational age. were classified 3 groups according (ABT) days: no...