A5087977009- Preterm Birth and Chorioamnionitis
- Reproductive System and Pregnancy
- Pregnancy and preeclampsia studies
- Pregnancy-related medical research
- Gestational Diabetes Research and Management
- Antimicrobial Peptides and Activities
- Pregnancy and Medication Impact
- Neonatal and Maternal Infections
- Birth, Development, and Health
- COVID-19 Impact on Reproduction
- Vitamin D Research Studies
- Immune Response and Inflammation
- Neonatal Respiratory Health Research
- Cardiovascular Issues in Pregnancy
- Immune cells in cancer
- Corneal Surgery and Treatments
- Reproductive tract infections research
- Blood Coagulation and Thrombosis Mechanisms
- Adipokines, Inflammation, and Metabolic Diseases
- Infant Nutrition and Health
- Adipose Tissue and Metabolism
- Estrogen and related hormone effects
- Genomics, phytochemicals, and oxidative stress
- Maternal and Perinatal Health Interventions
- Neuroinflammation and Neurodegeneration Mechanisms
Instituto Nacional de Perinatología
2016-2025
Universidad Nacional Autónoma de México
2006-2015
Neonatal sepsis is a major cause of mortality in preterm infants, with Escherichia coli as one the leading pathogens. Few studies have examined interplay between virulence factors, resistance profiles, phylogroups, and clinical outcomes this population. We analyzed 52 E. strains isolated from 49 neonates diagnosed at tertiary-level hospital Mexico. Strains underwent phylogenetic classification, gene profiling, antimicrobial testing. PFGE was used to assess genetic relatedness outbreak...
Chorioamniotic membranes infection is a pathologic condition in which an abnormal secretion of proinflammatory cytokines halts fetal immune tolerance. The aim the present study was to evaluate functional response human chorioamniotic membranes, as well individual contribution amnion and choriodecidua after stimulation with Escherichia coli, pathogen associated preterm labor. Explants from 10 women (37–40 weeks gestation) were mounted cultured Transwell system, allowed us test compartments...
Prolactin (PRL) plays an important role in trophoblast growth, placental angiogenesis and immunomodulation within the feto-maternal interface, where different cell types secrete PRL express its receptor. During pregnancy, inflammatory signalling is a deleterious event that has been associated with poor fetal outcomes. The placenta highly responsive to stimulus; however, actions of immunity inflammation remain largely unknown. aim this study was evaluate effects on TLR4/NFkB cascade targets...
Neonatal sepsis remains difficult to diagnose due its non-specific signs and symptoms. Traditional scoring systems help discriminate between septic or not patients, but they do consider every single patient particularity. Thus, the purpose of this study was develop an early- late-onset neonatal diagnosis model, based on clinical maternal data from electronic records, at time suspicion. A predictive model obtained by training validating artificial Neural Networks (ANN) algorithm with a...
Increased risk of preterm labor has been linked to cervicovaginal infection with Ureaplasma urealyticum and group B streptococci. Although various experimental models have developed study the role amniochorion in labor, they typically exclude initial interaction between intrauterine leukocytes (recruited from decidual vessels into avascular fetal membranes) infecting bacteria. In this work, we ascertained whether inflammatory molecules secreted by bacterium-activated stimulate production...
Problem Infection of human fetal membranes elicits secretion pro‐inflammatory modulators through its innate immune capacities. We investigated the effect lipopolysacharide ( LPS ) and progesterone P 4) upon expression TLR ‐4/ M y D 88, TNF α, IL ‐6, ‐8, ‐10, HBD 2 on amniotic epithelium. Method study Explants epithelium were pre‐treated with 0.01, 0.1, 1.0 μ m 4; then cotreated 1000 ng/mL . ‐4 was immuno‐detected, concentrations quantified by ELISA Results 4 significantly reduced ‐induced...
Citation Zaga‐Clavellina V, Martha RV‐M, Flores‐Espinosa P. In vitro secretion profile of pro‐inflammatory cytokines IL‐1β, TNF‐α, IL‐6, and human beta‐defensins (HBD)‐1, HBD‐2, HBD‐3 from chorioamniotic membranes after selective stimulation with Gardnerella vaginalis. Am J Reprod Immunol 2012; 67: 34–43 Problem Preterm labor associated infection is a major clinical condition; in this work, we analyze the response stimulated vaginalis . Method study Using two‐compartment experimental model,...
During human pregnancy, infection/inflammation represents an important factor that increases the risk of developing preterm labor. The purpose this study was to determine if pre-treatment with progesterone has immunomodulatory effect on placenta production endotoxin-induced inflammation and degradation extracellular matrix markers. Placentas were obtained under sterile conditions from pregnancies delivered at term before onset labor by cesarean section. Explants central cotyledons 10...
Premature rupture of fetal membranes (PROM) complicated with intrauterine infection has been associated to alterations the extracellular matrix (ECM) homeostasis. The aim this work was evaluate integral/functional response amnion (AMN) and choriodecidua (CHD) synthesis, secretion, activity MMP-2 MMP-9 their inhibitors TIMP-1, -2, -4, after stimulation Escherichia coli. Full-thickness were mounted on a Transwell device, constituting two independent chambers, coli (1×10 (6) CFU/mL) added...
Background. Gestational weight gain (GWG) constitutes an essential aspect of the gestational process. Due to factors such as pregestational body mass index (BMI), nutritional intake, level physical activity, and psychological aspects, recommended GWG may not be achieved, leading adverse neonatal outcomes. Adolescents, due their physiological mental developmental stage, are at a higher risk inappropriate GWG. Our aim is highlight importance in our population determine correlation with...
Amniotic membrane (AM) is the inner layer of placenta, which in contact with fetus; it has been used for transplantation ocular surface diseases. It reported that amniotic promotes epithelialization, inhibits angiogenesis and diminishes inflammation. A persistent epithelial defect delay wound healing caused by infiltrating inflammatory cells into cornea successfully its treatment, however mechanism action inhibiting inflammation not well understood. This study was aimed at determining...
Abstract Background During an ascending infection along the reproductive tract, extra-placental membranes must act as a selective and competent barrier against pathogens. Human beta defensins (HBD)1, HBD2, HBD3 are key elements of innate immunity that secreted to neutralize/control progression infection. Methods Full-thickness were mounted on Transwell device, constituted by two independent chambers, 1 × 10(6) CFU/ml Escherichia coli added either amnion (AMN) or choriodecidual (CHD) face...
Interleukin (IL)-10 is a cytokine with anti-inflammatory properties that plays pivotal roles in immune recognition and maintenance of pregnancy, limiting the harmful effects pro-inflammatory modulators. The aim this work was to characterize contribution amnion choriodecidua regions human fetal membranes production IL-10 after selective stimulation Candida albicans, Gardnerella vaginalis Streptococcus agalactiae.Pre-labor were cultured two-compartment tissue culture system stimulated 1 ×...
Objective To evaluate whether progesterone (P4) is able to modulate the secretion of tumour necrosis factor α ( TNF ‐ ), interleukin‐1 β IL ‐1 ‐6, ‐8, ‐10 and matrix metalloproteinase‐9 MMP ‐9) after choriodecidual stimulation with lipopolysaccharide LPS ). Design Chorioamnionitis‐elicited preterm delivery associated an uncontrolled proinflammatory cytokines that may induce s, which modify fine immunological structural equilibrium at fetal–maternal interface. Setting Instituto Nacional de...
Pregnancy is a unique immunological condition in which an "immune-diplomatic" dialogue between trophoblasts and maternal immune cells established to protect the fetus from rejection, create privileged environment uterus simultaneously be alert any infectious challenge. The maternal-placental-fetal interface (MPFI) performs essential role this defense. In review, we will address MPFI as active immuno-mechanical barrier that protects against viral infections. We describe main infections...
During intrauterine infection, amniochorionic membranes represent a mechanical and immunological barrier against dissemination of infection. Human beta defensins (HBD)-1, HBD-2, HBD-3 are key elements innate immunity that the first line defense different pathogen microorganisms associated with preterm labor. The aim this work was to characterize individual contribution amnion (AMN) choriodecidua (CHD) regions secretion HBD-1, HBD-2 HBD-3, after stimulation Candida albicans. Full-thickness...